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Method for extracting hyaluronic acid from bovine testis

A hyaluronic acid and testis technology, applied in the field of hyaluronic acid extraction, can solve the problems of separation of bacteria and fermentation liquid, damage to human body and environment, high production cost, etc., and achieve the effect of short extraction time, simple ingredients and mild conditions

Inactive Publication Date: 2018-03-27
桂林华诺威生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the quaternary ammonium salt method is easy to precipitate the protein together when precipitating HA, reducing the purity of HA, and the amount of quaternary ammonium salt is relatively large; the chloroform extraction method is not only complicated in operation, but also causes damage to the human body and the environment; Due to the high viscosity of the acid, the high-speed centrifugation method is difficult to completely separate the bacteria from the fermentation liquid, and it is difficult to apply to industrial production; the flocculation method and the quaternary ammonium salt method also have the problems of high production cost and incomplete removal of impurities; the diameter of the pores of the microporous membrane is between Under the continuous action of pressure, it will become larger and cannot completely retain the bacteria and protein in the fermentation broth

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  • Method for extracting hyaluronic acid from bovine testis

Examples

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Comparison scheme
Effect test

Embodiment 1

[0022] A method for extracting hyaluronic acid from bovine testicles, specifically comprising the steps of:

[0023] 1) After peeling the fresh beef testicles, cut them into small pieces;

[0024] 2) Subcritical water extraction: put the processed raw materials in an extraction kettle, add sodium acetate equivalent to 0.010 parts by weight of raw materials, inject deoxygenated deionized water into the kettle, extract at a temperature of 120-130°C, and a pressure of 8-10MPa. The extraction time is 20 minutes, the bovine testis is extracted, the extraction is completed, the pressure is released, the extract flows into the collection tank and cooled to room temperature, and the extract is obtained;

[0025] 3) Add an equal volume of chloroform and n-butanol mixture to the extract obtained in the upward step, with a volume ratio of chloroform:n-butanol=4:1, stir evenly, and let stand;

[0026] 4) Alcohol precipitation: After standing and stratifying, take the supernatant and add ...

Embodiment 2

[0030] A method for extracting hyaluronic acid from bovine testicles, specifically comprising the steps of:

[0031] 1) After peeling the fresh beef testicles, cut them into small pieces;

[0032] 2) Subcritical water extraction: put the treated raw materials in an extraction kettle, add sodium acetate equivalent to 0.012 parts by weight of raw materials, inject deoxygenated deionized water into the kettle, extract at a temperature of 120-130°C, and a pressure of 8-10MPa. The extraction time is 25 minutes, and the bovine testis is extracted. After the extraction is completed, the pressure is released, and the extract flows into the collection tank and cooled to room temperature to obtain the extract;

[0033] 3) Add an equal volume of chloroform and n-butanol mixture to the extract obtained in the upward step, with a volume ratio of chloroform:n-butanol=4:1, stir evenly, and let stand;

[0034] 4) Alcohol precipitation: After standing and stratifying, take the supernatant and...

Embodiment 3

[0038] A method for extracting hyaluronic acid from bovine testicles, specifically comprising the steps of:

[0039] 1) After peeling the fresh beef testicles, cut them into small pieces;

[0040] 2) Subcritical water extraction: put the processed raw materials in an extraction kettle, add sodium acetate equivalent to 0.015 parts by weight of raw materials, inject deoxygenated deionized water into the kettle, extract at a temperature of 120-130°C, and a pressure of 8-10MPa. The extraction time is 30 minutes, the bovine testis is extracted, the extraction is completed, the pressure is released, the extract flows into the collection tank and cooled to room temperature, and the extract is obtained;

[0041] 3) Add an equal volume of chloroform and n-butanol mixture to the extract obtained in the upward step, with a volume ratio of chloroform:n-butanol=4:1, stir evenly, and let stand;

[0042] 4) Alcohol precipitation: After standing and stratifying, take the supernatant and add ...

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Abstract

The invention discloses a method for extracting hyaluronic acid from bovine testis. The method comprises the following steps of (1) after peeling fresh bovine testis, cutting into small pieces; (2) carrying out subcritical water extraction: putting the treated raw materials into an extraction kettle, adding sodium acetate, injecting deoxidized deionized water into the kettle, extracting the bovinetestis, decompressing after extracting, flowing an extract liquor into a collecting tank, and cooling to the room temperature to obtain an extraction solution, wherein the extraction temperature is 120 to 130 DEG C, the pressure is 8 to 10Mpa, and the extraction time is 20 to 30min; (3) adding isometric chloroform-normal butanol mixed liquor into the extraction solution obtained through the steps, uniformly stirring, and standing, wherein the volume ratio of chloroform to normal butanol is equal to 4:1; (4) carrying out alcohol precipitation; (5) drying to obtain a finished product.

Description

technical field [0001] The invention relates to an extraction method in the field of biotechnology, in particular to a method for extracting hyaluronic acid from bovine testes. Background technique [0002] Hyaluronic acid (HA for short) is an acidic polymucopolysaccharide, which is composed of N-acetylglucosamine and glucuronic acid repeatedly and alternately linked by β-1,4 and β-1,3 glycosidic bonds. . Due to its unique properties of high molecular polysaccharides such as water retention and lubrication, as well as natural, non-allergic and good biocompatibility with human tissues, it is widely used in the fields of cosmetics, health food and medicine. [0003] At present, hyaluronic acid is extracted from animal tissues mainly using cockscombs, human umbilical cords, animal eyeballs, etc. as raw materials, or produced by bacterial fermentation. The extraction and refining of hyaluronic acid brings great difficulties, and also reduces the quality of hyaluronic acid, lim...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/08
Inventor 韦忠明黄启斌
Owner 桂林华诺威生物科技有限公司
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