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Fructus schisandrae water-soluble glycoprotein extract as well as preparation method and application thereof

A protein extract, the technology of Schisandra chinensis, applied in the field of water-soluble glycoproteins, can solve the problems that have not yet been reported on the research of Schisandra glycoproteins, and achieve the effect of improving competitiveness and expanding the scope of application

Inactive Publication Date: 2018-02-06
CHANGCHUN UNIV OF CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But so far, there is no research report on Schisandra glycoprotein

Method used

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  • Fructus schisandrae water-soluble glycoprotein extract as well as preparation method and application thereof
  • Fructus schisandrae water-soluble glycoprotein extract as well as preparation method and application thereof
  • Fructus schisandrae water-soluble glycoprotein extract as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] (1) Homogenize the fruit of Schisandra chinensis by adding water according to the material-to-liquid ratio (w / v) of 1:20 to obtain a homogenate;

[0028] (2) Adjust the pH value of the homogenate to 8.0 with 1M NaOH;

[0029] (3) leaching in a water bath at 4°C for 5 hours;

[0030] (4) Centrifuge the extract, collect the supernatant, discard the precipitate, adjust the pH value of the supernatant to 3.2 with 1M Hcl, stir well and let stand for 1 h;

[0031] (5) Centrifuge, discard the supernatant, dissolve the precipitate with a small amount of distilled water to obtain a suspension, adjust the pH value of the suspension to neutral with 1M NaOH, put it into a dialysis bag for dialysis, freeze-dry the inner permeate, and obtain a freeze-dried solution. pink;

[0032] (6) Take a certain amount of freeze-dried powder, dissolve it in distilled water, centrifuge, and collect the supernatant;

[0033] (7) Dissolve the precipitate in distilled water of the same volume, cen...

Embodiment 2

[0036] (1) Homogenize the fruit of Schisandra chinensis by adding water according to the material-to-liquid ratio (w / v) of 1:25 to obtain a homogenate;

[0037] (2) Adjust the pH value of the homogenate to 8.5 with 1M NaOH;

[0038] (3) leaching in a water bath at 30°C for 4 hours;

[0039] (4) Centrifuge the extract, collect the supernatant, discard the precipitate, adjust the pH value of the supernatant to 3.3 with 1M Hcl, stir well and let stand for 1.5h;

[0040] (5) Centrifuge, discard the supernatant, dissolve the precipitate with a small amount of distilled water to obtain a suspension, adjust the pH value of the suspension to neutral with 1M NaOH, put it into a dialysis bag for dialysis, freeze-dry the inner permeate, and obtain a freeze-dried solution. pink;

[0041] (6) Take a certain amount of freeze-dried powder, dissolve it in distilled water, centrifuge, and collect the supernatant;

[0042] (7) Dissolve the precipitate in distilled water of the same volume, c...

Embodiment 3

[0045] (1) Homogenize the fruit of Schisandra chinensis with water according to the material-to-liquid ratio (w / v) of 1:30 to obtain a homogenate;

[0046] (2) Adjust the pH value of the homogenate to 9.0 with 1M NaOH;

[0047] (3) leaching in a water bath at 40°C for 3 hours;

[0048] (4) Centrifuge the extract, collect the supernatant, discard the precipitate, adjust the pH value of the supernatant to 3.4 with 1M Hcl, stir well and let stand for 2 hours;

[0049] (5) Centrifuge, discard the supernatant, dissolve the precipitate with a small amount of distilled water to obtain a suspension, adjust the pH value of the suspension to neutral with 1M NaOH, put it into a dialysis bag for dialysis, freeze-dry the inner permeate, and obtain a freeze-dried solution. pink;

[0050] (6) Take a certain amount of freeze-dried powder, dissolve it in distilled water, centrifuge, and collect the supernatant;

[0051] (7) Dissolve the precipitate in the same volume of distilled water, cen...

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Abstract

The invention relates to a fructus schisandrae water-soluble glycoprotein extract as well as a preparation method and an application thereof and belongs to the medical field. Firstly, active glycoprotein in fructus schisandrae is extracted with an alkali dissolution and acid deposition method, an extracting solution is dialyzed, a dialysis solution is freeze-dried, freeze-dried powder is dissolvedin water, ultrafiltration is performed through an ultrafiltration membrane, a concentrated solution is obtained and finally freeze-dried, and the fructus schisandrae water-soluble glycoprotein is obtained. The glycoprotein has the total sugar content of 67.4% and protein content of 26.3%; the polysaccharide part mainly comprises glucose and also contains mannose, polyuronide, galacturonic acid, rhamnose, galactose, xylose, arabinose and fucose. 16 amino acids are obtained through hydrolysis of the protein part. Pharmacodynamic tests prove that the fructus schisandrae water-soluble glycoprotein has the antioxidant effect, the blank of preparation of fructus schisandrae water-soluble glycoprotein is filled up, the application range of the fructus schisandrae water-soluble glycoprotein in the medical field is extended, and the competitiveness of fructus schisandrae in international markets is improved.

Description

technical field [0001] The invention belongs to the field of traditional Chinese medicines, and in particular relates to a water-soluble glycoprotein extracted from the fruit of Schisandra chinensis. The protein extract has a certain antioxidant effect on human body. Background technique [0002] Schisandra chinensis is the dry and mature fruit of Schisandra chinensis (Turcz.) Baill. and Schisandra sphenanthera Rehd. "Tang Materia Medica" says: "The skin and flesh of the five flavors are sweet and sour, the core is hard, and they all have a salty taste, so the five flavors are also present", hence the name Schisandra. "Compendium of Materia Medica" has a record that "Schisandra is divided into north and south, the color of the south is red, and the color of the north is black, and the north is good when it is used as a medicine". Therefore, the former is called "Northern Schisandra" and the latter is "Southern Schisandra". . Schisandra has the effects of astringing the lun...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/415C07K1/34A23L33/185A23L33/125A61K38/16A61P39/00
CPCC07K14/415A23V2002/00A61K38/00A23V2200/30A23V2250/548
Inventor 严铭铭邵帅李会影万志强赵大庆刘畅田双杨洋
Owner CHANGCHUN UNIV OF CHINESE MEDICINE
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