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A kind of extraction method of flavonoids from Daphne genkwa

An extraction method, a technology of flower flavonoids, which is applied in the field of extraction of flavonoids, and can solve the problems of not separating effective substances, etc.

Active Publication Date: 2021-04-20
ZHEJIANG OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Therefore how to effectively purify flavonoids from Shuigenghua is very urgent. For example, the inventor once published a paper on Heilongjiang Medical Science Journal "Studies on Antioxidant Activity and Antibacterial Activity of Shuigenghua Extract in Vitro", It only extracted petroleum ether extract, chloroform extract, ethyl acetate extract and water extract by organic solvent extraction, and analyzed its antioxidant activity and antibacterial activity in vitro, but did not No effective substance was isolated

Method used

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  • A kind of extraction method of flavonoids from Daphne genkwa
  • A kind of extraction method of flavonoids from Daphne genkwa

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] A method for extracting genkwa flavonoids, the purification method comprising the following steps:

[0022] (a) Take 10 parts of Daphne genkwa stems and put them in a pulverizer to crush them, pass through a 100-mesh sieve, add 20 parts of distilled water and 0.5 parts of cellulase, adjust the pH to 5.5, raise the temperature to 48°C for 3 hours after enzymatic hydrolysis , adding 200 parts of ethyl acetate, 200W ultrasonic treatment for 30 minutes, centrifugal filtration to take the filtrate, standing and layering to take the organic layer, and obtaining the crude extract of genkwa flavonoids;

[0023] (b) Concentrate the crude extract of genkwa flavonoids in step (a) to one-fifth of the original volume, then add 5 parts of activated carbon, stir for 0.5 hours, let it stand for adsorption for 1 hour, filter to obtain the filtrate, and remove it under reduced pressure Ethyl acetate, obtains the crude product of genkwa genkwa flavonoids;

[0024] (c) Take 10 parts of th...

Embodiment 2

[0027] A method for extracting genkwa flavonoids, the purification method comprising the following steps:

[0028] (a) Take 10 parts of Daphne genkwa stems and put them in a pulverizer to crush them, pass through a 300-mesh sieve, add 20 parts of distilled water and 2 parts of cellulase, adjust the pH to 6.5, raise the temperature to 60°C for 5 hours after enzymatic hydrolysis , adding 200 parts of petroleum ether, 200W ultrasonic treatment for 30 minutes, centrifugal filtration to take the filtrate, standing and layering to take the organic layer, and obtaining the crude extract of genkwa flavonoids;

[0029] (b) Concentrate the crude extract of genkwa flavonoids in step (a) to one-fifth of the original volume, then add 10 parts of activated carbon, stir for 0.5 hours, let it stand for adsorption for 3 hours, filter to obtain the filtrate, and remove it under reduced pressure Petroleum ether, obtains the crude product of genkwa flavonoids;

[0030] (c) Take 10 parts of the c...

Embodiment 3

[0033] A method for extracting genkwa flavonoids, the purification method comprising the following steps:

[0034] (a) Take 10 parts of Daphne genkwa stems and put them in a pulverizer to crush them, pass through a 200-mesh sieve, add 20 parts of distilled water, 1.5 parts of cellulase, adjust the pH to 5.8, raise the temperature to 55°C for 4 hours after enzymatic hydrolysis , adding 200 parts of dichloromethane, 200W ultrasonic treatment for 30 minutes, centrifugal filtration to take the filtrate, standing and layering to take the organic layer, and obtaining the crude extract of genkwa flavonoids;

[0035] (b) Concentrate the crude extract of genkwa flavonoids in step (a) to one-fifth of the original volume, then add 8 parts of activated carbon, stir for 0.5 hours, let it stand for adsorption for 2 hours, filter to obtain the filtrate, and remove it under reduced pressure Dichloromethane obtains the crude product of genkwa flavonoids;

[0036](c) Take 10 parts of the crude...

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Abstract

The invention discloses a method for extracting flavonoids from genkwa. Firstly, breaking genkwa. and using cellulase in an aqueous solution to decompose the cellulose in genkwa. The flavonoids were extracted, and then purified by recrystallization and column chromatography to obtain pure flavonoids. The invention has the following beneficial effects: (1) mild extraction conditions and simple operation; (2) high extraction efficiency; (3) simple purification process and low purification cost.

Description

technical field [0001] The invention relates to a method for extracting flavonoids, in particular to a method for extracting flavonoids from genkwa genkwa. Background technique [0002] Pemphisacidula (scientific name: Pemphisacidula) is a small shrub of the genus Pemphisacidula in the family Pemphisaceae. Its distribution range is narrow and remote. It is mainly distributed on the southern coast of Taiwan Island and other tropical coasts in the Eastern Hemisphere. It is currently endangered. Water genkwa is often used to make valuable bonsai. At present, the research mainly focuses on its growth environment and distribution, taxonomy, reproduction and genetic diversity, etc., but there are few studies on its plant components and pharmacological activities. In only a few research results, it is shown that only through simple organic solvent extraction, the organic solvent extract is obtained, but the active ingredients inside it are not extracted. [0003] Flavonoids are wi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/185A61P39/06
CPCA61K36/185A61K2236/15A61K2236/19A61K2236/33A61K2236/35A61K2236/51A61K2236/53A61K2236/55
Inventor 闻正顺姚利阳曲有乐
Owner ZHEJIANG OCEAN UNIV