A Microscopic Imaging Method Based on Phase Encoded Single Lens

Abstract

The invention discloses a microscopic imaging method based on a phase-encoded single lens. Only one single lens with a phase-encoded function is used for imaging, and combined with a follow-up processing algorithm, the microscopic imaging resolution equivalent to that of an existing precision commercial microscope can be realized. , suitable for portable detection of biological tissues and cells under field conditions. First, the imaging system uses a phase-encoded single lens with two aspheric surfaces to image biological samples. The image sensor is located at the conjugate position of the biological sample under G light illumination for image acquisition; then the algorithm is used to image the collected Image processing: respectively execute the auto-focus algorithm on the R\B two channels of the collected images, and reconstruct and recover the quasi-focus images of the R\B two channels; for the quasi-focus images of the R\G\B three channels The kernel function of the trained GAN is used for high-resolution image reconstruction; finally, the reconstructed R\G\B images are subjected to image matching and color fusion.

Description

technical field [0001] The invention belongs to the field of microscopic imaging, in particular to a microscopic imaging method based on a phase-encoding single lens. Background technique [0002] At present, one of the common detection methods for observing the microscopic world is to use microscopes to visually detect the collected biochemical samples, such as phase contrast microscope, differential interference contrast microscope, fluorescence microscope, laser confocal microscope, which has promoted the level of life science research. With higher resolution and imaging quality, it provides a strong imaging basis for disease diagnosis, especially for the early diagnosis of major malignant diseases, but they are usually expensive, bulky and cumbersome, which are not conducive to the field. use. [0003] With the development of the times, the demand for portable biochemical detection and data analysis equipment for field biochemical detection in the field of mobile medica...

AI Technical Summary

Problems solved by technology

Among them, the free-form surface method is difficult to eliminate all aberrations because the lens cannot satisfy the optical path difference for all rays of light in a large field of view, and the surface processing is difficult, and the processing quality cannot be guaranteed.

Gradient-index lens method, although capable of eliminating spherical aberration, does not work well in large spatial bandwidth product imaging systems

The metalense method is usually made by electron beam lithography and focused ion beam etching to produce non-periodic nanoscale structures on the lens surface, such as Capasso of Harvard University in "Metalenses at visible wavelengths: Diffraction-limited focusing and subwavelength resolution imaging" In this paper, it is proposed to use a metalens composed of micro-nano structures to realize imaging in the visible light band. However, at present, the diameter of the metalens that can realize single-lens imaging is less than mm, the field of view is small, and the processing is very complicated, which is not yet possible. Applied to common imaging equipment

Images