Raman super-resolution microimaging system based on structured light illumination and imaging method
A technology of structured light illumination and microscopic imaging, which is applied in Raman scattering, material analysis by optical means, and material analysis, etc., can solve the problems of inability to quickly image the whole surface of the sample, photodamage of the sample by fluorescence bleaching, and poor stability, etc. Achieve high-speed super-resolution Raman imaging, high sensitivity, and low requirements
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[0035] Attached below Figure 1-6 The present invention is described in further detail.
[0036] Such as Figure 5 As shown, the Raman super-resolution microscopy imaging system based on structured light illumination includes a structured light generation device, a Raman signal excitation device and a disposable Raman imaging device.
[0037] Among them, such as figure 1 As shown, the structured light generating device includes a laser 1, an excitation filter 2, a beam expander group 3, a PBS beam splitter 4, a half-wave plate 5, a ferroelectric liquid crystal 6, a polarization modulation component 7, and a first focusing lens 8 and mask board9. The laser 1 emits monochromatic excitation light, which is filtered by the excitation filter 2 in turn. After the beam expander group 3 expands the beam, it enters the PBS beam splitter 4 for beam splitting. The polarized light in the S direction passes through the half-wave plate 5 and reaches the ferroelectric liquid crystal. 6. ...
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