Lytic vibrio phage ValSw4-1 as well as bactericidal composition containing phage and application thereof
A valdsh-1 and bacteriophage technology, applied in the biological field, can solve the problems of short duration of lysis ability, narrow lysis host spectrum, and easy resistance of host bacteria, and achieve good control effect, low production cost and high titer. Effect
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[0093] The present invention collects environmentally friendly fish and shrimp samples from the water samples of the Shanwei shrimp farming area, and isolates 7 pathogenic Vibrio strains from the lesions. The phage screening flow chart of the present invention is as follows figure 1 Shown. The pathogenicity of the isolated Vibrio was confirmed through field experiments, and it was determined that 5 strains were extremely pathogenic. LD 50 4 CFU / mL, action time <100h.
[0094] The host spectrum of the Vibrio phage in 32 strains of different species and genus was determined by the plate spot method, according to the presence or absence of transparent spots (Table 1), and it was concluded that the phage had strong host specificity and was a Vibrio It is a specific cleavage phage.
[0095] Table 1 Host spectrum
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[0097]
[0098] Remarks: "+" means that it can be lysed, "-" means that it cannot be lysed; the above strains are all isolated from aquaculture fish an...
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