Detection method of hereditary stability of gene vector for encoding muramidase-released protein and and application
A technology of genetic stability and gene carrier, applied in the field of detection of genetic stability of gene carrier, to achieve the effect of important application value
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0055] This embodiment provides a method for detecting the genetic stability of a gene carrier encoding a lysozyme-releasing protein, comprising transforming the gene carrier plasmid encoding a lysozyme-releasing protein into a host bacterial cell, and then converting the gene sequence containing the encoding lysozyme-releasing protein The host bacterium of the carrier plasmid is made into glycerol bacterium, and the genetic stability of the subcultured glycerol bacterium is identified by culturing and subculture of the glycerol bacterium.
[0056] In this example, DH5α competent cells were selected as E. coli competent cells. Of course, in other embodiments, other E. coli competent cells, such as TOP10 cells or BL21(DE3) cells, could also be selected for testing.
[0057] The specific steps of plasmid transformation are as follows:
[0058] 1.1 Take 100 μL of competent cells of Escherichia coli DH5α and add them to EP tubes, and thaw on ice;
[0059] 1.2 Take 1 μL of gene ca...
Embodiment 2
[0073] The present embodiment provides a method for detecting the genetic stability of a gene carrier encoding a lysozyme-releasing protein, and the method for detecting the genetic stability of a gene carrier encoding a lysozyme-releasing protein comprises transforming the viral plasmid of the gene carrier encoding a lysozyme-releasing protein into In the host bacteria cells, the host bacteria containing the virus plasmid of the gene carrier encoding the lysozyme releasing protein are made into glycerolbacteria, and the genetic stability of the passaged glycerolbacteria is identified by culturing and subculture of the glycerolbacteria.
[0074] In this example, TOP10 competent cells were selected as E. coli competent cells. Of course, in other embodiments, other E. coli competent cells, such as DH5α cells or BL21(DE3) cells, could also be selected for testing.
[0075] The specific steps of plasmid transformation are as follows:
[0076] 1.1 Add 100 μL of competent cells of E...
Embodiment 3
[0091] The present embodiment provides a method for detecting the genetic stability of a gene carrier encoding a lysozyme-releasing protein, and the method for detecting the genetic stability of a gene carrier encoding a lysozyme-releasing protein comprises transforming the viral plasmid of the gene carrier encoding a lysozyme-releasing protein into In the host bacteria cells, the host bacteria containing the virus plasmid of the gene carrier encoding the lysozyme releasing protein are made into glycerolbacteria, and the genetic stability of the passaged glycerolbacteria is identified by culturing and subculture of the glycerolbacteria.
[0092] In this example, TOP10 competent cells were selected as E. coli competent cells. Of course, in other embodiments, other E. coli competent cells, such as DH5α cells or BL21(DE3) cells, could also be selected for testing.
[0093] The specific steps of plasmid transformation are as follows:
[0094] 1.1 Add 100 μL of competent cells of E...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com