Method and reagent for screening PCR-SSP of Vel-negative blood group gene

Abstract

The invention discloses a method and reagent for screening PCR-SSP of a Vel-negative blood group gene. The method comprises respectively designing specific amplification primers (indicated by +p and -p) according to a Vel blood group gene SMIM1 wild-type sequence and a SMIM1 c. 64-80 deletion mutant sequence, determining the optimal number of gathered specimens, carrying out gathering according toa certain quality (n) and amplifying the gathered specimens through the specific primer -p of the deletion mutant sequence. The deletion mutant sequence is a rare sequence so that the number of the reactively gathered target bands is small and the process can reduce the screening time and economic cost by n times. Aiming at the reactive gathering, single gathered specimen is respectively amplified through the primers +p and -p, the genotype of the single specimen participating in the gathering is initially determined and the genotype of the single specimen is verified by gene sequencing. Themethod and reagent can specifically and accurately identify the Vel blood group gene, can greatly reduce the time and economic cost of the screening work and can improve screening efficiency.

Description

technical field [0001] The invention relates to a genotyping detection method, in particular to a PCR-SSP method and reagent for screening Vel-negative blood group genes. Background technique [0002] The Vel blood group is the 34th blood group system newly named in 2013. This system has only one antigen Vel. Vel is expressed on the surface of most red blood cells. Vel negative (Vel-) is a rare phenotype. Anti-Vel can cause severe intravascular hemolytic reactions and hemolytic disease of the newborn. Studies have found that the antigen Vel is encoded and expressed by SMIM1 located on chromosome 1. This gene contains 4 exons, of which the coding region is located in the 3rd and 4th exons. When the 3rd exon occurs c.64_80 consecutive 17 nuclei When the homozygous deletion mutation of nucleotides, the reading frame of protein translation changes, the translation process is terminated prematurely, and the blood group protein SMIM1 is not expressed, resulting in Vel-rare phenot...

AI Technical Summary

Problems solved by technology

There are very few studies on Vel blood type in my country. With reference to the ratio of Vel- in the world, large-scale screening samples may be required to find Vel-rare individuals, which means huge time and economic costs

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