Penicillium purslane and its application in the preparation of anti-Ralstonia solanacearum medicine
A technology against Penicillium sclerotiorum and R. solanacearum, which is applied in the fields of application, microbial-based methods, chemicals for biological control, etc., and can solve problems that have not been reported.
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Embodiment 1
[0022] Penicillium sclerotiorum jyscaumcx01 of the present invention is isolated from the stems, leaves and roots of purslane, and the specific separation method is: washing the stems and roots of purslane with tap water → drying → Soak in 75% alcohol for 2 minutes → rinse with sterile water → soak in 0.1% mercuric chloride for 1.5 minutes → rinse with sterile water, and the sterile water-coated plate (PDA) cleaned for the last time was used as a blank control.
[0023] Wash leaves with tap water → dry → soak in 75% alcohol for 2 minutes → rinse with sterile water → soak in 0.1% mercuric chloride for 30 seconds → rinse with sterile water, and use the sterile water-coated plate (PDA) for the last cleaning as a blank control .
[0024] The endophytic fungus was firstly obtained from the root, and after several times of scratching, the pure endophytic fungus was finally obtained, which was named as jyscaumcx01.
[0025] Colony morphology of bacteria jyscaumcx01 in different medi...
Embodiment 2
[0027] Inoculate the endophytic fungus Penicillium sclerotiorum (Penicillium sclerotiorum) jyscaumcx01 into the PDA medium, culture at 28°C for 7 days at a shaker speed of 110 r / min, and obtain the sclerotium of the endophytic fungus of purslane Penicillium culture. The Penicillium sclerotinum culture of the purslane endophytic fungus is centrifuged, and the supernatant is taken as the fermentation liquid.
[0028] Get different volumes of fermentation broth and add it to 10mL SMSA medium with a R. solanacearum concentration of 0.05 (OD value at 600nm), and use PDA medium to make up 12ml of culture fluid volume, 110r / min, 28 ° C for 2 days and measure the 600nm OD value, repeated 3 times, the specific results are as follows image 3 Shown (the unit of the abscissa is microliter). From image 3 It can be seen that with the increase of the amount of fermentation broth, the effect of inhibiting R. solanacearum becomes more and more obvious.
Embodiment 3
[0030] Inoculate the endophytic fungus Penicillium sclerotiorum (Penicillium sclerotiorum) jyscaumcx01 into the PDA medium, culture at 28°C for 7 days at a shaker speed of 110 r / min, and obtain the sclerotium of the endophytic fungus of purslane Penicillium culture. The Penicillium sclerotinum culture of the purslane endophytic fungus is centrifuged, and the supernatant is taken as the fermentation liquid. The fermentation broth was extracted with an equal volume of ethyl acetate, the ethyl acetate extract was concentrated and evaporated under reduced pressure, and the ethyl acetate was removed to obtain an ethyl acetate extract. The ethyl acetate extract was prepared into 0.1mg / ml, 0.5mg / ml, 1mg / ml, 5mg / ml, 10mg / ml solutions with chromatographic methanol, the control 1 used 1mg / ml streptomycin methanol solution, and the control 2 was Methanol alone. Add 10 μl of each experimental sample into a petri dish with R. solanacearum, do 3 repetitions, and record the diameter of the...
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