Pilot plant test type automatic synthesis instrument for nucleic acid DNA/RNA
An automatic synthesis, nucleic acid technology, applied in chemical instruments and methods, sustainable manufacturing/processing, preparation of sugar derivatives, etc., can solve the problems of uncontrollable reaction temperature, low reaction yield, and high cost, and achieve low use cost, Good stability and easy maintenance
Active Publication Date: 2018-04-06
CS BIO SHANGHAI
View PDF7 Cites 1 Cited by
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
[0003] The purpose of the present invention is to provide a pilot-type automatic synthesizer for nucleic acid DNA / RNA, which mainly solves the technical problems that the existing synthesizer cannot control the reaction temperature, resulting in low reaction yield, high energy consumption and high cost.
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View moreImage
Smart Image Click on the blue labels to locate them in the text.
Smart ImageViewing Examples
Examples
Experimental program
Comparison scheme
Effect test
Embodiment Construction
[0017] refer to Figure 1-9 , an automatic synthesizer for nucleic acid DNA / RNA pilot test type, comprising a first path and a second path, the first path includes 12 branches, and the 12 branches are provided with a twelve-way solenoid valve 45, which is washed by acetonitrile Solvent liquid path a1, acetonitrile washing solvent liquid path b2, adenine liquid path 3, cytosine liquid path 4, guanine liquid path 5, thymine liquid path 6, inert gas path 7, activator liquid path 8, base liquid road a9, base liquid road b10, base liquid road c11 and base liquid road d12, the second passage includes 12 branch roads, 12 branch roads are provided with twelve-way solenoid valve 45, which is washed by acetonitrile solvent liquid road c13, acetonitrile washing solvent liquid path d14, spare solution liquid path a15, spare solution liquid path b16, cap liquid liquid path a17, lye liquid path for activation b18, inert gas path 19, spare solution liquid path c20, spare solution liquid path...
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More PUM
Login to View More Abstract
The invention discloses a pilot plant test type automatic synthesis instrument for nucleic acid DNA / RNA, and mainly solves the problems of low efficiency, high energy consumption and high cost of theprior synthesis instrument. The pilot plant test type automatic synthesis instrument comprises a first access and a second access, the output pipelines of the first access and the second access are connected with a metering pump a through two two-way electromagnetic valves respectively, the output ends of the first access and the second access are connected with a two-way electromagnetic valve group a and then connected with a premix tank through pipelines, the premix tank is externally connected with a three-way electromagnetic valve, the output end of the two-way electromagnetic valve groupa is divided into two ways and respectively connected with a two-way electromagnetic valve group b and a two-way electromagnetic valve group c through a two-way electromagnetic valve c, and the firstelectromagnetic valve of the two-way electromagnetic valve group b is connected with a double-layer transparent reaction container with a clamping sleeve; the double-layer transparent reaction container is provided with a circulating water bath entrance and a circulating water bath exit as well as a piston pneumatic valve on the upper portion, the output end of the two-way electromagnetic valve group b located above the double-layer transparent reaction container is connected with a three-way electromagnetic valve group, the output end of the three-way electromagnetic valve group is connectedwith a metering pump b, and the other end of the metering pump b is located at the output end of the two-way electromagnetic valve group c under the double-layer transparent reaction container with the clamping sleeve. The pilot plant test type automatic synthesis instrument is used for pilot plant test automatic production of the nucleic acid DNA / RNA.
Description
technical field [0001] The invention relates to Oligo (nucleic acid) DNA / RNA solid-phase synthesis in the field of biotechnology, in particular to an automatic synthesizer for pilot synthesis of Oligo DNA / RNA. Background technique [0002] Oligo synthesis uses the solid-phase phosphoramidite triester method to synthesize oligonucleotide fragments with specific known sequences. This method has the characteristics of high efficiency, fast coupling and relatively stable starting reactants. It can be used for directional modification of gene sequences and is widely used in the field of life sciences. Its role in new energy, new materials, artificial life, nucleic acid vaccines, biomedicine and other fields has been initially reflected. Oligo DNA / RNA synthesis involves deprotection, coupling, washing, capping, oxidation, and other steps. After the above five steps, a nucleotide is connected to the nucleotide of the solid-phase carrier. Repeat the above steps until all the bases ...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More Application Information
Patent Timeline
Login to View More Patent Type & Authority Applications(China)
IPC IPC(8): C07H21/02C07H21/04C07H1/00
CPCC07H1/00C07H21/02C07H21/04Y02P20/10
Inventor 张恒维巢庆
Owner CS BIO SHANGHAI