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High-recovery rate dextranum separating and purifying method

A technology with high recovery rate of dextran, which is applied in the field of separation and purification of dextran with high recovery rate, can solve problems such as difficulties, and achieve the effects of increasing yield, facilitating widespread popularization and use, and great economic benefits

Inactive Publication Date: 2018-04-20
河北赛诺膜技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Aiming at the deficiencies in the prior art, the present invention provides a method for separating and purifying dextran with a high recovery rate, which solves the difficult problems raised in the background technology

Method used

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  • High-recovery rate dextranum separating and purifying method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Take 300kg of fermented liquid after high-temperature sterilization, with a sugar content of 10.5Bx, and filter it with a 5 μm plate frame and a filter cloth filter bag. The filtered fermented liquid is different from the first-stage ultrafiltration system with a molecular weight cut-off of 150,000Da. liquid and retentate. Among them, the permeable liquid is 340kg, and the concentrated liquid is 60kg. The permeate passes through a secondary ultrafiltration system with a molecular weight cut-off of 5000Da to remove fructose, and the retentate of the secondary ultrafiltration enters the molecular weight degradation, adds acid and heats for hydrolysis, hydrolyzes at 80°C for 4 hours, and then adds alkali to neutralize to a pH of 4. The circulating water is cooled to 40°C, and then the three-stage ultrafiltration system with a molecular weight cut-off of 5000Da is continuously added for dialysis to obtain a retentate with a conductivity of 1000us / cm, which is then decoloriz...

Embodiment 2

[0023] Take 300kg of fermented liquid after high-temperature sterilization, with a sugar content of 10.5Bx, and filter it with a 5 μm plate frame and a filter cloth filter bag. liquid and retentate. Among them, the permeable liquid is 320kg, and the concentrated liquid is 80kg. The permeate passes through a secondary ultrafiltration system with a molecular weight cut-off of 5000Da to remove fructose, and the retentate of the secondary ultrafiltration enters the molecular weight degradation, adds acid and heats for hydrolysis, hydrolyzes at 80°C for 4 hours, and then adds alkali to neutralize to a pH of 4. The circulating water is cooled to 40°C, and then the three-stage ultrafiltration system with a molecular weight cut-off of 5000Da is continuously added for dialysis to obtain a retentate with a conductivity of 1000us / cm, which is then decolorized by activated carbon, and then enters the drying section after removing the activated carbon with a plate and frame. The obtained ...

Embodiment 3

[0025] Take 300kg of fermented liquid after high-temperature sterilization, with a sugar content of 10.5Bx, and filter it with a 5 μm plate frame and a filter cloth filter bag. The filtered fermented liquid is different from the primary ultrafiltration system with a molecular weight cut-off of 100,000 Da. liquid and retentate. Among them, the permeable liquid is 320kg, and the concentrated liquid is 80kg. The permeate passes through a secondary ultrafiltration system with a molecular weight cut-off of 1000Da to remove fructose, and the retentate of the secondary ultrafiltration enters the molecular weight degradation, adds acid and heats for hydrolysis, and hydrolyzes at 80°C for 4 hours, then adds alkali to neutralize to pH 4, using The circulating water is cooled to 40°C, and then the three-stage ultrafiltration system with a molecular weight cut-off of 1000Da is continuously added for dialysis to obtain a retentate with a conductivity of 1000us / cm, and then enters the activ...

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Abstract

The invention discloses a high-recovery rate dextranum separating and purifying method. The high-recovery rate dextranum separating and purifying method comprises the following steps: step one, pretreatment: carrying out coarse filtration on a fermented solution, and removing large-particle impurities; step two, first-stage ultrafiltration: adding pure water for dialyzing during an ultrafiltrationprocess, thus obtaining a transparent solution and a trapped solution; enabling the transparent solution and the trapped solution to respectively enter different procedures; step three, second-stageultrafiltration: carrying out the second-stage ultrafiltration which the filter accuracy is higher on the transparent solution obtained through the first-stage ultrafiltration, and removing fructose;carrying out molecular weight degradation treatment on the obtained trapped solution, adding alkali for neutralizing, and cooling, thus obtaining dextranum hydrolysate; step four, third-stage ultrafiltration: carrying out the three-stage ultrafiltration on cooled hydrolysate, and removing ions; carrying out activated carbon decoloration on the obtained trapped solution, and then drying, thus obtaining a finished product; step five, recycling of first-stage ultrafiltration trapped solution, carrying out molecular weight degradation on the trapped solution, and then sequentially carrying out thesteps one, two, three and four. The technology is capable of recycling and reutilizing the trapped solution of the first-stage ultrafiltration system, and the recovery rate of dextranum is effectively increased.

Description

technical field [0001] The invention relates to the technical field of dextran separation and purification methods, in particular to a high recovery rate dextran separation and purification method. Background technique [0002] Dextran (Dextran) uses Leuconostoc intestinal mucosa as the strain, uses sucrose as the substrate, and decomposes the sucrose through the catalysis of enzymes, and at the same time, the glucose is polymerized to form dextran (dextran). The high-molecular dextran produced in the fermentation broth is extracted by alcohol precipitation, and then undergoes molecular weight degradation treatment to obtain thousands to hundreds of thousands of dextran molecules with different degrees of polymerization. The "Chinese Pharmacopoeia" includes several varieties of dextran 70, dextran 40, and dextran 20, with molecular weights of about 70,000, 40,000, and 20,000 Da, respectively. [0003] Ultrafiltration has the characteristics of high efficiency, low energy co...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/02
CPCC08B37/0003C08B37/0021
Inventor 林亚凯李焕霞赵杰何柳东宋玉萍
Owner 河北赛诺膜技术有限公司
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