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Extraction method for plant condensed tannin high polymers

A technology of condensed tannin and extraction method, which is applied in the field of extraction and purification of plant tannin, can solve the problems of research and application limitations, difficulty of condensed tannin, etc., and achieve the effects of maintaining biological activity, improving purity, and good step repeatability

Active Publication Date: 2018-04-20
SHENZHEN UNIV
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Problems solved by technology

However, due to the lack of existing separation and purification technology, it is still difficult to obtain condensed tannins with a degree of polymerization greater than 5, and the research and application of macromolecular plant polyphenols (the greater the degree of polymerization of tannins, the greater the molecular weight) has been limited.

Method used

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  • Extraction method for plant condensed tannin high polymers
  • Extraction method for plant condensed tannin high polymers

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Embodiment

[0045] see figure 1 , this embodiment takes the mangrove plant silique as the implementation object, and is used to extract the condensed tannin polymer in the mangrove plant.

[0046] S1 Select an appropriate amount of mature leaves of Mangroveaceae, put them into a freeze dryer, freeze-dry them at a temperature of -40 to -50°C for more than 72 hours, and then grind them evenly with a mortar;

[0047] S2 Take 100 g of the above-mentioned ground sample, add 500 mL of 70% acetone aqueous solution, conduct ultrasonic extraction for 30 min, and then centrifuge at a centrifugation rate of 19000 g. Then the supernatant was collected, and 70% acetone aqueous solution was added to the remaining precipitated part, followed by ultrasonic extraction and centrifugation. The above process was carried out three times in total.

[0048] The supernatants obtained from the above three centrifugation treatments were combined, and rotary evaporated at a temperature not higher than 30° C. to re...

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Abstract

The invention provides an extraction method for plant condensed tannin high polymers, which comprises the following steps: after being freeze-dried, a plant sample which is washed clean is ground andthen added with 70 percent of aqueous acetone solution; after ultrasonic extraction, centrifuging and rotary evaporation, extracted crude tannin extract is then extracted and degreased, and after organic solvent is removed, the remaining aqueous phase part is then freeze-dried; obtained crude tannin extract is dissolved into water and then loaded onto a first chromatographic column, distilled water, 60 percent methanol solution, 100 percent methanol solution and 70 percent acetone solution are sequentially adopted for washing, eluent with 70 percent acetone is collected and loaded onto a second chromatographic column, three types of mixtures of acetone, methanol and water which are composed according to different proportions are adopted for elution for three times, the three eluents are respectively collected, and thereby the condensed tannin high polymers with different polymerization degree peaks are obtained. The extraction method can effectively purify high-polymerization-degree condensed tannin in a plant, and provides a new approach for the field of the current research of structures and activities of macromolecular plant polyphenols.

Description

technical field [0001] The invention relates to a method for extracting and purifying plant tannins, in particular to a method for using plants to extract condensed tannin high polymers. Background technique [0002] Plant polyphenols (Plant polyphenols), also known as plant tannins (Vegetable tannins), have complex structures and various types. According to different chemical structures, they can be divided into condensed tannins, hydrolysable tannins, Phloroglucinol tannins (Phlorotannins) and polymer macromolecular polyphenols such as complex tannins (Complex tannins) containing condensed tannins and hydrolyzed tannins together, as well as flavonoids (Flavonoids), phenolic acids (Phenolic acids) ) and other small molecule polyphenols. [0003] Plant tannins have the characteristics of complexing with proteins (enzymes), polysaccharides, alkaloids and other biological macromolecules and heavy metal salts to form complexes, which is considered to be the main mechanism of a...

Claims

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Application Information

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IPC IPC(8): C08H7/00
CPCC08H6/00
Inventor 周海超韦萍萍雷安平胡章立李凤兰杨琼
Owner SHENZHEN UNIV
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