Preparation method of low molecular weight chitosan

A low-molecular-weight, chitosan technology, applied in the field of chitosan degradation, can solve the problems of wide molecular weight distribution of products, large irradiation dose of low-molecular-weight chitosan, etc., which is beneficial to physiological activity, shortens irradiation time, molecular weight Narrow distribution of effects

Active Publication Date: 2018-02-02
广州华大生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] At present, the existing irradiation method to prepare low-molecular-weight chitosan has d

Method used

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  • Preparation method of low molecular weight chitosan
  • Preparation method of low molecular weight chitosan
  • Preparation method of low molecular weight chitosan

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Embodiment 1, a kind of preparation method of low molecular weight chitosan

[0035] The preparation method of described low molecular weight chitosan, comprises the following steps:

[0036] S1 Take a hydrogen peroxide solution with a mass concentration of 5%, add water-soluble humic acid, the quality of the water-soluble humic acid is 0.2% of the above-mentioned hydrogen peroxide solution, and stir evenly to obtain a mixed solution;

[0037] S2 immersing chitosan in the mixed solution obtained in step S1, the mass concentration of chitosan is 25%, soaking for 30min, filtering to obtain filter residue;

[0038]S3 freezes the filter residue obtained in step S2 three times, the first freezing temperature is -4°C, the first freezing time is 2h, the second freezing temperature is -10°C, the second freezing time is 1h, and the third freezing time is -10°C. The freezing temperature is -19°C, and the third freezing time is 40 minutes. After thawing, dry the thawed filter res...

Embodiment 2

[0041] Embodiment 2, a kind of preparation method of low molecular weight chitosan

[0042] The preparation method of described low molecular weight chitosan, comprises the following steps:

[0043] S1 Take a hydrogen peroxide solution with a mass concentration of 12%, add water-soluble humic acid, the quality of the water-soluble humic acid is 0.6% of the above-mentioned hydrogen peroxide solution, and stir evenly to obtain a mixed solution;

[0044] S2 immersing chitosan in the mixed solution obtained in step S1, the mass concentration of chitosan is 35%, soaking for 50min, filtering to obtain filter residue;

[0045] S3 freezes the filter residue obtained in step S2 three times, the first freezing temperature is -4°C, the first freezing time is 4h, the second freezing temperature is -10°C, the second freezing time is 3h, and the third freezing time is -10°C. The freezing temperature is -19°C, and the third freezing time is 60 minutes. After thawing, dry the thawed filter r...

Embodiment 3

[0048] Embodiment 3, a kind of preparation method of low molecular weight chitosan

[0049] The preparation method of described low molecular weight chitosan, comprises the following steps:

[0050] S1 Take a hydrogen peroxide solution with a mass concentration of 10%, add water-soluble humic acid, the quality of the water-soluble humic acid is 0.4% of the above-mentioned hydrogen peroxide solution, and stir evenly to obtain a mixed solution;

[0051] S2 immersing chitosan in the mixed solution obtained in step S1, the mass concentration of chitosan is 30%, soaking for 40min, filtering to obtain filter residue;

[0052] S3 freezes the filter residue obtained in step S2 three times, the first freezing temperature is -4°C, the first freezing time is 3h, the second freezing temperature is -10°C, the second freezing time is 2h, and the third freezing time is -10°C. The freezing temperature is -19°C, and the third freezing time is 50 minutes. After thawing, dry the thawed filter r...

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Abstract

The invention belongs to the technical field of chitosan degradation, and concretely relates to a preparation method of low molecular weight chitosan. The preparation method of the low molecular weight chitosan comprises the following steps: immersing chitosan in a solution containing hydrogen peroxide and water-soluble humic acid for a period of time, performing filtration to obtain filter residues, freezing the filter residues three times, unfreezing the frozen filter residues, drying the unfrozen chitosan in a shady place, grinding the dried chitosan to form fine powder, and irradiating thefine powder at normal temperature under normal pressure. The preparation method of the low molecular weight chitosan can effectively reduce the radiation dosage, and the obtained product has a narrowmolecular weight distribution and a low molecular weight.

Description

technical field [0001] The invention belongs to the technical field of chitosan degradation, and in particular relates to a preparation method of low molecular weight chitosan. Background technique [0002] Chitosan is a natural high molecular polymer, which belongs to aminopolysaccharide, and its scientific name is [(1,4)-2-acetylamino-2-deoxy-β-D-glucose]. Charged alkaline polysaccharides. Chitosan widely exists in lower biological fungi, algal cells, shells of arthropod shrimp, crabs, insects, etc. in nature. Chitosan has good biocompatibility, low toxicity, and biodegradability. Used in food, medicine, health care, bioengineering and other fields. Chitosan has an inhibitory effect on the growth of a variety of bacteria, exhibiting antibiotic-like characteristics. Chitosan can promote skin tissue healing and repair. Chitosan has a hemostatic effect, and chitosan itself has a hemostatic effect. At the same time, chitosan achieves hemostasis through its interaction wit...

Claims

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Application Information

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IPC IPC(8): C08B37/08
CPCC08B37/003
Inventor 邹伟权
Owner 广州华大生物科技有限公司
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