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Preparation method and application technology of a fluorescent probe for detecting glutathione

A fluorescent probe and glutathione technology, which is applied in the field of fluorescent probe sensing in analytical chemistry, can solve the problems of cumbersome operation, low sensitivity, and expensive instruments, and achieve the effect of simple synthesis, high sensitivity, and real-time detection

Active Publication Date: 2020-05-19
HUBEI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the detection of GSH concentration in complex actual samples is often restricted by various factors and cannot obtain good detection results. The traditional GSH concentration detection methods mainly include spectrophotometry, enzyme cycling, colorimetry, and liquid phase. Chromatography, etc.
Although these methods can realize the qualitative or quantitative detection of GSH, there are certain defects, such as complicated sample preparation, expensive instruments, cumbersome operation, long detection cycle, poor selectivity, low sensitivity, and not easy to popularize and apply, etc.

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  • Preparation method and application technology of a fluorescent probe for detecting glutathione
  • Preparation method and application technology of a fluorescent probe for detecting glutathione
  • Preparation method and application technology of a fluorescent probe for detecting glutathione

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Embodiment 1

[0023] In an anhydrous, nitrogen-protected environment, 1,2-diaminoanthraquinone (0.6g, 2.5mmol) was dissolved in tetrahydrofuran (15mL), and then slowly added dropwise (0.7mL, 9.7mmol) thionyl chloride and 1.5mL of triethylamine was stirred overnight at room temperature, and a purple solid was formed in the reaction. After the reaction was completed, the reaction solution was poured into 100 g of crushed ice, deep cooled, filtered to obtain the crude product, recrystallized from glacial acetic acid, and recrystallized from ethanol to obtain the product ATD (0.73 g, 2.3 mmol). Dissolve ATD (0.5g, 1.6mmol) in 18mL of glacial acetic acid, heat and stir to 90°C, then add 8mL of concentrated hydrochloric acid. Then add KClO several times in small amounts 3 (3.0g, 22.4 mmol), and then continue to react at 90°C for 4 hours. After the reaction is completed, stop heating, and after cooling to room temperature, pour the reaction solution into ice water, filter to obtain the crude prod...

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Abstract

The invention belongs to the technical field of fluorescent probe sensing in analytical chemistry and relates to a preparation method of a novel fluorescent probe based on an anthraquinone structure and an application technology of the novel fluorescent probe to detection of glutathione (GSH). The preparation method comprises the step of obtaining a probe molecule from a thiadiazole anthraquinonederivative and potassium chlorate through chlorination reaction; the preparation method is simple and feasible, the cost is low and the prepared probe has a stable structure. Under moderate conditions, the probe can be applied to high-sensitivity, high-selectivity, and rapid and visualized detection of the glutathione. Moreover, the probe is successfully used for detecting the glutathione in biological cells. The preparation method and the application technology of the novel fluorescent probe for detecting the glutathione, provided by the invention, have a very good application prospect.

Description

technical field [0001] The invention relates to the technical field of fluorescent probe sensing in analytical chemistry, and relates to a novel high-selectivity, high-sensitivity, fast and visual fluorescent probe preparation method for glutathione detection and its application technology. Background technique [0002] Glutathione (GSH) is a small molecular peptide composed of three amino acids, cysteine, glutamic acid and glycine, which exists in almost every cell of the body. GSH plays a very important role in living organisms. It can help living organisms maintain normal immune system functions, and has anti-oxidation and integrated detoxification effects. Physiological level of GSH content helps to remove free radicals in the body, not only enhance immunity, anti-oxidation, anti-aging effects, but also play a role in the protection of the liver, the protection of damaged organs, anti-radiation and the treatment of some diseases It plays an irreplaceable role, and abnor...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07D285/14C09K11/06G01N21/64
CPCC07D285/14C09K11/06C09K2211/1051G01N21/6486
Inventor 刘恒陈逢灶张修华王升富
Owner HUBEI UNIV