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A highly efficient phorbol ester degrading strain and its application in detoxification of Jatropha curcas cake fermentation

A technology of jatropha curcas cake and phorbol ester, which is applied in the direction of bacteria, microorganism-based methods, microorganisms, etc., can solve the problems of nutrient composition destruction, low efficiency of physical detoxification, and chemical residues, and achieve balance of amino acid content, The effect of increasing feed value

Inactive Publication Date: 2020-03-10
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The efficiency of physical detoxification is low, and it will cause certain damage to the nutritional components in the cake; the chemical detoxification operation is complicated and cumbersome, and it is easy to cause chemical residues, and the detoxification range is small and the cost is high

Method used

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  • A highly efficient phorbol ester degrading strain and its application in detoxification of Jatropha curcas cake fermentation
  • A highly efficient phorbol ester degrading strain and its application in detoxification of Jatropha curcas cake fermentation
  • A highly efficient phorbol ester degrading strain and its application in detoxification of Jatropha curcas cake fermentation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Soil samples were obtained from 5-10 cm below the soil surface of Jatropha curcas root system in the Jatropha curcas plantation area of ​​Panzhihua City, Sichuan Province. After enrichment, screening, purification and cultivation, 28 strains that could tolerate phorbol ester in Jatropha curcas cake were obtained. strain. The obtained strains were inoculated into Jatropha curcas cakes for shaking flask culture, and the strains with the best degradation effect on phorbol esters were screened out by measuring the content of phorbol esters in the fermented cakes, which were recorded as Enterobacter Z11, The colony growth results of the strain on LB medium are as follows: figure 1 shown. The scanning electron microscope image of Enterobacter Z11 is shown in figure 2 .

[0028] Gram staining and physiological and biochemical identification were performed on the strain numbered Z11, and Z11 was determined to be Enterobacter cloacae according to the color reaction. Some r...

Embodiment 2

[0037] The three factors of inoculation amount, initial water addition and fermentation time were selected for the experiment, and the experimental factors and levels are shown in Table 2.

[0038] Table 2 Factors and levels of experimental design

[0039]

[0040]

[0041] The research object of the experiment is the degradation rate of phorbol ester, which is recorded as variable Y, and the three factors of inoculum size, initial water content and fermentation time are the research objects, which are respectively recorded as variables X1, X2, and X3. Design 3 factors and 3 levels (inoculum size 15%, 20%, 30%; initial water content 30%, 50%, 80%; fermentation time 4d, 5d, 6d) fermentation experiment. The results of the 17 groups of tests are shown in Table 3.

[0042] Table 3 Response surface design and measured value of phorbol ester degradation rate

[0043]

[0044]

Embodiment 3

[0046] Inoculate the activated Enterobacter Z11 in the Jatropha curcas solid-state fermentation medium (after drying the Jatropha curcas cake, pass through 40 mesh, take 5g, add 5mL of deionized water, natural pH, sterilize at 121°C for 20min), and inoculate the strain Under the conditions of 20% dosage and 50% humidity, the fermentation was carried out for 5 days. After the fermentation, the content of anti-nutritional factors in the Jatropha curcas cake before and after fermentation was detected. The results are shown in Table 4. According to the results, the phorbol ester content in the Jatropha curcas cake decreased by 51.6%, the phytic acid content decreased by 82.56%, the tannin content decreased by 37.80%, the trypsin inhibitor content decreased by 90.45%, and the lectin content decreased by 88.90%.

[0047] Table 4 Contents of anti-nutritional factors of Jatropha curcas cake before and after fermentation

[0048]

[0049] According to the above experimental results,...

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Abstract

The invention relates to a functional strain, and relates to a high-efficiency degrading phorbol ester strain and its application in detoxification of Jatropha curcas cake fermentation. A highly efficient phorbol ester degrading strain, the bacteria is Enterobacter cloacae ( Enterobacter cloacae ) strain Z11, which was collected on September 21, 2017 in the General Microbiology Center of the China Committee for the Collection of Microbial Cultures (Address: No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing, Institute of Microbiology, Chinese Academy of Sciences, Zip Code 100101) Deposited, the number is CGMCC No.14655. The invention can effectively reduce the content of toxic components and anti-nutritional factors in the Jatropha curcas cake, and can improve the feeding value of the Jatropha curcas cake.

Description

technical field [0001] The invention relates to a functional strain, and relates to a high-efficiency degrading phorbol ester strain and its application in detoxification of Jatropha curcas cake fermentation. Background technique [0002] Jatropha curcas (Jatropha curcas) belongs to Euphorbiaceae (Euphorbiacea) Jatropha (Jatropha) plants, divided into poisonous and non-toxic two gene types, of which the non-toxic species are only distributed in Mexico. The seed of Jatropha curcas is the fruit of Jatropha curcas, and the oil content of its seed kernel is as high as 60%, which is an ideal raw material for preparing biodiesel. However, because the cake contains anti-nutritional factors and toxins such as trypsin inhibitors, lectins, and phorbol esters, it cannot be directly used as animal feed. At present, it is generally discarded as waste, which not only causes a lot of waste of resources, but also pollutes the environment. In recent years, due to worldwide research on the ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A23L5/20C12R1/01
CPCA23L5/28C12N1/20C12N1/205C12R2001/01
Inventor 刘建新赵一诺汪海峰
Owner ZHEJIANG UNIV
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