Method for cultivating ganoderma lucidum by imitating wild conditions
A technology imitating wild cultivation and Ganoderma lucidum, applied in cultivation, plant cultivation, mushroom cultivation, etc., can solve the problems of insufficient active ingredients of Ganoderma lucidum, prone to excessive heavy metals, high production costs, etc., and achieve short production time, low cost, and work less effect
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Embodiment 1
[0017] The imitation wild cultivation method of this Ganoderma lucidum comprises the following steps:
[0018] A. Cultivate strains: Take 100L of distilled water, 2kg of agar, and 1 kg of glucose, stir evenly, and sterilize to prepare medium I. Select the fruiting bodies of wild Ganoderma lucidum as the mother species and cultivate them in medium cultivation I for 15 days, and control the temperature at 20°C. , to obtain the mother species; take 100 L of distilled water, 50 kg of maple wood bran, 4 kg of soybean meal, 4 kg of corn flour, 1 kg of glucose, 0.25 kg of superphosphate, and 0.25 kg of gypsum, stir evenly, and sterilize to prepare medium II, sterile Insert the mother seed and grow at 20°C for 45 days to obtain the original seed; take 100 L of distilled water, 100 kg of maple wood bran, 15 kg of soybean meal, 4 kg of corn flour, 1 kg of brown sugar, 0.5 kg of superphosphate, and 0.5 kg of gypsum and stir Evenly, sterilize to prepare the medium III, aseptically insert ...
Embodiment 2
[0023] This imitation wild cultivation method of Ganoderma lucidum, in step B, select 15-year-old maple wood, cut into sections about 10cm long, and then steam sterilize at a temperature of about 120°C for 5 hours, and then put it in a sterile room After cooling, it is ready for use; in step C, culture bacteria for 20 days in a dark environment at 27°C; other characteristics are the same as in Example 1.
Embodiment 3
[0025] This method of imitating wild cultivation of Ganoderma lucidum, in step B, select 10-year-old maple wood, cut into sections about 25cm long, and then steam sterilize at a temperature of about 100°C for 8 hours, and then put them in a sterile room After cooling, it is ready for use; in step C, culture bacteria for 30 days in a dark environment at 25°C; other characteristics are the same as in Example 1.
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