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D-dimer immunofluorescence quantitative kit and preparing method thereof

An immunofluorescence and dimer technology, which is used in the field of immunological detection to achieve the effect of convenient operation, high precision and good peak output effect.

Inactive Publication Date: 2018-05-11
ZHONGSHAN CHUANGYI BIOCHEM ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are few studies on how to effectively improve product performance by improving the carrier part including sample pads and bonding pads.

Method used

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  • D-dimer immunofluorescence quantitative kit and preparing method thereof
  • D-dimer immunofluorescence quantitative kit and preparing method thereof
  • D-dimer immunofluorescence quantitative kit and preparing method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] A kind of preferred formula of storage solution in the application of the present invention: the mass concentration of PB is 20mM, the mass concentration of BSA is 1.8%, the mass concentration of Tween-80 is 0.5%, the mass concentration of glucose is 0.5%, the mass concentration of glycine is 2%, the mass concentration of PEG4000 is 1%, the mass concentration of PEG20000 is 1.5%, the mass concentration of Proclin300 is 0.03%, and the pH is 7.8-8.0.

[0050] The preparation method of the storage solution is as follows: weigh 0.25 g of glucose and dissolve it with 45 mL of pure water. Add 1mL of 100mM PB stock solution prepared in advance, shake and mix well. Add 1g glycine, 0.5g PEG4000, 0.75g PEG20000 and 0.9gBSA successively, shake and mix well; add 0.25mL Tween-80 with a sample gun, mix well repeatedly; add 15μL Proclin300, shake and mix well, adjust pH with 1M HCl Value to 7.8 ~ 8.0. Finally, the volume was adjusted to 50 mL at room temperature and sterilized by fi...

Embodiment 2

[0053] A preferred formulation of the bonding pad pretreatment solution in the application of the present invention: the mass concentration of PVA is 2.0%, the mass concentration of TritonX-100 is 0.8%, the mass concentration of sucrose is 1.25%, the mass concentration of Proclin300 or sodium azide is 0.03%, pH7.2~7.4.

[0054] The preparation method of the bonding pad pretreatment liquid is as follows: Weigh 10 g of PVA, add 480 ml of pure water to soak overnight, place at 60° C. and heat to dissolve. Add 4 mL of Triton X-100 with a sample gun, and beat repeatedly; weigh 6.25 g of sucrose, put it into a magnetic stirrer to dissolve; add 150 μL of Proclin300, adjust the pH value to 7.2-7.4 with 1M HCl, and oscillate to mix. Finally, the volume was adjusted to 500 mL at room temperature, and the filter was sterilized to prepare the binding pad pretreatment solution.

Embodiment 3

[0056] The specific preparation method of the present embodiment provides a kind of D-dimer immunofluorescence quantitative test strip is as follows:

[0057] (1) Preparation of storage solution: the mass concentration of PB is 20mM, the mass concentration of BSA is 1.8%, the mass concentration of Tween-80 is 0.5%, the mass concentration of glucose is 0.5%, the mass concentration of glycine is 2%, PEG4000 The mass concentration of PEG20000 is 1%, the mass concentration of PEG20000 is 1.5%, the mass concentration of Proclin300 is 0.03%, and the pH is 7.8~8.0, according to the above formula preparation and mixing, filter and sterilize to obtain the storage solution;

[0058] (2) Preparation of the sample pad: Soak the glass cellulose membrane with the sample pad treatment solution for 10 minutes, place it in a drying room at 37°C and 30% humidity, and dry it for 5 hours to prepare the sample pad for use;

[0059] (3) Preparation of the bonding pad: Soak the glass cellulose membr...

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Abstract

The invention discloses a D-dimer immunofluorescence quantitative kit and a preparing method thereof. A sample pad, a combination pad, a nitrocellulose film and a water absorption pad are sequentiallyattached to a bottom lining of the kit in a lap joint mode. The combination pad is soaked in combination pad preprocessing liquid and dried. A D-dimer monoclonal antibody-fluorescent microsphere coupler is sprayed on the combination pad, and the D-dimer monoclonal antibody-fluorescent microsphere coupler is stored and diluted by storage liquid. Due to the improvement, the content of D-dimer in ahuman serum sample is accurately detected. Meanwhile, the kit is good in peak appearance effect, high in precision and high in sensitivity in the detecting process, and is quite excellent in stability, low in cost and convenient to operate.

Description

technical field [0001] The invention relates to the field of immunological detection, in particular to a D-dimer immunofluorescence quantitative kit and a preparation method thereof. Background technique [0002] D-dimer is one of the specific degradation products of cross-linked fibrin, and it is a specific metabolite of secondary fibrinolysis. Under certain pathological or physiological conditions, the dynamic balance of blood coagulation and fibrinolysis is destroyed, the blood coagulation system is activated, and the tendency of blood coagulation increases continuously, causing thrombin to convert fibrinogen into fibrin bodies, which are formed under the action of activated factor XIII Cross-linked fibrin. The fragments degraded by cross-linked fibrin under the action of plasmin are further degraded to produce fragments containing γ chains, and the γ chains are cross-linked to generate D-dimers. Therefore, the formation or increase of D-dimer reflects the activation of...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/533G01N33/577
CPCG01N33/533G01N33/577
Inventor 何平梁婷婷李冰陈润文肖丝尹
Owner ZHONGSHAN CHUANGYI BIOCHEM ENG
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