Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Phosphorescent iridium complex and preparation method and application thereof

A technology of phosphorescent iridium complexes and ligands, applied in fluorescence/phosphorescence, indium organic compounds, platinum group organic compounds, etc., can solve the problems of photobleaching and limit applications, achieve accurate detection, excellent photostability, and reduce background Effect of Signal Interference on Probe Optical Signal

Inactive Publication Date: 2018-05-29
NANJING UNIV OF POSTS & TELECOMM
View PDF0 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Currently common commercial nuclear dyes, such as dyes Hoechst and DAPI, have strong luminescence and high accuracy, but there are still some shortcomings, especially in the long-term monitoring of the cell cycle, photobleaching and self-quenching are severely limited. their application

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Phosphorescent iridium complex and preparation method and application thereof
  • Phosphorescent iridium complex and preparation method and application thereof
  • Phosphorescent iridium complex and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1 Nucleus targeting test

[0026] The digested cells were inoculated in culture dishes and cultured for 24 h. The complex was dissolved in DMSO to prepare 10 -3 Concentration of the mother solution, then dissolved in serum-free cell culture medium DMEM, diluted to imaging concentration: 5μM. Cells were imaged after incubating the complexes for 30-40 min at 37°C.

[0027] The nuclear targeting effect of the complexes is as follows figure 2 shown. Combined with the cell co-staining experiment, using 405nm laser excitation, it was observed that the complex entered the cell and was mainly concentrated in the nucleus.

Embodiment 2

[0028] Example 2 Cell cycle detection test

[0029] The digested cells were inoculated in culture dishes and cultured for 24 h. Dissolve colchicine in ultrapure water to make 10 -3 The mother solution at a concentration of g / mL was then dissolved in DMEM, a serum-free cell culture medium, and diluted to the concentration required for pretreatment (0.05-0.5 μg / mL). Under the condition of 37°C, the cells were incubated with colchicine solution for 3-6 hours, and then replaced with DMEM with serum for incubation to restore the normal cycle of the cells. The complex was dissolved in DMSO to prepare 10 -3 M concentration of the mother solution, then dissolved in serum-free cell culture medium DMEM, diluted to imaging concentration: 5μM. At the time points of each phase of the cell cycle (ie 0.5h, 5.5h, 16.5h, 21h after changing to fresh DMEM), the cells were incubated with the complex for 30 minutes at 37°C for imaging.

[0030] The effect of the complex on the cell cycle is as...

Embodiment 3

[0031] Example 3 Simultaneous detection of multiple organelles by fluorescence lifetime imaging

[0032] The digested cells were inoculated in culture dishes and cultured for 24 h. The different complexes were dissolved in DMSO respectively to form 10 -3 The stock solution of M concentration was redissolved in serum-free cell culture medium DMEM, and diluted to the imaging concentration. Cells were incubated with complexes for 30-40 min at 37°C and washed three times with PBS before imaging. Using a fluorescence lifetime imaging microscope, the location of different organelles can be distinguished through the different lifetime values ​​of each part in the imaging image.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a phosphorescent iridium complex and a preparation method and application thereof. The phosphorescent iridium complex is prepared from a metal center and ring metal ligands, and ester groups are modified in different positions. The preparation method comprises the following steps: taking 2-phenylquinoline and derivatives thereof as C^N ligands, taking bipyridyl derivativesor phenanthroline derivatives as N^N ligands, and performing coordination reaction to synthesize the phosphorescent iridium complex. The phosphorescent iridium complex can target the cell nucleus, haslight stability superior to commercial cell nucleus dye, and can perform imaging and detection on each stage in a cell cycle. The phosphorescent iridium complex can realize effective removal of short-life cell background fluorescence signals through a time resolution technology to reduce the interference of background signals to probe light signals, thereby obtaining more sensitive and reliable detection results. The phosphorescent iridium complex disclosed by the invention is a multifunctional probe integrating diagnosis and treatment, realizes accurate detection in complicated environments,and has great potential in future biomedical application.

Description

technical field [0001] The invention belongs to the technical field of biological imaging or biological detection, and relates to a complex, in particular to a class of ester group-containing ionic phosphorescent iridium complex and its application in cell imaging and / or biological detection. Background technique [0002] The cell cycle refers to the whole process that cells go through from the completion of one division to the end of the next division, usually consisting of interphase and division phase. During this process, each mother cell divides into two daughter cells on average, so the study of the cell cycle will greatly advance the understanding of eukaryotic cell division, reproduction and growth. However, during the whole cell cycle, the shape of some organelles will temporarily change or even disappear, while the nucleus is the most important organelle in eukaryotic cells and exists throughout the whole process. Good choice. [0003] At present, due to the conv...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C07F15/00C09K11/06G01N21/64
CPCC07F15/0033C09K11/06C09K2211/185G01N21/6486
Inventor 张寅徐梓涵宋林娜赵强刘淑娟黄维
Owner NANJING UNIV OF POSTS & TELECOMM
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com