Method for simultaneously determining aflatoxins and flavoring agents in vegetable oil based on liquid-liquid extraction-liquid chromatography-tandem mass spectrometry

A technology of aflatoxin and liquid chromatography, applied in the field of simultaneous determination of aflatoxins and flavoring agents in vegetable oils based on liquid-liquid extraction-liquid chromatography-tandem mass spectrometry, achieving good reproducibility and less use of samples and solvents , The effect of meeting the needs of daily testing work

Inactive Publication Date: 2018-04-13
DALIAN UNIV OF TECH
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  • Claims
  • Application Information

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Problems solved by technology

At present, the methods for simultaneous detection of 6 aflatoxins AFB1, AFB2, AFG1, AFG2, AFM1, AFM2; 3 fl

Method used

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  • Method for simultaneously determining aflatoxins and flavoring agents in vegetable oil based on liquid-liquid extraction-liquid chromatography-tandem mass spectrometry
  • Method for simultaneously determining aflatoxins and flavoring agents in vegetable oil based on liquid-liquid extraction-liquid chromatography-tandem mass spectrometry
  • Method for simultaneously determining aflatoxins and flavoring agents in vegetable oil based on liquid-liquid extraction-liquid chromatography-tandem mass spectrometry

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Embodiment 1

[0064] (1) Liquid-liquid extraction: Weigh 1g of sample, add 2mL of acetonitrile, vortex for 30s, ultrasonicate at 20-25°C for 10min, ultrasonic frequency is 35Khz, centrifuge at 10,000r / min for 5min, then take 1mL of supernatant to be purified.

[0065] (2) Purification by liquid-liquid distribution: Add 2 mL of n-hexane to 1 mL of the supernatant, vortex for 30 s, centrifuge at 10,000 r / min for 1 min, remove the lower layer, pass through a microporous membrane, and wait for analysis.

[0066] (3) Standard solution preparation: First, accurately weigh 0.1 g (accurate to 0.0001 g) of vanillin, methyl vanillin and ethyl vanillin, dissolve in methanol and dilute to a 100 mL volumetric flask to make 1.0 mg / mL standard stock solution, diluted with acetonitrile to 1.0mg / L mixed standard stock solution 1. Absorb appropriate amount of aflatoxin AFB1, AFB2, AFG1, AFG2, AFM1 and AFM2 standard solutions and dilute to 10mL volumetric flask to prepare 0.1mg / L mixed standard stock solutio...

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Abstract

The invention discloses a method for simultaneously determining aflatoxins and flavoring agents in vegetable oil based on liquid-liquid extraction-liquid chromatography-tandem mass spectrometry. The method can simultaneously determine aflatoxin B1, aflatoxin B2, aflatoxin G1, aflatoxin G2, aflatoxin M1, aflatoxin M2, vanillin, methyl vanillin and ethyl vanillin in the vegetable oil. The method comprises the following steps: carrying out extraction on a sample through using an acetonitrile solvent, carrying out ultrasonically assisted extraction, purifying the obtained extraction solution by n-hexane, performing analysis under gradient conditions, carrying out qualitative analysis through comparing target analytes in retention time and ion pair information in a multi-reaction monitoring manner, and carrying out external standard method quantification. The method has the advantages of small amounts of the sample and reagents, fast analysis speed, high sensitivity, good reappearance and realization of simultaneous detection of aflatoxins and commonly used flavoring agents, and provides a new detection method for evaluating the quality and the safety of vegetable oil samples.

Description

technical field [0001] The invention relates to a method for simultaneously determining six kinds of aflatoxins in vegetable oils, aflatoxin B1 (AFB1), aflatoxin B2 (AFB2), aflatoxin G1 (AFG1), aflatoxin G2 (AFG2), aflatoxin Toxin M1 (AFM1) and aflatoxin M2 (AFM2); methods for three flavoring agents vanillin, methyl vanillin and ethyl vanillin, belonging to the technical field of food monitoring, specifically related to liquid-liquid extraction-based Liquid chromatography-tandem mass spectrometry positive ion mode determination method. Background technique [0002] Edible vegetable oils include rapeseed oil, soybean oil, corn oil, peanut oil, olive oil, sesame oil, sunflower oil, etc., which are necessities in daily life. Human beings consume a certain amount of vegetable oil in different forms every day. With the modern living standard With the improvement of food consumption, people not only have higher and higher requirements on the quality, nutrition and taste of vegeta...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06
Inventor 赵洪霞曲宝成姜菁秋王琰
Owner DALIAN UNIV OF TECH
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