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Photo-thermal heterodyning microimaging detection system and method

A technology of microscopic imaging and detection system, which is applied in measurement devices, material analysis by optical means, instruments, etc., can solve the problems of bleaching, unstable fluorescent labeling signals, and inability to directly image non-fluorescent objects, and achieves improved signal-to-noise. ratio, improve extraction and resolution capabilities, and overcome the effect of direct imaging

Inactive Publication Date: 2019-01-18
HARBIN INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] The purpose of the present invention is to overcome the problem that the current fluorescence microscopic imaging technology / system cannot directly image non-fluorescent objects, and the signal instability phenomenon exists in the use of fluorescent markers, and provide a photothermal heterodyne microscopic imaging detection system and method, The photothermal heterodyne microscopic imaging is a new type of microscopic imaging technology, which mainly utilizes the photothermal effect and characteristics of the measured object, is less affected by background scattering, has the advantages of stable signal and good imaging effect, especially This technology can solve the problems of photobleaching and blinking of fluorescent labels

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specific Embodiment approach 1

[0024] Specific implementation mode one: as figure 1 As shown, what is described in this embodiment is a photothermal heterodyne microscopic imaging detection system. Chromatic mirror 6, 1 / 4 wave plate 7, cage frame support 8, polarization beam splitter cube 9, 600nm long-pass filter 10, beam splitter 11, avalanche photodiode module 12, CCD camera 13, piezoelectric movement Table control line 14, SMA to BNC line 15, Ethernet line 16, lock-in amplifier SR84417, two-dimensional piezoelectric mobile station controller 18, SMA to BNC line 19, GPIB data line 20, USB control line 21, computer 22, office Table 23, USB data cable 24, 638nm laser 25, 532nm laser 26, acousto-optic modulator driver 27, SMA control line 28, frame support rod 29, 20×beam reducer 30, rotating mobile table 31, acousto-optic modulator 32. Lifting mobile platform 33, mirror one 34, optical platform 35, cage frame connecting rod 36, mirror two 37, three-dimensional mobile platform 38, objective lens cantilever...

specific Embodiment approach 2

[0027] Specific implementation mode two: as figure 1 , figure 2 As shown, a photothermal heterodyne microscopic imaging detection method using the photothermal heterodyne microscopic imaging detection system of the first embodiment, the specific steps of the method are as follows:

[0028] Step 1: Determine the test sample 3 to be measured, clamp and fix it on the two-dimensional piezoelectric mobile platform 2, and the two-dimensional piezoelectric mobile platform 2 controls the horizontal movement of the test sample 3;

[0029] Step 2: first turn on the computer 22, the lock-in amplifier 17, the two-dimensional piezoelectric mobile controller 18, the CCD camera 13, the 638nm laser 25, the 532nm laser 26, and the acousto-optic modulator driver 27;

[0030] Step 3: Control the 638 laser 25 to output excitation light through the computer 22, turn on the 532nm laser 26 at the same time, and then adjust the optical path, so that the two laser beams are combined through the opti...

specific Embodiment approach 3

[0035] Specific implementation mode three: as figure 2 As shown, the photothermal heterodyne microscopic imaging detection method described in the second specific embodiment, the optical path propagation sequence in the method is: the excitation light emitted by the 532nm laser 26 passes through the 20× beam reducer 30 to reduce the beam diameter, Entering the acousto-optic modulator 32, through the acousto-optic modulation, the output light becomes modulated light with high-frequency modulation of light intensity; then passes through the dichroic mirror 6 to combine with the probe light, and is focused by the 100×objective lens 40 to act on the detection sample 3 above; the probe light emitted by the 638nm laser 25 first passes through the polarization beam splitter cube 9 to output linearly polarized light, then passes through the 1 / 4 wave plate 7 to realize the circular polarization of the probe light, and combines with the excitation light at the dichroic mirror 6 Work to...

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Abstract

The invention provides a photo-thermal heterodyning microimaging detection system and method, and belongs to the technical field of microimaging detection. The system comprises a right angle bracket,a two-dimensional piezoelectric moving table, a supporting bar, a supporting bar holder, a dichroscope, a 1 / 4 wave plate, a cage type mirror bracket supporting seat, a polarization beam splitter cube,a 600nm long pass filter, a spectroscope, an avalanche photodiode module, a CCD camera, a lock-in amplifier, a two-dimensional piezoelectric moving table controller, a computer, an office table, a 638nm laser, a 532nm laser, an acoustic optical modulator driver, a mirror bracket supporting rod, a 20* beam reduction mirror, a rotation moving table, an acoustic optical modulator, a lifting moving table, a first reflector, an optical platform, a cage type mirror bracket connecting rod, a second reflector, a three-dimensional moving table, an objective lens cantilever mounting rack, a 100* objective lens, a dark box and various connecting lines. The photo-thermal heterodyning microimaging detection system provided by the invention has the advantages of greatly improving the signal extractionand resolving power by utilizing a heterodyning detection manner, thereby improving the signal-to-noise ratio of the photo-thermal microimaging.

Description

technical field [0001] The invention belongs to the technical field of microscopic imaging detection, and specifically relates to a photothermal heterodyne microscopic imaging detection system and method, which are suitable for photothermal imaging and evaluation of nanoscale materials such as metal nanoparticles, carbon nanotubes, and biological tissue cells. Background technique [0002] With the continuous development of science and technology, the imaging observation and characteristic analysis of nanoscale objects has always been a hot research direction. Far-field optical microscopic imaging technology is the earliest microscopic imaging technology proposed, which has the advantages of non-destructive, good specificity and deep penetration. Nano scale. With the development of microscopic detection technology, fluorescence microscopic imaging (microscope) technology has gradually developed. It has the advantages of high signal-to-noise ratio, strong specificity and mul...

Claims

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Application Information

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IPC IPC(8): G01N21/01G01N21/63
Inventor 刘俊岩王永辉王飞王扬
Owner HARBIN INST OF TECH
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