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Constructing method and application of ulcerative colitis animal model

An ulcerative colitis and animal model technology, applied in compound screening/testing, animal husbandry, etc., can solve problems such as poor individual resistance, death, and low dose, and achieve good individual resistance, wide animal sources, and stable models. Effect

Inactive Publication Date: 2018-04-24
DALI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, rats are commonly used in animal models of UC, and rabbits, guinea pigs, and mice are also occasionally used; and responses to disease are very different from humans and therefore cannot be directly generalized to humans
[0005] At present, the UC animal model established by dogs adopts adult German shepherd dogs or Beagles, which are easily affected by external environmental factors during breeding, and the large size of adult dogs makes it difficult to artificially induce UC. Procedures are difficult to standardize, making it difficult to effectively control for confounding factors associated with UC
And its individual resistance is poor, and it is easy to induce other diseases or even death during the modeling process
Furthermore, German shepherd dogs or beagle dogs did not avoid other diseases or deaths during the modeling process. The doses of chemical reagents for inducing UC disclosed in literature methods are relatively low, so the degree of inflammation induced is relatively different from human clinical manifestations. Large, unable to objectively and correctly reflect the disease process or evaluate the therapeutic effect of drugs

Method used

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  • Constructing method and application of ulcerative colitis animal model
  • Constructing method and application of ulcerative colitis animal model
  • Constructing method and application of ulcerative colitis animal model

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0082] 1. Test animals

[0083] Adult native dogs (Canis lupus familiaris), aged 1-2 years, weighing 6.0-12.0Kg, were provided by the Animal Hospital of Guizhou University. 2 weeks), healthy subjects were used for this test.

[0084] 2. Build the experimental model

[0085] 1. Grouping of test dogs and determination of basic physiological data

[0086] Twelve healthy adult dogs were randomly divided into 3 groups, 4 in each group, half male and half male. During the test period, all experimental animals were reared in single cages, provided with the same amount of food and drinking water every day, and the relevant data of all domestic dogs were measured the day before modeling.

[0087] 1.1 Observation of dogs' routine signs: Give dogs an appropriate amount of food every day, and record body weight, body temperature, respiratory rate (times / minute), and stool frequency, stool properties, and mental state within 12 hours (9:00 to 21:00).

[0088] 1.2 Blood routine testing ...

Embodiment 2

[0100] Scoring criteria for colonoscopy examination of acute UC model induced by acetic acid in dogs

[0101] In the active stage of UC, the mucosa of colonic lesions becomes diffuse hyperemia and edema, and the texture of blood vessels is blurred or disordered; as the disease progresses, the mucosa becomes rough, and the fine particles dispersed in it are brittle, with natural bleeding or contact bleeding; when the lesion is serious, There are multiple shallow ulcers, and severe ulcers become large and deep, and mucus and blood may ooze out of the intestinal lumen.

[0102] At present, there are many endoscopic scoring methods for judging UC at home and abroad, and different scoring methods have different emphasis on mucosal healing and disease activity. Among them, the Baron endoscopy scoring method mainly focuses on the degree of mucosal bleeding, and the Mayo colonoscopy scoring method focuses on the overall characteristics of the mucosa. The specific scoring levels are as...

Embodiment 3

[0111] Experimental Model Validation

[0112] 1. Grouping of test dogs and determination of basic physiological data

[0113] 16 qualified adult dogs were selected and randomly divided into 4 groups, 4 in each group, half male and half male. During the test period, all experimental animals were reared in single cages, provided with the same amount of food and drinking water every day, and the relevant data of all domestic dogs were measured the day before modeling.

[0114] 2. Establishment of a model of acetic acid-induced ulcerative colitis in domestic dogs

[0115] 10% MgSO one day before modeling 4 Gastrointestinal solution 5mL / kg, clean the intestinal tract, fasting for 24 hours, intramuscular injection of Sumianning at 0.15mL / kg for anesthesia, insert a polyethylene catheter from the anus to 20cm into the colon, and slowly inject 20mL of 7% acetic acid solution , keep the enema for 1min, head down and tail up, rinse with 50mL normal saline.

[0116] 3. Administration...

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Abstract

The invention relates to an experiment animal model, and discloses a constructing method and application of an ulcerative colitis (UC) animal model. According to the ulcerative colitis animal model, Canis lupus familiaris is used as a model animal, acetic acid is adopted to cause ulcerative colitis of Canis lupus familiaris, and basically, the experiment result can be directly applied and popularized to the human body. The modeling way is easy to operate, the model is stable, easy to repeat and low in cost, and the animal model selection with more druggability evaluations and curative effect evaluations is provided for development and research of drugs for treating UC.

Description

technical field [0001] The invention relates to the field of experimental animal models, in particular to a method for constructing an animal model of ulcerative colitis and its application. Background technique [0002] Ulcerative colitis (Ulcerative Colitis, UC) is a chronic non-specific ulcerative colitis characterized by ulceration and chronic inflammation, and it is a kind of inflammatory bowel disease (Inflammatory Bowl Disease, IBD). The etiology of UC is unknown, and the main clinical manifestations are diarrhea, abdominal pain, mucus, pus and blood in the stool and other gastrointestinal symptoms. The lesions mostly involve the rectum and sigmoid colon, and can also spread throughout the colon. The onset and remission periods of UC alternate repeatedly, the course of the disease is long, and the cure is difficult. The recurrence rate after cure is high, and there is a certain correlation with the incidence of colon cancer, and the prognosis is poor. Therefore, it is...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/02
CPCA01K67/02A01K67/027A01K2207/20A01K2207/25A01K2227/10A01K2267/03A61K49/0008
Inventor 赵昱徐在品刘衡张成桂余正勇巫秀美李成功曹学信
Owner DALI UNIV
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