A kind of composite microbial bacterial agent and its preparation method and application

A technology for compounding microbial inoculants and cultures, applied in the field of microbial inoculants and their preparation, can solve the problems of waste of resources, easy loss of bacterial cells, and difficulty in treating soap and detergents to achieve ideal requirements.

CN107964518BActive Publication Date: 2020-10-30江苏世邦生物工程科技有限公司
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Publication Date
2020-10-30

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Abstract

The invention discloses a compound microbial agent. The compound microbial agent is prepared from nitrophilous rhizobium ATCC 35116 culture, trichoderma longibrachiatum AS3.1029 culture and acremoniumATCC 48379 culture. By culture solution prepared by mixed culture of the nitrophilous rhizobium ATCC 35116 culture, the trichoderma longibrachiatum AS3.1029 culture and the acremonium ATCC 48379 culture, the compound microbial agent has an excellent treatment effect on sewage mineral fertilizers, various organics, colloids, macromolecular substances such as sugars and organic acids, and the like,and accordingly effectiveness in domestic sewage purification treatment is realized.
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Description

technical field

[0001] The invention relates to a microbial bacterial agent and its preparation method and application, in particular to a composite microbial bacterial agent for heavy metal wastewater treatment and its preparation method and application. Background technique

[0002] At present, the discharge of urban domestic sewage is the main pollution source of urban water in my country, and the treatment of urban domestic sewage is the top priority of urban water conservation and urban water environment protection at present and in the future. Domestic sewage is the sewage generated in daily life, which refers to a complex mixture of various forms of inorganic and organic matter, including: floating and suspended solid particles of large and small sizes, colloidal and gel-like diffusers, and pure solutions.

[0003] In the prior art, when using compound microbial bacterial agents to treat domestic sewage, bacterial agents and biological enzymes are often used to decomp...

Examples

Embodiment 1

[0024] Raw material components: 10 parts of azobium ATCC 35116 culture, 10 parts of Trichoderma longifolia AS3.1029 culture, 15 parts of Acremonium ATCC 48379 culture, 20 parts of nano silicon dioxide, 40 parts of activated carbon and plant ash 5 servings.

[0025] Preparation and application: (1) Expanded cultivation for the first time: Inoculate each strain in the raw material components into the first expanded medium respectively, the raw material components of the first expanded medium are: potato 200g / L, glucose 10g / L L, agar 15g / L and chloramphenicol 5g / L. The culture humidity is 25 parts, and the culture is expanded at 35°C for 3 days, and the cultures of each strain in the raw material components are respectively obtained;

[0026] (2) Expanded cultivation for the second time: the various bacterial cultures obtained in the expanded cultivation for the first time are uniformly mixed by raw material component weight parts, and the mixed bacterial culture suspension is p...

Embodiment 2

[0030] Raw material components: 5 parts of the culture of Azorhizobium ATCC 35116, 5 parts of the culture of Trichoderma longifolia AS3.1029, 10 parts of the culture of Acremonium ATCC 48379, 15 parts of the culture of Bifidobacterium dentatus ATCC 15423, 15 parts of Lactobacillus acidum ATCC43121 culture, 20 parts of nano silicon dioxide, 30 parts of activated carbon.

[0031] Preparation and application: (1) The first expanded culture: inoculate each bacterial strain in the raw material components to the first expanded medium, the first expanded medium raw material components are: potato 300g / L, glucose 30g / L , agar 20g / L and chloramphenicol 8g / L. The culture humidity is 35%, and the culture is expanded at 38°C for 4 days to obtain the cultures of various strains in the raw material components;

[0032] (2) Expanded cultivation for the second time: the various bacterial cultures obtained in the expanded cultivation for the first time are uniformly mixed by raw material comp...

Embodiment 3

[0036] Raw material components: 7 parts of culture of azorhizobium ATCC 35116, 8 parts of culture of Trichoderma longifolia AS3.1029, 12 parts of culture of Acremonium acremonium ATCC 48379. 10 parts of Bifidobacterium dentate ATCC 15423 culture, 8 parts of Lactobacillus acidophilus ATCC43121 culture, 15 parts of nano silicon dioxide, 35 parts of activated carbon and 7 parts of plant ash.

[0037] Preparation and application: (1) The first expanded culture: inoculate each bacterial strain in the raw material components to the first expanded medium, the first expanded medium raw material components are: potato 250g / L, glucose 20g / L , agar 18g / L and chloramphenicol 6g / L. The cultivation humidity is 30%, and the expansion cultivation is carried out at 37° C. for 4 days to obtain the cultures of various strains in the raw material components;

[0038] (2) Expanded cultivation for the second time: the various bacterial cultures obtained in the expanded cultivation for the first ti...