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Preparation method of high-embedding-rate small-particle-size euphausia superba oil nano-particles

An Antarctic krill oil and small particle size technology, which is applied in food forming, food ingredient function, food homogenization, etc., can solve the problems of large particle size, low embedding rate, and uneven particle size , to achieve the effect of reducing the particle size of nanoparticles, convenient operation, simple and reliable method

Active Publication Date: 2018-04-06
DALIAN POLYTECHNIC UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Through preliminary investigations, it was found that in the process of preparing nanoparticles, the predecessors initially mixed the oil phase and the water phase to obtain a coarse emulsion, and then directly carried out high-pressure homogenization under a certain pressure to obtain a nanoemulsion, resulting in the existence of a particle size of the prepared nanoparticles. Inhomogeneity, low embedding rate, large particle size, etc.

Method used

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  • Preparation method of high-embedding-rate small-particle-size euphausia superba oil nano-particles
  • Preparation method of high-embedding-rate small-particle-size euphausia superba oil nano-particles
  • Preparation method of high-embedding-rate small-particle-size euphausia superba oil nano-particles

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Preparation of coarse emulsifier

[0031] Dissolve 0.3 g of Antarctic krill oil into 20 ml of dichloromethane and acetone solution at a ratio of 1:2 as the oil phase. Dissolve the stabilizer (sodium caseinate, gum arabic and Tween-20) into the phosphate buffer solution with pH=7, the concentration of the stabilizer in the aqueous phase is 2% to 3%, and the adding temperature is 30°C to 60 ℃, stirring time 0.5 ~ 1h. The mixing ratio of the oil phase and the water phase is 1:9, pour the oil phase into the water phase, and mix the oil phase and the water phase evenly with a magnetic stirrer and a homogenizer.

[0032] High pressure homogenization

[0033] The uniformly mixed emulsion is subjected to high-pressure homogenization, and the homogenization force is 0-50 bar for one cycle. Then the nanoemulsion was subjected to rotary evaporation under vacuum to remove the organic solvent. The obtained nanoemulsion is subjected to vacuum freeze-drying to obtain nanoparticles...

Embodiment 2

[0043] Preparation of coarse emulsifier

[0044]Dissolve 0.3 g of Antarctic krill oil into 20 ml of dichloromethane and acetone solution at a ratio of 1:2 as the oil phase. Dissolve the stabilizer (sodium caseinate, gum arabic and Tween-20) into the phosphate buffer solution with pH=7, the concentration of the stabilizer in the aqueous phase is 2% to 3%, and the adding temperature is 30°C to 60 ℃, stirring time 0.5 ~ 1h. The mixing ratio of the oil phase and the water phase is 1:9, pour the oil phase into the water phase, and mix the oil phase and the water phase evenly with a magnetic stirrer and a homogenizer.

[0045] High pressure homogenization

[0046] The uniformly mixed emulsion is subjected to high-pressure homogenization, and the homogenization pressure is 200-400 bar for one cycle. Then the nanoemulsion was subjected to rotary evaporation under vacuum to remove the organic solvent. The obtained nanoemulsion is subjected to vacuum freeze-drying to obtain nanopart...

Embodiment 3

[0056] Preparation of coarse emulsifier

[0057] Dissolve 0.3 g of Antarctic krill oil into 20 ml of dichloromethane and acetone solution at a ratio of 1:2 as the oil phase. Dissolve the stabilizer (sodium caseinate, gum arabic and Tween-20) into the phosphate buffer solution with pH=7, the concentration of the stabilizer in the aqueous phase is 2% to 3%, and the adding temperature is 30°C to 60 ℃, stirring time 0.5 ~ 1h. The mixing ratio of the oil phase and the water phase is 1:9, pour the oil phase into the water phase, and mix the oil phase and the water phase evenly with a magnetic stirrer and a homogenizer.

[0058] High pressure homogenization

[0059] The uniformly mixed emulsion is subjected to high-pressure homogenization, and the homogenization pressure is 550-650 bar for one cycle. Then the nanoemulsion was subjected to rotary evaporation under vacuum to remove the organic solvent. The obtained nanoemulsion is subjected to vacuum freeze-drying to obtain nanopar...

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Abstract

The invention provides a preparation method of high-embedding-rate small-particle-size euphausia superba oil nano-particles. A specific technical scheme of the preparation method of the high-embedding-rate small-particle-size euphausia superba oil nano-particles comprises the following steps of taking a mixture of sodium caseinate, Arabia gum and twain-20 as a stabilizer, and dissolving the stabilizer in a phosphate buffer solution so as to obtain an aqueous phase; dissolving euphausia superba oil in mixed solvents of dichloromethane and acetone so as to obtain an oil phase; adding the oil phase into the aqueous phase so as to obtain a crude nanoscale emulsion; homogenating the crude nanoscale emulsion, carrying out high-pressure homogenization, and removing the organic solvents from the emulsion in a vacuum state so as to obtain a nano-emulsion; and then, performing vacuum drying on the nano-emulsion so as to obtain the high-embedding-rate small-particle-size euphausia superba oil nano-particles. By regulating cyclic pressure during the high-pressure homogenization process of the emulsion, morphological uniformity of the euphausia superba oil nano-particles is improved; and thus,particle size of the nano-particles is reduced.

Description

technical field [0001] The invention relates to the field of preparation of Antarctic krill oil nanoparticles, more specifically, relates to a method for preparing Antarctic krill oil nanoparticles with high uniformity, embedding rate and low nanoparticle size. Background technique [0002] Antarctic krill is the largest single species of biological resource on earth, with huge reserves. Antarctic krill oil is mainly composed of glycerides, phospholipids, free fatty acids, sterols and astaxanthin. Antarctic krill oil is rich in phospholipid-type Omega-3 polyunsaturated fatty acids and astaxanthin, giving it a variety of biological activities, but the oxidation stability of phospholipid-type polyunsaturated fatty acids is significantly lower than that of neutral glycerides and sterols. Grease; free fatty acids have a pro-oxidative effect, which can reduce the oxidative stability of other coexisting lipid components and accelerate the deterioration of grease; and phospholipid...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L33/115A23L5/00A23P10/30
CPCA23V2002/00A23L5/00A23L33/115A23P10/30A23V2200/222A23V2200/30A23V2250/5028A23V2300/26
Inventor 宋亮周大勇张建润张晶栗冰莹王枭
Owner DALIAN POLYTECHNIC UNIVERSITY