Lentinula edodes culture medium and preparation method thereof
A culture medium for mushrooms, the technology of shiitake mushrooms, applied in the direction of microorganism-based methods, biochemical equipment and methods, fungi, etc., can solve the problem of reducing the yield and quality of shiitake mushroom strains, the growth of shiitake mushroom hyphae is not vigorous, and the culture medium is susceptible to Prevents bacteria infection and other problems, to achieve the effect of ensuring healthy growth, not easy to be infected by bacteria, and killing bacteria and viruses
Inactive Publication Date: 2018-01-26
段双燕
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[0002] At present, most of the well-known mushroom culture medium is made of a certain proportion of potatoes, glucose, agar, and water, which is commonly known as PDA medium, but this culture medium is prone to infection by miscellaneous bacteria , easy to produce the death of shiitake mushroom hyphae. In addition, because this medium lacks the lignin needed by shiitake mushroom mycelia, the growth of shiitake mushroom hyphae is not vigorous, which reduces the output and quality of shiitake mushroom strains. In addition, the existing shiitake mushroom strains The culture medium is susceptible to bacterial infection
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[0019] Scrape and wash the potatoes first, cut 20 grams of potatoes into thin slices, add 30 grams of pear sawdust, fresh pear sawdust is better, add 1200 grams of water, boil for 3-5 minutes, cool to 30 degrees Celsius , filter with double-layer gauze to remove slag, and obtain a solution; heat the solution to 80 degrees Celsius, add 20 grams of glucose, 25 grams of agar, 0.5 grams of magnesium sulfate, and 1.5 grams of Ganoderma lucidum powder, stir to dissolve, and then divide the solution into different test tubes The solution in each tube accounts for one-third of the volume of the test tube; the test tube containing the solution is vacuum sterilized at 138 degrees Celsius for half an hour, and cooled naturally to normal temperature to obtain the mushroom culture medium of the present invention. Note that when the test tube is taken out of the sterilizer, the test tube should be placed at an angle to increase the liquid surface area for inoculation.
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The invention relates to a lentinula edodes culture medium. The lentinula edodes culture medium is prepared from components in parts by weight shown as follows: 18-22 parts of potatoes, 28-32 parts ofpear saw powder, 18-22 parts of glucose, 24-26 parts of agar, 1150-1250 parts of water, 0.4-0.6 parts of magnesium sulfate, and 1-2 parts of wall-broken ganoderma lucidum powder. Firstly, the potatoes are peeled and chopped, and then boiled together with the pear saw powder; filtration is performed; after filtration, the filtrate is heated and then mixed with other components; the mixture is putinto a test tube; sterilization is performed. The lentinula edodes culture medium provided by the invention can produce the lentinula edodes which has the advantages of excellent culture purity, denseand exuberant hypha, and strong resistance to disease and infectious microbe; the pollution rate is less than 1%; and nutrients are rich. In addition to nutrients of common lentinus edodes, the lentinus edodes produced by the lentinula edodes culture medium also has trace elements and nutrients in the ganoderma lucidum powder, which improve the properties of cancer prevention and resistance, andrealize the lipid-lowering, hypoglycemic functions.
Description
technical field [0001] The invention relates to edible fungus cultivation techniques, in particular to a culture medium for mushroom strains and a preparation method thereof. Background technique [0002] At present, most of the well-known mushroom culture medium is made of a certain proportion of potatoes, glucose, agar, and water, which is commonly known as PDA medium, but this culture medium is prone to infection by miscellaneous bacteria , easy to produce the death of shiitake mushroom hyphae. In addition, because this medium lacks the lignin needed by shiitake mushroom mycelia, the growth of shiitake mushroom hyphae is not vigorous, which reduces the output and quality of shiitake mushroom strains. In addition, the existing shiitake mushroom strains The medium is susceptible to bacterial infection. Contents of the invention [0003] The invention provides a mushroom culture medium and a preparation method thereof. The culture medium for mushroom strains of the inven...
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IPC IPC(8): C12N1/14C12R1/645
Inventor 段双燕
Owner 段双燕
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