Quality detecting method for desmodium styracifolium general flavone extract

A quality inspection method, the technology of Desmodium sativa, is applied in the field of quality inspection of the total flavonoids extract of Desmodium sativa, which can solve the problems of large volume, difficult storage, and inconvenient transportation, and achieve high-tech, accurate methods, and high added value. Effect

Inactive Publication Date: 2018-02-09
GUANGXI INST OF CHINESE MEDICINE & PHARMA SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, the medicinal part of Herba sativa is the dry above-ground part, which has the disadvantages of large volume, difficult storage, and inconvenient transportation in application.

Method used

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  • Quality detecting method for desmodium styracifolium general flavone extract
  • Quality detecting method for desmodium styracifolium general flavone extract
  • Quality detecting method for desmodium styracifolium general flavone extract

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Take the dried medicinal material of Desmodium spp. and grind it into coarse powder 10kg, add 80kg80% ethanol for reflux extraction for 2 hours for the first time, add 60kg80% ethanol for 1.5 hours for the second time, filter, combine the filtrate, recover ethanol from the filtrate and concentrate under reduced pressure to be alcohol-free concentrated to a thick paste with a relative density of 1.25 (60~70°C), take the above thick paste (1:4) and put it on the treated macroporous resin (D101) column, first elute with water until it is almost colorless, Then eluted with 10 times the amount of 30% ethanol solution, and finally eluted with 8 times the amount of 50% ethanol solution, collected the ethanol solution eluate, concentrated the eluate under reduced pressure to an extract, and vacuum dried at 50°C to obtain Guangqian The extract of the total flavonoids of Herba Desmodium, adopt quality detection method of the present invention to carry out the quality detection of ...

Embodiment 2

[0039] Take the dried medicinal material of Desmodium mongolica and crush it into coarse powder 1kg, add 10kg of 70% ethanol for reflux extraction for 2 hours for the first time, add 8kg of 70% ethanol for 2.0 hours for the second extraction, filter, combine the filtrate, recover ethanol from the filtrate and concentrate under reduced pressure without alcohol concentrated to a thick paste with a relative density of 1.25 (60~70°C), take the above thick paste (1:4) and put it on the treated macroporous resin (D101) column, first elute with water until it is almost colorless, Then eluted with 10 times the amount of 30% ethanol solution, and finally eluted with 8 times the amount of 50% ethanol solution, collected the ethanol solution eluate, concentrated the eluate under reduced pressure to an extract, and vacuum dried at 50°C to obtain Guangqian The extract of the total flavonoids of Herba Desmodium, adopt the quality detection method of the present invention to carry out the qua...

Embodiment 3

[0041] Get the dry medicinal material of Desmodium spp. and grind it into coarse powder 1kg, add 10kg75% ethanol for reflux extraction for 2.5 hours for the first time, add 7.5kg volume concentration for the second time and extract with 75% ethanol for 1.6 hours, filter, combine the filtrates, recycle the ethanol from the filtrate and Concentrate under reduced pressure without alcohol smell, concentrate to a thick paste with a relative density of 1.25 (60~70°C), take the above thick paste (1:4) and put it on the treated macroporous resin (AB-8) column, first Elute with water until nearly colorless, then elute with 10 times the amount of 30% ethanol, and finally elute with 8 times the amount of 50% ethanol, collect the ethanol eluate, concentrate the eluate under reduced pressure to extract, and store at 60°C Vacuum drying promptly obtains the extract of the total flavonoids of Desmodium sativa, adopts the quality detection method of the present invention to carry out the qualit...

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Abstract

The invention relates to a quality detecting method for a traditional Chinese medicine extract, in particular to a quality detecting method for a desmodium styracifolium general flavone extract. The desmodium styracifolium general flavone extract is prepared through desmodium styracifolium smashing and alcohol or water extraction, ethyl alcohol recovery, concentration, macroporous adsorption resincolumn chromatography elution (ethyl alcohol-water system), concentration and vacuum drying. The preparation method of the desmodium styracifolium general flavone extract is reasonable, stable, shortin production period and suitable for industrial production. An ultraviolet-visible spectrophotometry method, a high efficiency liquid chromatography method and an area normalization method are adopted to determine the content of general flavone and schaftoside in the desmodium styracifolium general flavone extract, and the method is accurate, stable and simple in operation.

Description

technical field [0001] The invention relates to a quality detection method of a traditional Chinese medicine extract, in particular to a quality detection method of the total flavonoids extract of Desmodium sativa. Background technique [0002] Desmodium styracifolium (Osb.) Merr. is the dry aboveground part of the leguminous plant Desmodium styracifolium (Osb.) Merr. It is mainly distributed in Fujian, Hunan, Guangxi and Guangdong provinces. It has the effects of promoting dampness and reducing jaundice, diuresis and treating stranguria. It is clinically used for jaundice, red urine, hot stranguria, stone stranguria, astringent pain in urination, edema and oliguria. At present, the chemical constituents of Desmodium sativa ([Title] Study on the chemical constituents of Desmodium sativa [Author] Wang Zhirou, Bai Xianzhong, Liu Feng, Guangxi Medical University [Title] Journal of Guangxi Medical University, 1998(03): 10- 14. [Title] Isolation and Identification of Chemical Co...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
CPCG01N30/02G01N2030/027
Inventor 钟鸣冯军刘布鸣柴玲陈明生黄艳谭小青
Owner GUANGXI INST OF CHINESE MEDICINE & PHARMA SCI
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