Preparation method of heavy metal zinc artificial antigen and application of NOTA in preparation of heavy metal zinc artificial antigen reagent

An artificial antigen and heavy metal technology, applied in the field of heavy metal ion immunochemistry, can solve the problems of weak functional group characteristics, weak antigenic determinant antigen characteristics, affecting the preparation efficiency of high specificity and high sensitivity antibodies, etc. The effect of strong specificity and high antiserum titer
CN108148129AActive Publication Date: 2018-06-12ZHEJIANG GONGSHANG UNIVERSITY
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
ZHEJIANG GONGSHANG UNIVERSITY
Publication Date
2018-06-12

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Abstract

The invention discloses a preparation method of heavy metal zinc artificial antigen, which applies 2-S-(4- aminobenzene)-1, 4, 7, 10 tetraazacyclo cyclononane-1, 4, 7, 10-acetate tetrahydrate(p-NH2-Bn-NOTA, NOTA for short) as cheating agent, and couples mercury ion chelating agent composite with carrier protein bovine serum albumin BSA or egg albumin OVA; thus the artificial antigen is prepared. The method adds the sodium borohydride processing step on the basis of traditional method in the past, and improves the coupling efficiency and antiserum titre. The invention further discloses an application of NOTA in preparation of the heavy metal zinc artificial antigen.
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Description

Technical field

[0001] The invention belongs to the technical field of heavy metal ion immunochemistry, and specifically relates to a preparation method of heavy metal zinc artificial antigen and the application of NOTA in the preparation of heavy metal zinc artificial antigen reagent. Background technique

[0002] Heavy metal pollution mainly refers to the pollution of lead, cadmium, mercury, nickel, chromium, arsenic, zinc, copper and other pollutants to the environment. Heavy metals are widely distributed and difficult to degrade. They can enter the human body through the atmosphere, water, and food chain, and interact with proteins and various enzymes in the body to make them inactive and accumulate in certain organs. If they exceed the limit that the human body can tolerate , Can cause acute or chronic poisoning of human body, have carcinogenic, teratogenic and mutagenic effects, and have great harm to human body. Therefore, strengthening the detection of heavy metal residu...

Claims

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