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Method for culturing canna indica in vitro

A technology of in vitro culture and canna, which is applied in the field of plant cultivation, can solve the problems of susceptibility to infection by various viruses, limited number of seeds, low seed setting rate, etc., and achieve the effect of achieving a large number of multiplication, low cost, and promoting bud growth

Inactive Publication Date: 2018-02-09
广西北流森艺瓷业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, at present, the varieties with high market demand basically have a very low seed setting rate, or even no seed, and the number of seeds is limited. However, the propagation by tuber division is limited by the state of the material itself, the production season and the land. The annual output is far from meeting the market demand.
At the same time, since the long-term propagation material of canna is underground for a long time, it is easy to be infected by various viruses. Since canna mostly adopts asexual reproduction, it is difficult to eradicate it with chemical drugs once it is infected.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Select the rhizomes with buds in the underground of canna as explants, cut the base of the rhizomes with buds into segments with buds, rinse the explants with pure water for 2-3 times, and then wash the explants with a volume concentration of 0.1% zinc blue powder solution Soak in water for 30min, wash with water and wash with 0.15% HgCl 2 Soak the explants in the solution for 15-20 minutes to sterilize the explants, and finally rinse the explants with sterile water, place them on sterile filter paper to absorb the water, and set aside.

[0021] Inoculate the stem section of the treated canna underground rhizome with buds into the induction medium for culture, first culture in the dark for 3-4 days, and then culture in the light, the light time is 12h / d, the light intensity is 500lx, and the culture temperature is 22-25°C , the culture time is 20-22d, and the initial buds of canna are cultivated, and the initial bud induction rate reaches 99%; wherein, the formula of th...

Embodiment 2

[0026] Select the rhizomes with buds in the underground of canna as explants, cut the base of the rhizomes with buds into segments with buds, rinse the explants with pure water for 2-3 times, and then wash the explants with a volume concentration of 0.1% zinc blue powder solution Soak in water for 35min, wash with water and wash with 0.15% HgCl 2 Soak the explants in the solution for 15-20 minutes to sterilize the explants, and finally rinse the explants with sterile water, place them on sterile filter paper to absorb the water, and set aside.

[0027] Inoculate the stem section of the treated canna underground rhizome with buds into the induction medium for culture, first cultivate in the dark for 3-4 days, then cultivate in the light, the light time is 12h / d, the light intensity is 1000lx, and the culture temperature is 25-26°C , the culture time is 20-22 days, and the initial buds of canna are cultivated, and the initial bud induction rate reaches 99%; wherein, the formula ...

Embodiment 3

[0032] Select the rhizomes with buds in the underground of canna as explants, cut the base of the rhizomes with buds into segments with buds, rinse the explants with pure water for 2-3 times, and then wash the explants with a volume concentration of 0.1% zinc blue powder solution Soak in water for 40min, rinse with clean water and use 0.15% HgCl volume concentration 2 Soak the explants in the solution for 15-20 minutes to sterilize the explants, and finally rinse the explants with sterile water, place them on sterile filter paper to absorb the water, and set aside.

[0033] Inoculate the stem section of the treated canna underground rhizome with buds into the induction medium for culture, first culture in the dark for 3-4 days, and then culture in the light, the light time is 12h / d, the light intensity is 1000lx, and the culture temperature is 26-28°C , the culture time is 20-22d, and the initial buds of canna are cultivated, and the initial bud induction rate reaches 99%; whe...

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Abstract

The invention discloses a method for canna in vitro culture, which comprises explant treatment, induction culture, multiplication culture and rooting culture, selects the rhizome with buds underground of canna as the explant, and cuts the base of the rhizome with buds into strips Bud stem section, after the explants are cleaned, sterilized and disinfected, they are inoculated in the induction medium and cultivated to obtain the initial buds of canna, and then the initial buds of canna are transferred to the proliferation medium for proliferation and culture, and finally the differentiated long The clustered shoots of 1-2cm are transferred to the rooting medium and cultivated to obtain rooting canna. The present invention rapidly obtains canna tissue culture seedlings by inducing, proliferating and rooting canna, establishes a good tissue culture rapid propagation system, takes root quickly, shortens the time for canna tissue culture, has low cost, and improves the rooting rate and survival of canna at the same time It has good economic, social and ecological benefits.

Description

technical field [0001] The invention relates to the technical field of plant cultivation, in particular to a method for in vitro cultivation of cannas. Background technique [0002] Canna (Canna indica), also known as Lanjiao and Tanhua, is a plant of the family Cannaceae and the genus Canna. It is a perennial bulbous herb flower native to tropical America and Africa. The plant height of canna can reach 100-180cm. It grows in clusters, with large leaves and rich colors, large flowers, bright colors, various colors, and long flowering period. It is a very good landscaping and landscaping plant. Canna leaves can absorb harmful substances such as sulfur dioxide and hydrogen chloride. Although the leaves are vulnerable, they can regenerate new leaves and quickly resume growth. Therefore, they can be used as indicators for monitoring harmful gas pollution, purifying the air and protecting the environment. In addition, canna can also be used as medicine to treat sores, uterine bl...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/008A01H4/001
Inventor 李海飞
Owner 广西北流森艺瓷业有限公司
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