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Set of serum markers for detection of aortic aneurysm/aortic dissection and application thereof

A technology for aortic dissection and aortic aneurysm, which is applied in the field of medicine and biology, and can solve problems such as unclear diagnosis of aortic aneurysm/aortic dissection

Inactive Publication Date: 2018-03-30
BEIJING INST OF HEART LUNG & BLOOD VESSEL DISEASES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, it is still unclear how to use these inflammatory factors to quickly and accurately diagnose aortic aneurysm / aortic dissection and their predictive role in disease development

Method used

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  • Set of serum markers for detection of aortic aneurysm/aortic dissection and application thereof
  • Set of serum markers for detection of aortic aneurysm/aortic dissection and application thereof
  • Set of serum markers for detection of aortic aneurysm/aortic dissection and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Example 1. Difference verification of aortic aneurysm / aortic dissection serological markers

[0040] Randomly grouped according to gender and age, 32 healthy people were selected as controls, and 31 patients with aortic aneurysm / aortic dissection were selected. The expression levels of protein factors in the extracted serum samples were detected using the QAH-CUST chip.

[0041] Experimental steps:

[0042] 1. Complete drying of the slide chip: Take the slide chip out of the box, and after equilibrating at room temperature for 20-30 minutes, open the packaging bag, uncover the sealing strip, and then put the chip in a vacuum desiccator or dry at room temperature for 1- 2 hours.

[0043] 2. Configuration of standard products:

[0044] (1) Add 500 μL of sample diluent to the vial of cytokine standard mixture and redissolve the standard. Before opening the vial, give it a quick centrifuge and gently pipette up and down to dissolve the powder. Label this vial as Std 1. ...

Embodiment 2

[0079] Example 2, large sample verification of aortic aneurysm / aortic dissection serological markers

[0080] Randomly grouped according to gender and age, 101 healthy people were selected as controls, 234 patients with aortic aneurysm / aortic dissection, and 203 patients with pulmonary embolism. An ELISA experiment of serum protein markers was carried out, and the corresponding RayBiotechHuman ELISA Kit was used to detect the expression levels of the nine protein factors obtained in Example 1, to verify whether the histone factors could effectively distinguish patients with aortic aneurysm / aortic dissection.

[0081] The experimental steps are as follows:

[0082] 1. Reagent preparation

[0083] (1) Equilibrate the kit and samples to room temperature (18-25°C);

[0084] (2) Samples According to the pre-test results, the serum samples of the persons to be tested were diluted according to the multiples shown in Table 2 below.

[0085] Table 2. Dilution multiples when detectin...

Embodiment 3

[0118] Embodiment 3, optimal diagnosis combination protein factor analysis

[0119] Through the ROC statistical analysis of the combined effect of each factor, it is determined to distinguish between aortic aneurysm / aortic dissection (group A) and healthy people (group H), pulmonary embolism (group PE) and healthy people (group H), aortic aneurysm / aortic dissection (group A) and healthy people (group H), pulmonary embolism (group PE) and healthy people (group H), Best diagnostic combination for aortic dissection (group A) and pulmonary embolism (group PE).

[0120] After further analysis, it was determined that the best combination of diagnostic factors for distinguishing aortic aneurysm / aortic dissection (group A) from healthy people (group H) was SAA+LCN2, and the serum of 423 healthy people and 418 cases of aortic aneurysm were selected again / Aortic dissection patient's serum, according to the method for embodiment 1-2, carry out serological marker detection, the result is...

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Abstract

The invention relates to a set of serum markers for detection of aortic aneurysm / aortic dissection and application thereof. The set of serum markers can be individually used for or arbitrarily combined for distinguishing aortic aneurysm / aortic dissection patients and normal people. The serum markers are protein factors, which include: LCN2: lipocalin, SAA: serum amyloid protein A.

Description

technical field [0001] The invention belongs to the technical field of medicine and biology, and specifically relates to a group of serum markers for detecting aortic aneurysm / aortic dissection and applications thereof. Background technique [0002] Aortic aneurysm / aortic dissection is a common critical cardiovascular disease, which is caused by local or diffuse abnormal dilation of the aortic wall and compression of surrounding organs, causing corresponding symptoms, and is a potentially fatal disease. At present, the diagnosis of aortic aneurysm / aortic dissection mainly relies on imaging examination, but it lacks convenience. Therefore, using effective laboratory tests to diagnose, prevent and predict aortic aneurysm / aortic dissection has become a very active research field, and more importantly, monitor and predict the evolution of the disease over time and assess clinical risk. [0003] Currently the most commonly used biomarker is D-dimer (D-dimer), the sensitivity can...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68
CPCG01N33/6893G01N2800/329
Inventor 杜杰李玉琳杨新颖芦洁
Owner BEIJING INST OF HEART LUNG & BLOOD VESSEL DISEASES
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