Fermentation medium for producing doramectin, and preparation method and application thereof

A fermentation medium and doramectin technology, applied in the field of fermentation engineering, can solve the problems of reduced extraction yield, unstable quality, high viscosity of fermentation liquid, etc., to improve the fermentation level, improve the metabolism and utilization, and avoid the paste of the material liquid Effect

Active Publication Date: 2018-05-11
PEKING UNIV FOUNDER GRP CO LTD +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Problems with nitrogen sources, including the adverse effects of unstable animal nitrogen sources on the quality and fermentation effect of the ferment

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] This example is used to illustrate the formulation and preparation method of the fermentation medium of the present invention.

[0047] 1. Formula:

[0048] Cottonseed cake powder 40g / L, corn gluten powder 20g / L, maltose 200g / L, potassium dihydrogen phosphate 0.5g / L, sodium chloride 3 / L, magnesium sulfate 2.5g / L, calcium carbonate 1g / L, foam enemy 1g / L. Drinking water to volume.

[0049] 5% NaOH solution to adjust the pH to 6.8-7.2. Sterilize at 119-123°C for 30 minutes.

[0050] 2. Preparation method:

[0051](1) According to the above formula, feed cottonseed cake powder and corn gluten powder with a particle size of 10 mesh to 40 mesh into the fermenter, add drinking water to 70% of the constant volume, and stir with air, and control the ventilation flow to ensure that the feed liquid Just roll over completely.

[0052] (2) Preheat the fermenter, and monitor the temperature of the feed liquid in the fermenter, make the feed liquid reach 40-60°C, and keep it for ...

Embodiment 2

[0055] The difference between this embodiment and Example 1 is that the formula is: cottonseed cake powder 50g / L, corn gluten powder 15g / L, sucrose 100g / L, potassium dihydrogen phosphate 5g / L, sodium chloride 0.2 / L, magnesium sulfate 1g / L, calcium carbonate 0.5g / L, foam enemy 1g / L. Drinking water to volume.

experiment example

[0068] Inoculate the seed culture liquid that has completed the seed culture stage into the fermentation medium obtained in Example 1 and Comparative Examples 1-5, respectively, for fermentation.

[0069] The fermentation broth was observed in the early, middle and late stages, and the fermentation broth of 120h and 360h were sampled for detection.

[0070] turn out:

[0071] In Example 1, there is no paste phenomenon in the feed liquid in the whole fermentation process, and the growth in the early stage is slightly slower than that in Comparative Example 1, but the growth in the middle and late stages is very good, with a potency of 700 mg / L at 120 hours, and a potency of 2900 mg / L after 360 hours of fermentation.

[0072] Comparative Example 1 Before 120 hours of fermentation, the growth of the unit was normal, and the potency was 800 mg / L at 120 hours; but from the beginning of 120 hours of fermentation, the phenomenon of paste in the feed liquid was obvious, and the growth...

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Abstract

The invention relates to the field of fermentation engineering, and concretely discloses a fermentation medium for producing doramectin, and a preparation method and an application thereof. The fermentation medium adopts cottonseed cake powder preheated at 40-60 DEG C and having a granularity of 10-40 meshes and corn protein powder as a combined nitrogen source, and is better than fermentation media using single nitrogen sources in Streptonmyces avermitilis metabolism, so the production of doramectin is benefited. The control of granularity of the cottonseed cake powder and the corn protein powder is combined with the preheating treatment, so the paste formation phenomenon of a feed liquid in fermentation process (especially the middle and late fermentation stage) is effectively avoided, and the fermentation level of doramectin is significantly improved.

Description

technical field [0001] The invention relates to the field of fermentation engineering, in particular to a fermentation medium for producing doramectin. Background technique [0002] Doramectin is a new type of macrolide antiparasitic drug, which is biosynthesized by mutant Streptomyces avermitilis in a fermentation mode. In comparison, its anti-parasitic range is wider, its insecticidal activity is higher, and its effective time for preventing parasitic re-infection is longer. It has high development potential as a new veterinary anti-parasitic drug. [0003] At present, there are many problems in the process of fermenting and producing doramectin, which leads to low fermentation yield and high cost of doramectin. Among them, the influence of nitrogen source on fermentation yield was obvious. In the production cost of doramectin, the cost of nitrogen source accounts for about 30% of the fermentation cost. [0004] Problems with nitrogen sources include the adverse effects...

Claims

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Application Information

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IPC IPC(8): C12P19/62C12R1/465
CPCC12P19/62
Inventor 吴峰张葵周镪
Owner PEKING UNIV FOUNDER GRP CO LTD
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