Cephalosporin C mushroom dreg treatment method, hydrolysate and application thereof

A technology of cephalosporin and treatment method, which is applied in the field of microbial fermentation, can solve the problems that are difficult to meet large-scale industrial application and the amount of bacteria residue is small, and achieve the effects of being suitable for large-scale industrial application, increasing production, and solving environmental pollution problems

Active Publication Date: 2018-05-18
YILI CHUANNING BIOTECH CO
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] The patent application with the publication number CN101935682A discloses a method for recycling cephalosporin C residue, which is to directly add the residue of cephalosporin C into the cephalosporin fermentation medium for use. To ferment cephalosporins, the amount of fungus residue used is small, and can only replace part of the nutrients, which is difficult to meet the requirements of large-scale industrial applications

Method used

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  • Cephalosporin C mushroom dreg treatment method, hydrolysate and application thereof
  • Cephalosporin C mushroom dreg treatment method, hydrolysate and application thereof
  • Cephalosporin C mushroom dreg treatment method, hydrolysate and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Embodiment 1 bacterial residue processing method of the present invention:

[0034] 1. Mushroom residue pretreatment method:

[0035] Collect the cephalosporin C bacteria residue, add water and stir to dilute to a water content of 40%, then hydrolyze. Adjust the pH to 7.0 to obtain the hydrolyzate of the fungus residue.

[0036] 2. Fermentation method

[0037] (1) Preparation of primary seeds

[0038] Take the slant of the Cephalosporium acremonium eggplant bottle with the preservation number ATCC36225, and prepare a 25% (mg / mL) bacterial suspension. Inoculate into the seed medium according to the inoculum amount of 1%, and cultivate at 27° C. for 50 h at a pH of 7.0.

[0039] Primary seed medium: 20g corn steep liquor, 35mL soybean oil, 13g glucose, 10g sucrose, 0.5g defoamer, 1g calcium carbonate.

[0040] (2) Preparation of secondary seeds

[0041] The primary seeds were inoculated in the medium of the secondary seeds according to 8% inoculum amount, and cultur...

Embodiment 2

[0056] Embodiment 2 Cephalosporin C fermentation method of the present invention

[0057] 1. Mushroom residue pretreatment method:

[0058] Collect cephalosporin C bacteria residue, add water and stir to dilute to water content of 35%, then hydrolyze. , to obtain the hydrolyzate of the fungus slag.

[0059] 2. Fermentation method

[0060] (1) Preparation of primary seeds

[0061] Take the slant of the Cephalosporium acremonium eggplant bottle with the preservation number ATCC36225, and prepare a 25% (mg / mL) bacterial suspension. Inoculate into the seed medium according to the inoculum amount of 1%, and cultivate at 27° C. for 50 h at a pH of 7.0.

[0062] Primary seed medium: 20g corn steep liquor, 35mL soybean oil, 13g glucose, 10g sucrose, 0.5g defoamer, 1g calcium carbonate.

[0063] (2) Preparation of secondary seeds

[0064] The primary seeds were inoculated in the medium of the secondary seeds according to 8% inoculum amount, and cultured at 27° C. for 50 hours at a ...

Embodiment 3

[0079] Embodiment 3 The method of fermentative production cephalosporin C of the present invention

[0080] 1. Mushroom residue pretreatment method:

[0081] Collect cephalosporin C bacteria residue, add water and stir to dilute to water content of 35%, then hydrolyze. , to obtain the hydrolyzate of the fungus slag.

[0082] 2. Fermentation method

[0083] (1) Preparation of primary seeds

[0084] Take the slant of the Cephalosporium acremonium eggplant bottle with the preservation number ATCC36225, and prepare a 25% (mg / mL) bacterial suspension. Inoculate into the seed medium according to the inoculum amount of 1%, and cultivate at 27° C. for 50 h at a pH of 7.0.

[0085] Primary seed medium: 20g corn steep liquor, 35mL soybean oil, 13g glucose, 10g sucrose, 0.5g defoamer, 1g calcium carbonate.

[0086] (2) Preparation of secondary seeds

[0087] The primary seeds were inoculated in the medium of the secondary seeds according to 8% inoculum amount, and cultured at 27° C...

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Abstract

The invention provides a cephalosporin C mushroom dreg treatment method. The method includes following steps: a, taking cephalosporin C mushroom dreg, and adjusting water content to 20-60%; b, adding4-8mol / L of sulfuric acid solution, maintaining at 70-90 DEG C for 10-20h, and adjusting pH to 7.0-8.0. The invention further provides hydrolysate obtained through treatment. In addition, the invention further provides a cephalosporin C culture medium and a fermentation method. By the mushroom dreg treatment method, the problem of environment pollution caused by discharging of waste mushroom dregcan be solved, good nutritional substances are provided for cephalosporin C production, production cost of cephalosporin C can be lowered greatly, and industrial application prospect is good.

Description

technical field [0001] The invention belongs to the technical field of microbial fermentation, and in particular relates to a method for treating cephalosporin C bacteria residue, a hydrolyzate and an application thereof. Background technique [0002] Antibiotic scum refers to the mycelium waste residue produced during the antibiotic fermentation production process, which belongs to industrial waste. The main components are mycelium, metabolites produced during the fermentation process, degradation products of the medium, residual organic solvents, and residual antibiotics. Fresh fungus residues are unstable, easily spoiled and give off a foul smell. In addition, once the antibiotics and their metabolite residues and resistance genes in the bacteria residues enter the environment, they may lead to the generation and spread of drug resistance of pathogenic bacteria, thus making Antibacterial drugs lose their original therapeutic effect and eventually threaten human health. H...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P35/06C12R1/75
CPCC12P35/06
Inventor 党建宁王雁庆王强杨勇
Owner YILI CHUANNING BIOTECH CO
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