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Culture medium containing blood plasma sediment and application thereof

A technology of sediment and culture medium, applied in the field of culture medium containing plasma sediment, can solve the problems of polluting the environment, wasting nutrient factor resources, etc., and achieve the effects of reducing production costs, improving economic benefits, energy saving and environmental protection, and social benefits

Pending Publication Date: 2018-02-13
JINAN JINNIU BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Animal serum is one of the application methods of animal blood. As the main raw and auxiliary materials for cell culture, animal serum has moved from the laboratory to the direction of professional and factory production; but after the serum is separated from animal blood, the remaining plasma sediment (The remaining blood clot after the blood is separated from the serum is called plasma sediment) is usually thrown away as industrial waste, which pollutes the environment and wastes a lot of nutritional factor resources

Method used

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  • Culture medium containing blood plasma sediment and application thereof
  • Culture medium containing blood plasma sediment and application thereof
  • Culture medium containing blood plasma sediment and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Embodiment 1: Cultivate Bacillus subtilis

[0018] (1) Strain selection: select Bacillus subtilis ZK-F3;

[0019] (2) Primary seed cultivation: inoculate the bacterial strain described in step (1) in 500ml LB liquid medium under aseptic conditions, cultivate at 37°C and 200rpm / min for 17h, and obtain the primary seed liquid;

[0020] (3) Secondary seed culture: with 2% inoculum size, the seed liquid is inoculated in the shake flask of the culture medium that contains plasma sediment, 37 ℃, 200rpm / min, fermentation 17h, shake flask fermentation finishes, obtains 1000ml secondary seed solution;

[0021] (4) fermentation tank fermentation culture:

[0022] ①Fermentation culture in 50L fermenter: Inoculate the seed solution in a fermenter (medium containing plasma sediment) with 2% inoculation amount, culture at 37°C, rotation speed 200rpm / min, ventilation rate 1:1.1, ferment 48h, fermentation finishes;

[0023] ② Fermentation culture in 2T fermenter: Inoculate the seed...

Embodiment 2

[0038] Embodiment 2: Cultivate Bacillus licheniformis

[0039] (1) Strain selection: select Bacillus licheniformis (Baxillus Licheniformis) ZK-F4;

[0040] (2) Primary seed cultivation: inoculate the bacterial strain described in step (1) in 500ml LB liquid medium under aseptic conditions, cultivate at 37°C and 200rpm / min for 17h, and obtain the primary seed liquid;

[0041] (3) Secondary seed culture: with 2% inoculum size, the seed liquid is inoculated in the shake flask of the culture medium that contains plasma sediment, 37 ℃, 200rpm / min, fermentation 17h, shake flask fermentation finishes, obtains 1000ml secondary seed solution;

[0042] (4) fermentation tank fermentation culture:

[0043] ①Fermentation culture in 50L fermenter: Inoculate the seed solution in a fermenter (medium containing plasma sediment) with 2% inoculation amount, culture at 37°C, rotation speed 200rpm / min, ventilation rate 1:1.1, ferment 48h, fermentation finishes;

[0044] ② Fermentation culture ...

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PUM

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Abstract

The invention discloses a culture medium containing blood plasma sediment. The culture medium is prepared from pre-treated blood plasma sediment, tryptone, yeast extract powder, sodium chloride and water, wherein the concentration of each component is as follows: 1g / L to 1.5g / L of the pre-treated blood plasma sediment, 2.5g / L to 3.5g / L of the tryptone, 1.2g / L to 1.8g / L of the yeast extract powderand 2.5g / L to 3.5g / L of the sodium chloride. The pre-treated blood plasma sediment is prepared by a method comprising the following steps: taking the blood plasma sediment; sterilizing, drying and crushing to obtain the pre-treated blood plasma sediment. The culture medium containing the blood plasma sediment, disclosed by the invention, can be used for culturing spore bacteria, especially bacillus subtilis, bacillus licheniformis and the like. The culture medium containing the blood plasma sediment, disclosed by the invention, has the advantages that 1, the utilization amount of expensive nutrient elements including the tryptone, the yeast extract powder, beef extract and the like can be replaced to a certain extent and the production cost is greatly reduced; 2, cyclic utilization of wastes is realized and green, energy-saving and environment-friendly social benefits are realized.

Description

technical field [0001] The invention relates to a culture medium containing plasma precipitate and its application. Background technique [0002] my country is a large breeding country and one of the countries with the richest animal blood resources in the world. According to statistics, about 20,000 tons of pig blood, 10,000 tons of cow blood, and 15,000 tons of sheep blood are produced every year. Currently, only a small part is used. , most of them are abandoned (especially large and medium cities). Vigorously developing and utilizing the animal blood resources in our country can obtain huge economic benefits. Animal serum is one of the application methods of animal blood. As the main raw and auxiliary materials for cell culture, animal serum has moved from the laboratory to the direction of professional and factory production; but after the serum is separated from animal blood, the remaining plasma sediment (The remaining blood clot after the blood is separated from the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12R1/07C12R1/145C12R1/01
CPCC12N1/20
Inventor 黄俊叶伟伟张龙王鈺鑫杨晓燕
Owner JINAN JINNIU BIOTECH CO LTD
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