Folic acid detection method based on near-infrared fluorescent probe copper nanoclusters
A technology of copper nano-clusters and fluorescent probes, which is applied in the field of biological analysis and detection, can solve problems such as interference with folic acid detection, vitamin C interference, and impact on folic acid determination, and achieve good biocompatibility, uniform size, and good water solubility.
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Embodiment 1
[0029] Synthesis and purification of embodiment 1 copper nanocluster
[0030] (1) Weigh 100mg ovalbumin and dissolve it in a beaker filled with 5mL high-purity water;
[0031] (2) Add 0.02 mL of 0.1M copper chloride solution to the solution obtained in step (1), and stir at room temperature for 10 min;
[0032] (3) Slowly inject 0.1mL 80% hydrazine hydrate (w / w) into the solution obtained in step (2), quickly replenish water to 8mL, and then stir at room temperature for 1h, until the solution turns pale yellow, indicating the formation of copper nanoclusters ;
[0033] (4) Purification: Purify the prepared copper nanoclusters with a dialysis bag with a molecular weight cut-off of 6000-8000 and a diameter of 25 mm, change the high-purity water every 4 hours, and perform dialysis for 24 hours. After the dialysis is completed, the purified copper nanoclusters are dried in vacuum at 35° C. Under the excitation of 340nm light, the fluorescent copper nanoclusters emitting in the n...
Embodiment 2
[0034] Synthesis and purification of embodiment 2 copper nanoclusters
[0035] (1) Weigh 100mg ovalbumin and dissolve it in a beaker filled with 5mL high-purity water;
[0036] (2) Add 0.10 mL of 0.1M copper chloride solution to the solution obtained in step (1), and stir at room temperature for 10 min;
[0037] (3) Slowly inject 0.5mL of 80% hydrazine hydrate (w / w) into the solution obtained in step (2), quickly replenish water to 15mL, and then stir at room temperature for 3h, until the solution appears light yellow, indicating the formation of copper nanoclusters ;
[0038] (4) Purification: Purify the prepared copper nanoclusters with a dialysis bag with a molecular weight cut-off of 6000-8000 and a diameter of 25 mm, change the high-purity water every 4 hours, and perform dialysis for 24 hours. After the dialysis is completed, the purified copper nanoclusters are dried in vacuum at 35° C. Under the excitation of 340nm light, the fluorescent copper nanoclusters emitting ...
Embodiment 3
[0039] Synthesis and purification of embodiment 3 copper nanoclusters
[0040] (1) Weigh 100mg ovalbumin and dissolve it in a beaker filled with 5mL high-purity water;
[0041] (2) Add 0.18 mL of 0.1M copper chloride solution to the solution obtained in step (1), and stir at room temperature for 10 min;
[0042] (3) Slowly inject 1.0mL of 80% hydrazine hydrate (w / w) into the solution obtained in step (2), quickly replenish water to 20mL, and then stir at room temperature for 4h, until the solution turns pale yellow, indicating the formation of copper nanoclusters ;
[0043] (4) Purification: Purify the prepared copper nanoclusters with a dialysis bag with a molecular weight cut-off of 6000-8000 and a diameter of 25 mm, change the high-purity water every 4 hours, and perform dialysis for 24 hours. After the dialysis is completed, the purified copper nanoclusters are dried in vacuum at 35° C. Under the excitation of 340nm light, the fluorescent copper nanoclusters emitting in ...
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