Extraction method of non-denatured protein and extraction liquid specially used by method

Abstract

The invention discloses an extraction method of non-denatured protein and extraction liquid specially used by the method. The extraction method comprises a step a2): extracting the non-denatured protein in a sample by adopting the extraction liquid, wherein the extraction liquid contains sucrose, NaCl, L-sodium ascorbate, digitonin, dithiothreitol, phenylmethylsulfonyl fluoride and a protease inhibitor. The extraction method provided by the invention is easy and convenient to operate, a reagent is mild and economic, high-quality non-denatured protein can be efficiently extracted, the method lays a foundation and provides technical support for subsequently performed adverse plant proteomics research, and the method has a certain guiding significance for extracting recalcitrant tissue protein of plants living in an extreme environment. The method has a significant application value.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for extracting non-denatured protein and a special extract thereof, in particular to a method for extracting non-denatured plant protein and a special extract thereof. Background technique [0002] As the main bearer of life activities, proteins mostly exist in the form of protein complexes and interacting proteins in cells, and their structures and functions are very complex. Traditional two-dimensional electrophoresis can only distinguish denatured proteins extracted from plants, not their state when they are functional in cells, which is not conducive to the study of protein-protein interactions and protein complexes. Protein separation is the core of electrophoresis technology, and a good protein extraction method is the premise and guarantee for obtaining intuitive protein visualization information. Up to now, the traditional method of extracting non-denatu...

AI Technical Summary

Problems solved by technology

Since the existing traditional protein extraction methods cannot meet the requirements for the extraction of non-denatured proteins from Populus euphratica leaves, especially the research on membrane proteins that require protein solubilization is even less applicable, and the introduction of non-ionic detergents will increase the pigment molecules at the same time. Risk of dissolution, severely interferes with electropherograms

Images