Artificial auxiliary propagation method of cranes

Abstract

The invention discloses an artificial auxiliary propagation method of cranes. The artificial auxiliary propagation method comprises a newly-designed semen diluent formula of the cranes, a cold storageand defreezing technology of the semen of the cranes, and a judgment method of the semen delivery time of the cranes. By means of the artificial auxiliary propagation method of the cranes, the diluent formula which can store the semen of the cranes for a long time and an efficient and simple semen cold storage and defreezing method are designed, so that the vigor and the survival rate of sperms are obviously higher than those of an existing method.

Description

technical field [0001] The invention relates to an artificial assisted breeding method for cranes. Background technique [0002] The red-crowned crane (Gruus japonensis), also known as the crane, belongs to the order Gruiformes, the family Gruidae, and the genus Grus. Long beak, long neck, and long legs are the most obvious morphological characteristics of red-crowned cranes. The body length is 140-150cm, the body feathers are pure white, the secondary and tertiary flight feathers are black, and the skin on the top of the head is bare and bright red. It moves in shallow water wetlands. and foraging. After 5 months of age, the body shape and shape of young birds are similar to those of adults, but the feather tips are still slightly brown. The red-crowned crane is a national first-class protected bird. It is a typical migratory bird in my country and has been loved by people for thousands of years. It is a symbol of auspiciousness, longevity and happiness. The habitat of th...

AI Technical Summary

Problems solved by technology

However, there are still many technical obstacles in this method. One is that the amount of semen in cranes during the breeding period is low, and the amount of artificially collected crane semen that can be used for insemination is very limited, which makes artificial insemination technology difficult to popularize and apply; Second, the semen of cranes such as red-crowned cranes can only be collected and transfused immediately, and cannot be stored for a long time. It is difficult to meet the requirements of insemination by infusion now; the third is that the death rate of thawed crane sperm is relatively high, and dimethyl sulfoxide is mostly used as antifreeze for stored crane semen. If the concentration of antifreeze is not suitable, thaw If the method is incorrect, the vitality of the stored crane sperm will be seriously affected or even die, and the fertilized female crane will not get sperm with high vitality, which will affect the fertilization of eggs and the quality of embryos; the fourth is that the timing of insemination is not easy to judge , although the spawning time of female eggs has certain rules, it is difficult to judge the specific spawning time, especially the spawning time of each clutch and the next clutch or the same clutch, the first egg and the second egg, The storage time of the crane's semen in the fallopian tube of the female crane is not long. Generally speaking, the insemination time is the most suitable for the female crane to lay eggs for 3-4 days. The rest of the time is inseminated, and the chance of the female crane to lay fertilized eggs is less than 20%. Fifth, the female cranes consume a lot of nutrients during the breeding period. If the diet is not adjusted, the female cranes will not lay eggs, the eggs will be irregular, the amount of eggs will be small, and the quality of the eggs will be poor. It will be difficult to lay eggs in one breeding period. Progeny with high hatchling rate

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