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Preparation method of complement C3 detection kit

A detection kit and reagent technology, applied in the field of medical testing, can solve the problems of poor experimental stability, narrow linear range, and many interference factors, and achieve the effect of simple operation, wide linear range and good repeatability

Inactive Publication Date: 2018-02-23
ANHUI IPROCOM BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The introduction of microspheres can solve the problems of narrow linear range, many interference factors, and poor experimental stability in clinical testing to a certain extent.

Method used

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  • Preparation method of complement C3 detection kit
  • Preparation method of complement C3 detection kit
  • Preparation method of complement C3 detection kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] A preparation method of a complement C3 detection kit of the present embodiment comprises the following steps:

[0041] (1) Prepare reagent R1 according to the following component contents:

[0042] Reagent R1:

[0043]

[0044] The solvent is purified water;

[0045] ① According to the component content of the above reagent R1, dissolve Tris in purified water, stir evenly, wait until fully dissolved, and prepare R1 buffer solution;

[0046] ② Dissolve polyethylene glycol 8000, EDTA-Na, and sodium azide in R1 buffer according to the component content of the above reagent R1, stir well, and then adjust the pH to 8.5 with 1mol / L hydrochloric acid or sodium hydroxide, that is Prepare reagent R1;

[0047] (2) Prepare reagent R2 according to the following component contents:

[0048]

[0049] The solvent is purified water;

[0050] ① According to the component content of the above reagent R2, dissolve Tris in purified water, stir evenly, wait until fully dissolved, ...

Embodiment 2

[0058] A preparation method of a complement C3 detection kit of the present embodiment comprises the following steps:

[0059] (1) Prepare reagent R1 according to the following component contents:

[0060] Reagent R1:

[0061]

[0062] The solvent is purified water;

[0063] ① According to the component content of the above reagent R1, dissolve Tris in purified water, stir evenly, wait until fully dissolved, and prepare R1 buffer solution;

[0064] ② Dissolve polyethylene glycol 8000, EDTA-Na, and sodium azide in R1 buffer according to the component content of the above reagent R1, stir well, and then adjust the pH to 8.5 with 1mol / L hydrochloric acid or sodium hydroxide, that is Prepare reagent R1;

[0065] (2) Prepare reagent R2 according to the following component contents:

[0066] Reagent R2:

[0067]

[0068] The solvent is purified water;

[0069] ① According to the component content of the above reagent R2, dissolve Tris in purified water, stir evenly, wait...

Embodiment 3

[0077] A preparation method of a complement C3 detection kit of the present embodiment comprises the following steps:

[0078] (1) Prepare reagent R1 according to the following component contents:

[0079] Reagent R1:

[0080]

[0081] The solvent is purified water;

[0082] ① According to the component content of the above reagent R1, dissolve Tris in purified water, stir evenly, wait until fully dissolved, and prepare R1 buffer solution;

[0083] ② Dissolve polyethylene glycol 8000, EDTA-Na, and sodium azide in R1 buffer according to the component content of the above reagent R1, stir well, and then adjust the pH to 8.5 with 1mol / L hydrochloric acid or sodium hydroxide, that is Prepare reagent R1;

[0084] (2) Prepare reagent R2 according to the following component contents:

[0085] Reagent R2:

[0086]

[0087] The solvent is purified water;

[0088] ① According to the component content of the above reagent R2, dissolve Tris in purified water, stir evenly, wait...

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Abstract

The invention discloses a preparation method of a complement C3 detection kit, which comprises the following steps: (1) according to the component content of the reagent R1, the R1 buffer solution is prepared; polyethylene glycol 8000, EDTA-Na, sodium azide Dissolve in R1 buffer, and then adjust the pH to 8.5 with 1mol / L hydrochloric acid or sodium hydroxide to obtain reagent R1; (2) prepare R2 buffer according to the component content of reagent R2; BSA, EDTA‑Na, Tween‑ 20. Dissolve complexin, sucrose, NaCl, and sodium azide in R2 buffer to obtain R2 dispersion; prepare anti-human C3 antibody coated with polystyrene latex particles; dissolve polystyrene latex particles coated with R2 dispersion anti-human C3 antibody, and then adjust the pH to 7.4 with 1mol / L hydrochloric acid or sodium hydroxide to obtain reagent R2. The invention has the advantages that the prepared kit has high sensitivity and stability, and the operation is simple and fast.

Description

technical field [0001] The invention relates to the technical field of medical testing, in particular to a preparation method of a complement C3 detection kit. Background technique [0002] Complement C3 is composed of two peptide chains α and β linked by disulfide bonds. It is a β1 globulin with a molecular weight of 180kD and a sugar content of about 2.2%. It is the most abundant complement component in serum, accounting for about 1% of the total complement content. / 3 or more. In the process of complement system activation, whether it is the classical pathway or the alternative pathway, the activation of C3 is required to promote the subsequent chain reaction of complement components (C5-C9). Therefore, it plays a key role in both activation pathways. Complement C3 is mainly synthesized and secreted by hepatic parenchymal cells, and a small amount is synthesized by macrophages and monocytes. Under physiological conditions, the proteolytic enzymes present in body fluids...

Claims

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Application Information

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IPC IPC(8): G01N33/68G01N33/543G01N33/544
CPCG01N33/68G01N33/54313G01N33/544G01N2333/4716
Inventor 庄庆华朱卫中吴泽东张金东吴铮
Owner ANHUI IPROCOM BIOTECH CO LTD