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New function of titanium dioxide nanoparticles in inhibiting growth of escherichia coli

A nanoparticle and Escherichia coli technology, which is applied in the direction of nanomedicine, nanotechnology, nanotechnology, etc., can solve the problem of low antibacterial properties of titanium dioxide nanoparticles, and achieve a significant antibacterial effect

Inactive Publication Date: 2018-03-16
安徽古尔特科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the biology, especially the antibacterial properties, of titanium dioxide nanoparticles are less involved.

Method used

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  • New function of titanium dioxide nanoparticles in inhibiting growth of escherichia coli

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Experimental program
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Effect test

Embodiment 1

[0019] Antibacterial activity detection of titanium dioxide nanoparticles:

[0020] The activated Escherichia coli was inoculated into a fresh medium at 2% and cultured for 4 hours to grow to the logarithmic growth phase, then 50 microliters of the bacterial solution was diluted 100 times and mixed with the medium and poured onto the plate. Insert the Oxford cup at the same time, add 150 microliters of positive control (ampicillin, 100 ng / microliter), negative control (distilled water), low concentration (20 micrograms of titanium dioxide nanoparticles), medium concentration (200 micrograms of titanium dioxide nanoparticles), For high concentration (2 mg nanoparticles) of the test substance, observe the existence of the inhibition zone after incubation for 16 hours.

Embodiment 2

[0022] Effect of titanium dioxide on target gene expression:

[0023] Titanium dioxide nanoparticles were added to the Escherichia coli liquid culture cultured to the above logarithmic growth phase so that the final concentration reached 50 mg / ml. After continuing to cultivate for 16 hours, the thalline was recovered, and the total mRNA in the cells was extracted using conventional technical means in the art, reverse-transcribed into cDNA, and the expression level of the OmpF gene was carried out using a Cycle480 (Roche) fluorescent quantitative PCR instrument (using SybrGreen dye). quantitative analysis. The PCR conditions used were: pre-denaturation at 95°C for 5 minutes, followed by 40 cycles of amplification at 95°C for 10s, 60°C for 10s, and 72°C for 30s. The primers used were: upstream CCAGGGTAACAACTCTGAAG; downstream GCCGCCAACACGACCAACGA. The obtained quantitative value is the relative ratio of the expression level of OmpF to the internal reference gene GAPDH. After ...

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Abstract

Nanoparticles have different properties from particles with the size of larger than micron, and one of the properties is inhibition of bacteria. The invention discloses a new function of titanium dioxide nanoparticles in inhibiting growth of escherichia coli. The new function is mainly shown by adding the titanium dioxide nanoparticles to inhibit the growth of the model microorganism escherichia coli and decrease the survival rate of the model microorganism escherichia coli, and a mechanism of the inhibition effect of the nanoparticles is shown by influencing the expression level of OmpF. Since the nanoparticles are used as materials widely, and through the discovery of the function, further understanding of the biological effect of the nanoparticles is facilitated.

Description

technical field [0001] The application belongs to the technical field of new functions of nanoparticles, and in particular relates to the new function of titanium dioxide nanoparticles for inhibiting the growth of Escherichia coli. Background technique [0002] Nanoparticles are artificially produced microscopic particles with a size no larger than 100 nanometers. Nanomaterials are of great value in science and application. In terms of biological effects, nanoparticles can often penetrate into membrane cells due to their small diameter and large surface area, thus producing completely different effects from particles above microns. . Titanium dioxide nanoparticles have very special optical properties, thermal stability, non-toxicity, super-hydrophilicity and other characteristics, and can be widely used in anti-ultraviolet materials, textiles, self-cleaning glass, sunscreen and other industries. However, the biology, especially the antibacterial properties, of titanium dio...

Claims

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Application Information

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IPC IPC(8): A01N59/16A01N25/12A01P1/00B82Y5/00
Inventor 高宁馨黄胜徐晓莉
Owner 安徽古尔特科技有限公司