Hyper-spectral light sheet light field microscopic imaging system and method based on camera array
A camera array and light field microscopy technology, applied in microscopes, optics, optical components, etc., can solve the problem of low image resolution and achieve the effects of high spatial resolution, great flexibility and scalability of the system
Inactive Publication Date: 2018-04-27
TSINGHUA UNIV
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This method requires a compromise between spatial and angular resolution, and the resulting image resolution is much lower than that of the camera
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[0028] Embodiments of the present invention are described in detail below, examples of which are shown in the drawings, wherein the same or similar reference numerals designate the same or similar elements or elements having the same or similar functions throughout. The embodiments described below by referring to the figures are exemplary and are intended to explain the present invention and should not be construed as limiting the present invention.
[0029] The hyperspectral light sheet light field microscopic imaging system and method based on the camera array proposed according to the embodiments of the present invention will be described below with reference to the accompanying drawings. First, the hyperspectral light sheet based on the camera array proposed according to the embodiments of the present invention will be described with reference to the accompanying drawings Light field microscopy imaging system.
[0030] figure 1 It is a schematic structural diagram of a ca...
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The invention discloses a hyper-spectral light sheet light field microscopic imaging system and method based on a camera array. The system includes a lighting module which is used for producing a light sheet and making the light sheet carry out scanning on the z axis, a microscope which is used for magnifying a microscopic sample and imaging the microscopic sample to an image plane through a camera lead-out port, a magnifying module of an imaging plane, an imaging lens array which is used for acquiring sample images of the microscopic sample at different angles of view, a filter-containing sensor array of which the sensors are arranged in one-to-one correspondence with the imaging lenses of the imaging lens array to record sample image spatial information obtained by the corresponding imaging lenses and sample spectral information obtained by the corresponding filters, and a controller which is used for synchronizing and calibrating the collection of the sample images to obtain light field video and hyper-spectral information and reconstructing the 3D image and spectral components of the sample to realize microscopic imaging. The system can realize high-performance microscopic imaging, and has high spatial resolution and greater flexibility and expansibility.
Description
technical field [0001] The invention relates to the technical field of microscopic imaging, in particular to a hyperspectral light sheet light field microscopic imaging system and method based on a camera array. Background technique [0002] In related technologies, Light Sheet Microscopy (Light Sheet Microscopy) uses mutually perpendicular illumination light paths and imaging light paths to improve imaging quality. The illumination optical path generates a thin layer of light beam (light sheet) to illuminate the sample from the side, and the imaging optical path directly performs surface imaging on the sample, thereby avoiding the interference of other layers and improving the imaging resolution. Light sheet microscopy adopts surface imaging, and its imaging speed is much higher than that of confocal microscopy; at the same time, since only the sample at the light sheet is illuminated, the influence of phototoxicity and photobleaching on the sample is greatly reduced. By c...
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IPC IPC(8): G02B21/36
CPCG02B21/361G02B21/365
Inventor 谢浩韩晓霏熊博戴琼海
Owner TSINGHUA UNIV