Reagent set for five-classification hematology analyzer and preparation method of reagent set

A blood cell analyzer and five-classification technology are applied in the field of preparation of reagent kits for five-class blood cell analyzers, which can solve the problems of import and the like, and achieve the effects of easy availability of raw materials, good promotion and use value, and reasonable assembly methods.

Inactive Publication Date: 2018-04-20
江苏荣盛嘉美生物试剂有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The technical problem to be solved by the present invention is to overcome the defect that the special reagents of the existing five-classification automatic blood cell analyzer need to be imported, and provide a reagent kit for the five-classification blood cell analyzer and its preparation method

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] A reagent set for a five-differentiation hematology analyzer, consisting of a diluent, a hemolytic agent and a staining solution, the diluent including: NaCl 5.5g / L, boric acid 1g / L, EDTA-K 2 0.2g / L, borax 0.4g / L and thiazolinone 0.3g / L;

[0016] Described hemolytic agent comprises hemolytic agent-WDF, hemolytic agent-WNR and hemolytic agent-SLS; Described hemolytic agent-WDF comprises NaCl 1.8g / L, EDTA-K 2 0.1g / L, polyoxyethylene ether surfactant 1.5g / L, quaternary ammonium salt 0.5g / L, succinic acid 0.5g / L; the hemolytic agent-WNR includes quaternary ammonium salt 1.0g / L, poly Oxyethylene ether surfactant 4.0g / L, succinic acid 0.4g / L; EDTA-Na 2 0.1g / L; the hemolytic agent-SLS includes: TritonX-100 1.0g / L, ionic surfactant 1.2g / L and ethylene glycol phenyl ether 3.0g / L;

[0017] The dyeing solution includes 0.012g / L of polymethine dye, 28g / L of methanol and 950g / L of ethylene glycol.

[0018] After the reagents are dosed, coarse filtration is performed first, and...

Embodiment 2

[0020] A reagent set for a five-differentiation hematology analyzer, consisting of a diluent, a hemolytic agent and a staining solution. The diluent includes: NaCl 7g / L, boric acid 1.5g / L, EDTA-K 2 0.4g / L, borax 0.6g / L and thiazolinone 0.5g / L;

[0021] Described hemolytic agent comprises hemolytic agent-WDF, hemolytic agent-WNR and hemolytic agent-SLS; Described hemolytic agent-WDF comprises NaCl 2.2g / L, EDTA-K 2 0.3g / L, polyoxyethylene ether surfactant 2g / L, quaternary ammonium salt 0.8g / L, succinic acid 0.7g / L; the hemolytic agent-WNR includes quaternary ammonium salt 1.5g / L, polyoxygen Vinyl ether surfactant 5.2g / L, succinic acid 0.6g / L; EDTA-Na 2 0.3g / L; the hemolytic agent-SLS includes: TritonX-100 1.5g / L, ionic surfactant 1.4g / L and ethylene glycol phenyl ether 3.7g / L;

[0022] The dyeing solution includes 0.03g / L of polymethine dye, 32g / L of methanol and 1000g / L of ethylene glycol.

[0023] After the reagents are dosed, coarse filtration is performed first, and th...

Embodiment 3

[0025] A reagent set for a five-differentiation hematology analyzer, consisting of a diluent, a hemolytic agent and a staining solution, the diluent including: NaCl 6g / L, boric acid 1.2g / L, EDTA-K 2 0.3g / L, borax 0.5g / L and thiazolinone 0.4g / L.

[0026] Further, the hemolytic agent-WDF includes NaCl 2g / L, EDTA-K 2 0.2g / L, polyoxyethylene ether surfactant 1.7g / L, quaternary ammonium salt 0.7g / L, succinic acid 0.55g / L; the hemolytic agent-WNR includes quaternary ammonium salt 1.2g / L, poly Oxyethylene ether surfactant 4.5g / L, succinic acid 0.5g / L; EDTA-Na 2 0.2g / L; the hemolytic agent-SLS is: TritonX-100 1.2g / L, ionic surfactant 1.5g / L and ethylene glycol phenyl ether 3.5g / L.

[0027] The dyeing solution comprises 0.025g / L of polymethine dye, 30g / L of methanol and 970g / L of ethylene glycol.

[0028] After the reagents are dosed, coarse filtration is performed first, and then ultrafiltration is performed with a 0.22 μm filter membrane before packaging.

[0029] The five-diff...

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PUM

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Abstract

The invention discloses a reagent set for a five-classification blood cell analyzer, which consists of a diluent, a hemolytic agent and a staining solution. The reagent set for the five-classification blood cell analyzer of the present invention has the advantages of reasonable formula, easy-to-obtain raw materials, easy processing, and low cost. Therefore, it has a good value for popularization and use; it changes the current situation that the domestic reagents for blood cell analyzers rely on imports for a long time, and The hemolytic agent-SLS in the reagent is a cyanide-free hemolytic agent, which greatly reduces the damage to the health of the production unit personnel and experiment operators, and also avoids the pollution of the environment caused by the use of cyanide. The reagent is suitable for the XN series five-differentiation automatic blood cell analyzer of Sysmex Corporation in Japan. The correlation coefficients between white blood cells, red blood cells, hemoglobin, platelets, etc. and imported XN reagents are all greater than 0.95.

Description

technical field [0001] The invention relates to a reagent, in particular to a reagent kit for a five-classification blood cell analyzer and a preparation method thereof. Background technique [0002] The five-category automatic blood cell analyzer is a blood routine testing instrument widely used in my country. However, at present, this type of instrument and matching reagents are imported. Bring additional financial burden, and cause inconvenience. Contents of the invention [0003] The technical problem to be solved by the present invention is to overcome the defect that the special reagents of the existing five-classification automatic blood cell analyzer need to be imported, and provide a reagent kit for the five-classification blood cell analyzer and a preparation method thereof. [0004] In order to solve the problems of the technologies described above, the present invention provides the following technical solutions: [0005] A reagent set for a five-differentiati...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N15/00
CPCG01N15/00G01N2015/0065
Inventor 赵军理高飞
Owner 江苏荣盛嘉美生物试剂有限公司
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