Application of hederagenin and its glucoside in preparation of medicine for treating cerebral apoplexy
A technology for helexin and cerebral apoplexy, which is applied in the field of medicine and can solve the problems such as helexin and its glycosides that have not yet been seen
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Embodiment 1
[0042] Embodiment 1: The pharmacological action of helexin and its glycosides
[0043] 1. Effect on survival time of mice after cerebral ischemia induced by bilateral ligation of common carotid artery and vagus nerve
[0044] 160 Kunming mice were randomly divided into 16 groups according to body weight and sex: model group and hedera saponin or saponin administration group with serial number 1-15. The mice in the treatment group were given 50 mg / kg / d of helexin or saponin by intragastric administration, and the mice in the model group were given an equal volume of 0.5% CMC-Na by intragastric administration for 2 consecutive weeks. 0.5 hours after the last gavage, the mice were superficially anesthetized with ether, and the bilateral common carotid arteries and vagus nerves were quickly ligated. The survival time of the mice was observed and recorded, and the differences in the survival time of the mice in each group were compared.
[0045]Experimental results: the survival t...
Embodiment 2
[0052] Embodiment 2: Preparation and pharmacological action of an extract containing helexin or its glycosides
[0053] Part 1: Preparation of Extract
[0054] Preparation method one 5kg of dry Lonicera chinense medicinal material, reflux extraction with 70% ethanol for 3 times, 2h each time, filter, combine the filtrate, reclaim the solvent under reduced pressure to obtain extractum (get partly dried to obtain total extract 1), add appropriate amount of water to suspend , filter, and load the sample on D101 macroporous resin, start gradient elution from 10%, 30%, 50%, 70%, 90% ethanol, and monitor whether the eluate contains Decaisoside E, Honeysuckle saponin B or Dipsacus saponin B, any of the three appear in the eluent and start to collect until all three are eluted from the resin and stop collecting. Concentrate and dry this part of the eluent to obtain the extract of Lonicera tomentosa Object 1.
[0055] Preparation method two 5kg of dry Lonicera chinensis medicinal ...
Embodiment 3
[0075] Embodiment 3: pharmaceutical preparation
[0076]1. Tablet: 5g of helexin or saponin or Lonicera tomentosa extract of serial number 1-15, 50g of starch, 3g of magnesium stearate. Preparation process: take the hedera saponin or saponin or Lonicera tomentosa extract of serial number 1-15, add starch and magnesium stearate, mix evenly, make granules, dry, and compress into tablets.
[0077] 2. Capsules: 5g of hedera saponin or saponin or Lonicera tomentosa extract with serial number 1-15, 50g of starch, 3g of magnesium stearate. Preparation process: take the hedera saponin or saponin or Lonicera chrysanthemum extract of serial number 1-15, add starch and magnesium stearate, mix evenly, make granules, dry, and pack into capsules.
[0078] 3. Injection: 1g of hedera saponin or saponin or Lonicera tomentosa extract with serial number 1-15, appropriate amount of sodium chloride for injection. Preparation process: take the hedera saponin or saponin or Lonicera tomentosa extra...
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