Unlock instant, AI-driven research and patent intelligence for your innovation.

β-mannanase gene, recombinant expression vector, bacterial strain, β-mannanase and its preparation method and application

A technology of mannanase and expression vector, which is applied in the direction of microorganism-based methods, botany equipment and methods, biochemical equipment and methods, etc. It can solve the problems of low enzyme activity and poor high temperature resistance of β-mannanase , to achieve the effect of improving expression and broad application prospects

Active Publication Date: 2020-11-06
WUHAN POLYTECHNIC UNIVERSITY
View PDF12 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The main purpose of the present invention is to propose a kind of β-mannanase gene, recombinant expression vector, bacterial strain, β-mannanase and its preparation method and application, aiming to solve the resistance of β-mannanase in the prior art. Poor high temperature resistance and low enzyme activity

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • β-mannanase gene, recombinant expression vector, bacterial strain, β-mannanase and its preparation method and application
  • β-mannanase gene, recombinant expression vector, bacterial strain, β-mannanase and its preparation method and application
  • β-mannanase gene, recombinant expression vector, bacterial strain, β-mannanase and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0025] The invention provides a β-mannanase gene, a recombinant expression vector, a bacterial strain, a β-mannanase and its preparation method and application, aiming at solving the problem of poor high temperature resistance of β-mannanase in the prior art, live low problem.

[0026] Construction of expression vector of single copy β-mannanase gene

[0027] 1.1 Synthesis of gene fragments of β-mannanase

[0028] According to the existing β-mannanase gene (derived from Thermotoga maritima), artificially synthesized β-mannanase gene fragment, its nucleotide sequence is shown in SEQ ID NO:1 . The amino acid sequence corresponding to the artificially synthesized β-mannanase is shown in SEQ ID NO:2.

[0029] The gene sequence design of the artificially synthesized β-mannanase of the present invention mainly considers the following points:

[0030] 1) By replacing the original low-frequency codons with high-frequency codons in Pichia pastoris (P.pastoris);

[0031] 2) reduced...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a beta-mannase gene, a recombinant expression vector, a bacterial strain and beta-mannase as well as a preparation method and application thereof. A nucleotide sequence of thebeta-mannase gene is shown as SEQ ID NO. 1. The beta-mannase gene, the recombinant expression vector, the bacterial strain and the beta-mannase as well as the preparation method and the application thereof disclosed by the invention solve the problems of poor high temperature resistance and low enzyme activity of the beta-mannase in the prior art.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a β-mannanase gene, a recombinant expression vector, a bacterial strain, a β-mannanase and a preparation method and application thereof. Background technique [0002] β-mannanase has a very broad application prospect and is widely used in food, feed, health care, medicine, papermaking, textile and other industries. However, the β-mannanase in the prior art is not resistant to high temperature, but it is often accompanied by high temperature in industrial production and processing, such as spray drying of enzyme liquid, granulation and puffing of feed, etc. High temperature will occur. [0003] In the prior art, the gene of β-mannanase is generally amplified from related β-mannanase-producing microorganisms by PCR technology, connected to an expression vector, and then introduced into a relevant expression host to express β-mannanase enzyme. However, the β-mannanase obtained in this...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/56C12N9/24C12N15/81C12P19/02C12N1/19C12R1/84
CPCC12N9/2491C12N15/815C12N2800/22C12P19/02C12Y302/01025
Inventor 杨江科陈祺琛
Owner WUHAN POLYTECHNIC UNIVERSITY