Treatment method of fluorescent PCR amplification sample and kit

Abstract

The invention provides a treatment method of a fluorescent PCR amplification sample and a kit. The treatment method of the fluorescent PCR amplification sample is characterized in that an initial material and a sample treatment solution are included, wherein the sample treatment solution and the initial material are mixed to obtain a treatment product, the treatment product is directly used for the fluorescent PCR detection, the initial material is a blood sample or an oral epithelial cell sample, and the sample treatment solution is prepared from the following components: Tris-HCl, NaOH, Trixon X-100, sodium diethyl dithiocarbamate, sodium lauryl sarcosinate, NP-40, DMSO, glycine betaine and BSA. The kit comprises the sample treatment solution. The problems of the existing conventional method that the loss of DNA is great and the operation is complex due to the pre-extraction of DNA when the fluorescent PCR detection is performed can be solved. The treatment method is suitable for thefluorescent PCR detection of various uses of trace samples such as blood, oral epithelial cells and the like and is convenient in popularization.

Description

technical field [0001] The invention belongs to the field of biomedicine, and in particular relates to a processing method and a kit for fluorescent PCR amplified samples. Background technique [0002] In recent years, fluorescent PCR has been more and more widely used in various fields of clinical and inspection and quarantine due to its characteristics of accuracy, speed and convenience. Especially for the analysis of infectious diseases and genetic background, fluorescent PCR, as an accurate and reliable detection method, has become an important technical means in molecular diagnosis, clinical examination, clinical treatment and other aspects. Human genomic DNA is an important analysis sample in clinical fluorescent PCR detection. As the main biological source of genomic DNA, blood tissue or oral epithelial tissue contains a large number of PCR inhibitors, such as heme, anticoagulants, and various miscellaneous proteins, so genomic DNA needs to be extracted and purified ...

AI Technical Summary

Problems solved by technology

The purpose of these methods is to obtain relatively high-purity DNA, but at the same time, they have the disadvantages of large DNA loss, cumbersome operation, and easy cross-contamination, and often cannot meet the detection needs of some clinical high-throughput or micro-sample. There are many improvement methods. On the one hand, by changing the PCR reaction conditions or changing the DNA polymerase used in PCR, and by enhancing the tolerance of the PCR system to inhibitors to achieve fluorescent PCR amplification, although these methods do not require DNA extraction However, these methods are improvements to the PCR reaction system. In practical applications, they cannot be used in conjunction with existing commercial fluorescent PCR detection kits, so they are not conducive to popularization. On the other hand, they are The improvement of the existing methods for extracting genomic DNA achieves the purpose of rapid and high-throughput extraction of genomic DNA. These methods achieve the purpose of rapid extraction of genomic DNA through the improvement of reagents and the simplification of operation steps, but the extraction reagents used will affect the The effect of fluorescent PCR, so it still needs multiple operations such as centrifugation and transfer to complete, and the operation is relatively complicated

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