Separation and screening method of trichlorphon degradation strain

Abstract

The invention provides a separation and screening method of a trichlorphon degradation strain. The method comprises the following operation steps of (1) soil collection; (2) culture medium preparation; (3) enriched domestication on the trichlorphon degradation strain by a gradient domestication method; (4) trichlorphon degradation strain separation and identification; (5) study on degradation characteristics of the strain on trichlorphon so as to analyze the biological restoration potential of the strain on the trichlorphon polluted soil. The strain screened by the method is obtained by separating in an inorganic salt solid culture medium using the trichlorphon as a unique carbon source; meanwhile, the method can be used for screening other organophosphorus pesticide degrading bacteria; the obtained strain is a soil native microorganism, belongs to a novel strain capable of degrading trichlorphon, and can be applied to the biological restoration of trichlorphon polluted soil. A gas chromatography-mass spectrometry method is used for testing the degradation effect of the strain on the trichlorphon; the operation is simple; the accuracy is high.

Description

technical field [0001] The invention relates to a screening method for trichlorfon-degrading bacterial strains, belonging to the field of microbial degradation of pesticide residues. Background technique [0002] The chemical name of trichlorfon (TCF) is O, O-dimethyl-(2,2,2-trichloro-1-hydroxyethyl), and the molecular formula is C 4 h 8 Cl 3 o 4 P, is a low-toxic organophosphorus broad-spectrum insecticide, which is widely used in agriculture to ensure the normal growth of crops. After my country banned many highly toxic organophosphorus pesticides (such as methamidophos, etc.), trichlorfon was widely used as its substitute, but the utilization rate of trichlorfon was low, generally 20-35%, and the remaining trichlorfon Residues in soil, plants and water, thus increasing the threat to the natural environment and ecosystems. [0003] Due to the long-term and large-scale use of trichlorfon, the problem of excessive pesticide residues in agricultural products such as veget...

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Problems solved by technology

[0004] For the environmental problems of trichlorfon-contaminated soil, a large number of scholars have used physical, chemical and biological treatment methods to conduct research, but physical and chemical methods are costly and prone to secondary pollution, so they are limited in the treatment of trichlorfon-contaminated soil. limit

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