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Method for preparing oligopeptide powder from antarctic krill shells

A technology of Antarctic krill and shrimp shell powder, applied in the direction of fermentation, can solve the problems of complex pretreatment steps, and achieve the effects of uniform distribution, small molecular weight and high yield

Inactive Publication Date: 2018-06-08
ZHEJIANG OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

For example, the Chinese patent with the application number 201310338921.4 discloses a preparation process of fish skin collagen oligopeptide, which can obtain collagen oligopeptide powder with a purity greater than 90% and a molecular weight of less than 1000 Da, but the pretreatment steps are complicated and require separate soaking in acid and alkali solutions, and Post-production steps that require membrane purification
The Chinese patent application number 201510903014.9 discloses a preparation process of yak bone collagen oligopeptides, which can obtain yak bone collagen oligopeptides with a purity greater than 90% and a molecular weight of less than 1000Da, without the need for membrane purification steps, but this process still requires an acid-base solution pre-processing

Method used

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  • Method for preparing oligopeptide powder from antarctic krill shells
  • Method for preparing oligopeptide powder from antarctic krill shells

Examples

Experimental program
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Effect test

Embodiment 1

[0024] Antarctic krill shells were washed with water, dried, and pulverized by a grinder to obtain shrimp shell powder; after mixing the shrimp shell powder and water at a mass ratio of 1:5, adjusting the pH to 7.0, keeping it warm at 50°C for 10 minutes, and adding it into shrimp shell Compound protease with 0.3% powder mass, keep warm for 6 hours, heat up to 95°C to inactivate enzyme for 15 minutes, cool to 50°C; adjust pH to 3.5, add acid protease with 0.1% shrimp shell powder mass, keep warm at 35°C for 6 hours , the temperature was raised to 85°C to inactivate the enzyme for 20 minutes to obtain the enzymolysis solution; the pH of the enzymolysis solution was adjusted to neutral, and the supernatant was collected after standing at 20°C for 8 hours; the outlet air temperature was 30°C, and the inlet air temperature was 180°C for spray drying Afterwards, oligopeptide powder was obtained.

[0025] Measure the protein recovery rate, peptide content, peptide content of molecul...

Embodiment 2

[0031] Antarctic krill shells were washed with water, dried, and pulverized by a grinder to obtain shrimp shell powder; after mixing the shrimp shell powder and water at a mass ratio of 1:10, adjusting the pH to 8.0, keeping it at 40°C for 20 minutes, and adding it into shrimp shells Mix protease with 0.1% mass of powder, keep warm for 8 hours, heat up to 85°C to inactivate enzyme for 20 minutes, cool to 60°C; adjust pH to 2.5, add acid protease with 0.3% mass of shrimp shell powder, keep warm at 40°C for 4 hours , the temperature was raised to 95°C to inactivate the enzyme for 10 minutes to obtain the enzymolysis solution; the pH of the enzymolysis solution was adjusted to neutral, and the supernatant was collected after standing at 5°C for 20 hours. The outlet air temperature was 40°C, and the inlet air temperature was 160°C. Afterwards, oligopeptide powder was obtained.

[0032] Measure the protein recovery rate, peptide content, peptide content of molecular weight 50 ) and...

Embodiment 3

[0034] Antarctic krill shells were washed with water, dried, and pulverized by a grinder to obtain shrimp shell powder; after mixing the shrimp shell powder and water at a mass ratio of 1:7, adjusting the pH to 7.5, keeping it at 45°C for 15 minutes, and adding it into shrimp shell Compound protease with 0.2% mass of powder, keep warm for 7 hours, heat up to 90°C to inactivate enzyme for 18 minutes, cool to 55°C; adjust pH to 3.0, add acid protease with 0.2% mass of shrimp shell powder, heat at 37°C for 5 hours , heat up to 90°C to inactivate the enzyme for 15 minutes to obtain the enzymolysis solution; adjust the pH of the enzymolysis solution to neutral, and collect the supernatant after standing at 10°C for 15 hours. The outlet air temperature is 35°C, and the inlet air temperature is 170°C. Afterwards, oligopeptide powder was obtained.

[0035] Measure the protein recovery rate, peptide content, peptide content of molecular weight 50 ) and antibacterial effect, the results...

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Abstract

The invention relates to a preparation method of oligopeptide powder, and discloses a method for preparing the oligopeptide powder from antarctic krill shells. The method comprises the following stepsof (1) crushing; (2) primary enzymolysis: using composite prolease; (3) secondary enzymolysis: using acid prolease; (4) drying. The preparation method has the advantages that the process is very simple; the pretreatment by acid and alkali solutions is not needed; the film filtering posttreatment is not needed; a required product can be obtained only by performing twice hydrolysis by composite prolease and acid prolease; meanwhile, the consumption of the prolease is low; the production cost is greatly reduced; the obtained oligopeptide has high yield and small molecular weight. The yield of the oligopeptide powder is 240 to 300mg / g raw materials; the peptides with the molecular weight being smaller than 1000Da is 85 to 90 weight percent, so that the preparation method provided by the invention is very applicable to industrial production.

Description

technical field [0001] The invention relates to a method for preparing oligopeptide powder, in particular to a method for preparing oligopeptide powder by using Antarctic krill shells. Background technique [0002] Although our country is very rich in shrimp resources, the heads and shells, which account for nearly 40% of the shrimp weight, become solid wastes during the processing and eating process of shrimps, most of which are discarded or used to produce feed, causing serious environmental damage. Pollution reduces the utilization value of shrimp heads and shells. The molecular weight of oligopeptides is generally below 1000 Daltons, which can be directly absorbed by the human body without digestion. The crude protein content in Antarctic krill shrimp shells exceeds 50%, which is a good material for preparing oligopeptide powder. [0003] The existing use of Antarctic krill is only to prepare polypeptides. The Chinese patent application number 201710201402.1 discloses a...

Claims

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Application Information

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IPC IPC(8): C12P21/06
CPCC12P21/06
Inventor 刘宇姜维胡世伟李世杰
Owner ZHEJIANG OCEAN UNIV
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