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Method with ultra-high pressure for extracting ganoderma lucidum polysaccharide in glossy ganoderma

An ultra-high pressure technology, a technology of Ganoderma lucidum polysaccharide, is applied in the field of Ganoderma lucidum polysaccharide extraction by ultra-high pressure technology, which can solve the problems of low extraction rate and low extraction purity, and achieve the effects of large adsorption capacity, good thermal stability and fast exchange speed.

Inactive Publication Date: 2018-03-30
DAXINGANLING LINGOBERRY BOREAL BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] The purpose of the present invention is to overcome the shortcomings of conventional techniques such as relatively low extraction rate and low extraction purity, and provide a method for extracting Ganoderma lucidum polysaccharides from Ganoderma lucidum using ultra-high pressure technology

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Embodiment 1: a kind of method utilizing ultra-high pressure technology to extract Ganoderma lucidum polysaccharide in Ganoderma lucidum, its steps are as follows:

[0025] (1) After the dry Ganoderma lucidum is crushed through a 60-mesh sieve, put it into a pressure-resistant bag, add 10 times the volume of ethanol with a concentration of 70%, and process it at an ultra-high pressure of 200MPA, 10min, and 18°C, and reclaim the ethanol at 60 ℃ vacuum drying to obtain the crude product;

[0026] (2) Add an appropriate amount of 75% ethanol to dissolve the crude ganoderma polysaccharide powder obtained, adjust the pH value to 5.5, heat to 60°C, add 2.0% pectinase (1 million u / ml), mix well and keep enzymatic hydrolysis for 0.5h . Cool to room temperature, let stand, filter, discard the filter residue, and concentrate the filtrate under reduced pressure to obtain crude ganoderma polysaccharide extract;

[0027] (3) Adsorb the obtained ganoderma lucidum polysaccharide cru...

Embodiment example 2

[0032] Implementation Case 2: A method for extracting Ganoderma lucidum polysaccharides from Ganoderma lucidum using ultra-high pressure technology, the steps are as follows:

[0033] (1) After the dry Ganoderma lucidum is crushed through an 80-mesh sieve, put it into a pressure-resistant bag, add 12 times the volume of ethanol with a concentration of 90%, process it at an ultra-high pressure of 300MPA, 15min, and 20°C, and reclaim the ethanol at 60 ℃ vacuum drying to obtain the crude product;

[0034] (2) Add an appropriate amount of 75% ethanol to dissolve the crude ganoderma polysaccharide powder obtained, adjust the pH value to 7.5, heat to 60°C, add 2.0% pectinase (1 million u / ml), mix well and keep enzymatic hydrolysis for 1.5h . Cool to room temperature, let stand, filter, discard the filter residue, and concentrate the filtrate under reduced pressure to obtain crude ganoderma polysaccharide extract;

[0035] (3) Adsorbing the obtained Ganoderma lucidum polysaccharide...

Embodiment example 3

[0040] Implementation Case 3: A method for extracting Ganoderma lucidum polysaccharides from Ganoderma lucidum using ultra-high pressure technology, the steps are as follows:

[0041] (1) After crushing the dried Ganoderma lucidum into a 80-mesh sieve, put it into a pressure-resistant bag, add 12 times the volume of ethanol with a concentration of 80%, and process it under ultra-high pressure 200MPA, 12min, 18°C, recover the ethanol, and heat it at 60°C The crude product was obtained by vacuum drying;

[0042] (2) Add an appropriate amount of 75% ethanol to dissolve the crude ganoderma polysaccharide powder obtained, adjust its pH value to 4.5, heat to 50°C, add 1.5% pectinase (1 million u / ml), mix well and maintain enzymatic hydrolysis for 2 hours. Cool to room temperature, let stand, filter, discard the filter residue, and concentrate the filtrate under reduced pressure to obtain crude ganoderma polysaccharide extract;

[0043] (3) Adsorbing the obtained Ganoderma lucidum p...

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PUM

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Abstract

The purpose of the present invention is to overcome the disadvantages of relatively low extraction rate and low extraction purity of conventional techniques, and provide a method for extracting polysaccharides from Ganoderma lucidum by using ultra-high pressure technology. The application of ultra-high pressure technology in the present invention is a treatment method that applies a pressure greater than 100 hydrostatic pressure to the raw material under normal temperature and near normal temperature conditions. The ultra-high pressure can make the solvent enter the interior of the plant cells, and the solvent can carry the active ingredient after the pressure is released. And overflow, to achieve high extraction efficiency of plant active ingredients, use macroporous resin to adsorb and purify Ganoderma lucidum polysaccharides, good adsorption selectivity to Ganoderma lucidum polysaccharides, fast adsorption and resolution, and large adsorption capacity.

Description

Technical field: [0001] The invention belongs to the field of natural organic chemistry and relates to a purification method of ganoderma lucidum polysaccharides, in particular to a method for extracting ganoderma lucidum polysaccharides from ganoderma lucidum by ultra-high pressure technology. Background technique: [0002] Ganoderma lucidum, also known as Lingzhi, grows best in the forest and has the highest medicinal effect. At present, there are also artificial greenhouses for cultivation, mainly growing in humid places. Ganoderma lucidum is a high-temperature fungus, which can grow between 15-35°C, and the optimum temperature is 25-30°C. Fruiting bodies can grow in the range of 10-32°C, but the optimum temperature for primordium differentiation and fruiting body development is 25-28°C. Below 25°C, the fruiting body grows slowly and the color of the shell is poor; above 35°C, the fruiting body will die. Ganoderma lucidum needs high humidity to grow. With the increase...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00
CPCC08B37/0003
Inventor 万莉姚德坤李玉伟苏学辉
Owner DAXINGANLING LINGOBERRY BOREAL BIOTECH CO LTD
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