PCR (Polymerase Chain Reaction) system for detecting porcine viral reproductive disorder diseases and detection method

Abstract

The invention discloses a PCR (Polymerase Chain Reaction) system for detecting porcine viral reproductive disorder diseases and a detection method. The detection method comprises the following steps:extracting DNA and RNA of viruses, performing reverse transcription on the RNA, and simultaneously, performing specific primer design of CSFV (classical swine fever virus), PPV (porcine parvovirus), PRRSV (porcine reproductive and respiratory syndrome virus) and JEV (Japanese encephalitis virus); then, optimizing PCR reaction conditions of the designed primers so as to determine an optimal multi-PCR mode; and performing a test according to the optimal multi-PCR reaction conditions, verifying the specificity, sensibility and stability of multi-PCR, simultaneously, performing clinical sample detection, and verifying the operation feasibility of the method. Furthermore, the invention also discloses a primer sequence for simultaneously detecting the CSFV, the PPV, the PRRSV and the JEV. The multi-PCR system and the detection method of the four porcine viral reproductive disorder diseases have the advantages that high specificity and sensibility of the conventional PCR are kept, operation steps are reduced, the purpose of simultaneously detecting multiple microorganisms by amplifying once is realized, the time is greatly saved, and the expenditures are saved.

Description

technical field [0001] The invention relates to the fields of agriculture and animal quarantine, in particular to a PCR system and a detection method for detecting porcine viral reproductive disorders. Background technique [0002] With the continuous expansion of large-scale farms in my country, intensive feeding methods have greatly increased the probability of contact between pigs. The increase in the probability of contact has accelerated the spread of the disease, and at the same time, the sick pigs often show mixed infections. Among them Porcine reproductive disorder has become one of the most important diseases in large and medium-sized pig farms, and it is distributed globally. Currently, swine fever (CSF), porcine reproductive and respiratory syndrome (PRRS), porcine circovirus disease (PCVDA), porcine parvovirus disease (PPI), and porcine Japanese encephalitis (JE) are the most common and serious reproductive disorders, causing huge economic losses to the pig industr...

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Problems solved by technology

For gene chips, the traditional chip platform relies on fluorescent labeling technology, requires expensive scanning equipment for scanning, and the operation process is complicated, which requires high technical requirements and is difficult to promote at the grassroots level.

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