Method for induced differentiation of human placenta sub-totipotent stem cells into cardiac muscle cells and application of method

A technology of subtotipotent stem cells and cardiomyocytes, which is applied in the field of human placental subtotipotent stem cells to induce differentiation into cardiomyocytes, which can solve the problems of limited proliferation ability and difficult recovery

Inactive Publication Date: 2018-04-27
重庆斯德姆生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Current therapeutic approaches for heart failure focus on early angiogenesis and inhibiting further loss of cardiomyocytes, a terminally differentiated cell with extremely limited proliferative capacity and extremely difficult to recover from loss

Method used

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  • Method for induced differentiation of human placenta sub-totipotent stem cells into cardiac muscle cells and application of method
  • Method for induced differentiation of human placenta sub-totipotent stem cells into cardiac muscle cells and application of method
  • Method for induced differentiation of human placenta sub-totipotent stem cells into cardiac muscle cells and application of method

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Embodiment 1

[0021] A method for inducing the differentiation of human placental subtotipotent stem cells into cardiomyocytes proposed by the present invention comprises the following steps: inoculating human placental subtotipotent stem cells in a 24-well plate containing stem cell culture medium at 5000 cells / well, and waiting for the cells to adhere to the wall Finally, the stem cell culture medium was removed, and then the cardiomyocyte culture medium was added, cultured for 28 days, and the medium was changed every 3 days.

[0022] Stem cell culture medium includes:

[0023]

[0024] Cardiomyocyte culture medium includes:

[0025]

Embodiment 2

[0027] A method for inducing differentiation of human placental subtotipotent stem cells into cardiomyocytes proposed by the present invention comprises the following steps: inoculating human placental subtotipotent stem cells in a 24-well plate containing stem cell culture medium at 5000 cells / well, and waiting for the cells to adhere to the wall Finally, the stem cell culture medium was removed, and then the cardiomyocyte culture medium was added, cultured for 28 days, and the medium was changed every 3 days.

[0028] Stem cell culture medium includes:

[0029]

[0030] Cardiomyocyte culture medium includes:

[0031]

[0032]

Embodiment 3

[0034] A method for inducing differentiation of human placental subtotipotent stem cells into cardiomyocytes proposed by the present invention comprises the following steps: inoculating human placental subtotipotent stem cells in a 24-well plate containing stem cell culture medium at 5000 cells / well, and waiting for the cells to adhere to the wall Finally, the stem cell culture medium was removed, and then the cardiomyocyte culture medium was added, cultured for 28 days, and the medium was changed every 3 days.

[0035] Stem cell culture medium includes:

[0036]

[0037] Cardiomyocyte culture medium includes:

[0038]

[0039]

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Abstract

The invention discloses a method for induced differentiation of human placenta sub-totipotent stem cells into cardiac muscle cells. The method comprises the following steps: inoculating human placentasub-totipotent stem cells into a 24-pore plate which contains a stem cell nutrient solution; and after cell attachment, removing the stem cell nutrient solution, adding a myocardial cell nutrient solution, and culturing for 28 days, wherein the solution is changed every 3 days. The stem cell nutrient solution comprises a DMEM culture medium, hydroxyethyl starch, human serum albumin, potassium gluconate, sodium phenolsulfonate, asparaginic acid chelated calcium, taurine, placenta growth factors, fibroblast factors, glutamine, transferrins, polygahatous polysaccharides, astragali polysaccharideand hyaluronic acid. The myocardial cell nutrient solution comprises an RPMI-1640 culture medium, trehalose, taurine, fibroblast growth factors, epidermal growth factors, potassium gluconate, asparaginic acid chelated selenium, phenylalanine, progesterone, tea polyphenols, niacinamide, ginseng root powder and powder of vines of multiflower knotweed.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to a method for inducing differentiation of human placental subtotipotent stem cells into cardiomyocytes and an application thereof. Background technique [0002] Heart failure caused by cardiomyocyte dysfunction is a major disease worldwide, and the final cause of death in heart failure patients is mainly loss of cardiac pumping function or arrhythmia. The one-year mortality rate of patients with severe heart failure exceeds 50%. Although heart transplantation is possible for terminally ill patients, about 20% of patients die while waiting for organ transplantation due to lack of donor organs. Due to the high morbidity and mortality of heart failure, the death of transplanted hearts, complications such as immune rejection, and the late failure of transplanted hearts, it is urgent to develop new therapeutic methods to improve the function of cardiomyocytes and prevent the occur...

Claims

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Application Information

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IPC IPC(8): C12N5/077A61K35/34A61P9/00
CPCC12N5/0657A61K35/34C12N2500/12C12N2500/24C12N2500/32C12N2500/38C12N2500/76C12N2501/10C12N2501/11C12N2501/115C12N2501/392C12N2501/90C12N2501/905C12N2501/999C12N2506/025
Inventor 赵翔熊雪孙静秦连荣
Owner 重庆斯德姆生物技术有限公司
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