A kind of recombinant Periplaneta americana thymosin protein pathy1 and its expression method
A technology of American cockroach and thymosin, applied in the field of genetic engineering, can solve the problems of difficult purification and low thymosin content
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[0029]Test material
[0030]1.1 America big sample
[0031]The American Damu sample is provided by the Sichuan Nice Doctor Panxi Pharmaceutical Co., Ltd. Xichang Farm Base.
[0032]1.2 plasmid vector and receptor strain
[0033]The gene prokaryotic expression vector PET28A is preserved for this laboratory. Escherichia coli DH5A, raw nuclear expression cells BL21 (DE3) were purchased from Bourway Co., Ltd.
[0034]1.3 restriction endonuclease and other enzymes
[0035]Restriction endonuclease (XHOI, ECOR I), T4DNA Ligase (DNA ligase), TAQ enzymes are available in Chengdu Tiantai (Takara) Bio Co., Ltd.
[0036]1.4 instrument equipment and consumables
[0037]UV spectrophotometer, PCR amplifier (Biometra, Germany), vertical pressure steam sterilization pot (Shanghai Boxing Industrial Co., Ltd.), protein vertical electric soot tank, transfer electrophoresis tank, shaker, oscillator, ultrasonic crusher, protein Purification instrument.
[0038]1.5 medium and solution
[0039]1.5.1 Liquid LB medium
[0040]100ml formula:...
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