An automatic synthesizer for nucleic acid dna/rna production
A technology for automatic synthesis and nucleic acid, applied in biochemical instruments, enzymology/microbiology devices, bioreactor/fermenter combinations, etc., can solve the problems of high energy consumption, low yield, high cost, etc., and achieve good stability, Low cost of use and easy maintenance
Active Publication Date: 2021-04-09
CS BIO SHANGHAI
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[0003] The purpose of the present invention is to provide an automatic synthesizer for nucleic acid DNA / RNA production, to solve the automatic synthesis of DNA / RNA industrial production, and to solve the low output in the prior art by controlling the reaction temperature, circulating the reaction solution, and overhead stirring technology , low yield, high energy consumption and high cost technical problems
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[0016] refer to Figure 1-8 , an automatic synthesizer for nucleic acid DNA / RNA production type, comprising a first path and a second path, the first path includes 12 branches, and the 12 branches are provided with a twelve-way solenoid valve 46, washed by acetonitrile solvent Liquid path a1, acetonitrile washing solvent liquid path b2, adenine liquid path 3, cytosine liquid path 4, guanine liquid path 5, thymine liquid path 6, inert gas path 7, activator liquid path 8, base liquid path a9, base liquid path b10, base liquid path c11 and base liquid path d12 are composed, the second path includes 12 branches, 12 branches are provided with 12-way electromagnetic valve 46, washed by acetonitrile solvent liquid path c13 , acetonitrile washing solvent liquid path d14, spare solution liquid path a15, spare solution liquid path b16, cap liquid liquid path a17, lye liquid path for activation b18, inert gas path 19, spare solution liquid path c20, spare solution liquid path d21, The o...
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The invention discloses an automatic synthesizer for nucleic acid DNA / RNA production, which mainly solves the technical problems of low yield, high energy consumption and high cost of the existing synthesizer. The present invention includes a first passage and a second passage, the output pipelines of which are respectively connected to the metering pump a through two two-way solenoid valves, and the output end is connected to the two-way solenoid valve group a, and then connected to the premixing tank through the pipeline. The mixing tank is externally connected with a three-way solenoid valve a, and the output end of the two-way solenoid valve group a is divided into two circuits through the two-way solenoid valve c to connect the two-way solenoid valve group b and the two-way solenoid valve group c respectively. The first solenoid valve is connected to the jacketed reaction kettle through the pipeline, and it is connected with a three-way solenoid valve b externally. It is equipped with a circulating water bath inlet and a circulating water bath outlet and a built-in mixer. The output end of the two-way solenoid valve group b above it is connected to a three-way Solenoid valve group, the output end of the three-way solenoid valve group is connected to the metering pump b, and the other end of the metering pump b is connected to the output port of the second solenoid valve group c located under the jacketed reactor. The invention is used for the industrialized production of nucleic acid DNA / RNA.
Description
technical field [0001] The invention relates to nucleic acid DNA / RNA solid-phase synthesis in the field of biotechnology, in particular to an automatic synthesizer for nucleic acid DNA / RNA production. Background technique [0002] Nucleic acid (Oligo) synthesis is to use the solid-phase phosphoramidite triester method to synthesize oligonucleotide fragments with specific known sequences. This method has the characteristics of high efficiency, fast coupling and relatively stable starting reactants. It can be used for directional modification of gene sequences and is widely used in the field of life sciences. Its role in new energy, new materials, artificial life, nucleic acid vaccines, biomedicine and other fields has been initially demonstrated. Oligo DNA / RNA synthesis involves deprotection, coupling, washing, capping, oxidation, and other steps. After the above five steps, a nucleotide is connected to the nucleotide of the solid-phase carrier. Repeat the above steps until ...
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IPC IPC(8): C12M1/38C12M1/02C12M1/00C07H1/00C07H21/02C07H21/04
CPCC07H1/00C07H21/02C07H21/04C12P19/34Y02P20/10
Inventor 张恒维巢庆
Owner CS BIO SHANGHAI