Method for induced differentiation of human placenta sub-totipotent stem cells into cardiac muscle cells and application of method
A technology of subtotipotent stem cells and cardiomyocytes, which is applied in the field of human placental subtotipotent stem cells to induce differentiation into cardiomyocytes, which can solve the problems of limited proliferation ability and difficult recovery
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Embodiment 1
[0021] A method for inducing the differentiation of human placental subtotipotent stem cells into cardiomyocytes proposed by the present invention comprises the following steps: inoculating human placental subtotipotent stem cells in a 24-well plate containing stem cell culture medium at 5000 cells / well, and waiting for the cells to adhere to the wall Finally, the stem cell culture medium was removed, and then the cardiomyocyte culture medium was added, cultured for 28 days, and the medium was changed every 3 days.
[0022] Stem cell culture medium includes:
[0023]
[0024] Cardiomyocyte culture medium includes:
[0025]
Embodiment 2
[0027] A method for inducing differentiation of human placental subtotipotent stem cells into cardiomyocytes proposed by the present invention comprises the following steps: inoculating human placental subtotipotent stem cells in a 24-well plate containing stem cell culture medium at 5000 cells / well, and waiting for the cells to adhere to the wall Finally, the stem cell culture medium was removed, and then the cardiomyocyte culture medium was added, cultured for 28 days, and the medium was changed every 3 days.
[0028] Stem cell culture medium includes:
[0029]
[0030] Cardiomyocyte culture medium includes:
[0031]
[0032]
Embodiment 3
[0034] A method for inducing differentiation of human placental subtotipotent stem cells into cardiomyocytes proposed by the present invention comprises the following steps: inoculating human placental subtotipotent stem cells in a 24-well plate containing stem cell culture medium at 5000 cells / well, and waiting for the cells to adhere to the wall Finally, the stem cell culture medium was removed, and then the cardiomyocyte culture medium was added, cultured for 28 days, and the medium was changed every 3 days.
[0035] Stem cell culture medium includes:
[0036]
[0037] Cardiomyocyte culture medium includes:
[0038]
[0039]
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Abstract
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Application Information
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