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Integrated drug screening and staining method based on micro-fluidic chip

The technology of a microfluidic chip and a dyeing method is applied in the field of integrated drug screening and dyeing based on the microfluidic chip, and can solve the problems of a large amount of consumption and complicated and complicated operation steps.

Active Publication Date: 2018-06-12
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In order to solve the technical limitations of the previous cell drug screening and staining process, such as cumbersome and complicated operation steps and the consumption of a large amount of reagents

Method used

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  • Integrated drug screening and staining method based on micro-fluidic chip
  • Integrated drug screening and staining method based on micro-fluidic chip
  • Integrated drug screening and staining method based on micro-fluidic chip

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0086] An integrated drug screening and dyeing method based on a microfluidic chip. The integrated drug screening and dyeing microfluidic chip is composed of an upper layer, a lower layer, and a bottom layer, which are arranged in series in series, wherein: the upper layer is a liquid circuit control layer, the lower layer is the gas circuit control layer, and the bottom is a blank glass bottom plate;

[0087] The liquid path control layer is specifically provided with the following structure:

[0088] ——Cell fluorescence dyeing inlet: located at the most upstream of the entire fluid circuit control layer;

[0089] ——Fluorescence dyeing injection channel area P1: arranged between the cell fluorescence dyeing injection port and the cell injection channel area P2 for communication between the two;

[0090] ——Cell sampling port: it is provided with at least two, and each cell sampling port is set on one of the channels in the cell sampling channel area P2, and each channel in th...

Embodiment 2

[0145] An integrated drug screening and dyeing method based on a microfluidic chip. The integrated drug screening and dyeing microfluidic chip is composed of an upper layer, a lower layer, and a bottom layer, which are arranged in series in series, wherein: the upper layer is a liquid circuit control layer, the lower layer is the gas circuit control layer, and the bottom is a blank glass bottom plate;

[0146] The liquid path control layer is specifically provided with the following structure:

[0147] ——Cell fluorescence dyeing inlet: located at the most upstream of the entire fluid circuit control layer;

[0148] ——Fluorescence dyeing injection channel area P1: arranged between the cell fluorescence dyeing injection port and the cell injection channel area P2 for communication between the two;

[0149] ——Cell sampling port: it is provided with at least two, and each cell sampling port is set on one of the channels in the cell sampling channel area P2, and each channel in th...

Embodiment 3

[0190] The SU-8 template with channel part protrusions was prepared by photolithography and etching. The upper and lower structures of the chip were respectively composed of two SU-8 template anti-mold PDMS; Make PDMS easy to peel off the bottom surface of the template; PDMS and initiator are mixed evenly at a volume ratio of 10:1, poured on the upper and lower structure SU-8 templates of the chip respectively, and cured in an oven at 80°C for 40 minutes, and the PDMS and the upper SU-8 template of the chip are peeled off. Obtain a PDMS chip with a structure; treat the upper layer of the chip with the structure with the lower layer of the chip without the structure on the SU-8 template for 2 minutes, heat bake at 80°C for 1 hour, and seal irreversibly; seal the above The PDMS chip was peeled off from the SU-8 template of the underlying structure, treated with oxygen plasma for 2 minutes with a clean blank glass slide, and then baked at 80°C for 1 hour for irreversible sealing, ...

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Abstract

The invention discloses an integrated drug screening and staining method based on a micro-fluidic chip. The micro-fluidic chip is such structured that a liquid path control layer is arranged on the upper layer of the micro-fluidic chip, a gas path control layer is arranged on the lower layer, and a blank glass base plate is arranged on the bottom surface of the micro-fluidic chip. The integrated drug screening and staining method based on the micro-fluidic chip sequentially comprises the following steps: (1) implementing chip pre-processing; (2) inoculating and cultivating cells; (3) implementing drug stimulation; and (4) implementing fluorescent staining. Inlets of all liquid path layers are independently controlled via a valve of a gas path layer; and cultivation of different types of cells, various drug stimulation and different antibody staining can be simultaneously implemented. According to the integrated drug screening and staining method provided by the invention, drug screening and fluorescent staining on the micro-fluidic chip can be achieved via micro-fluid and micro-valve technologies in the micro-fluidic chip, so that a completely new technology platform is provided for researches on cell culture, cell in-situ fluorescent staining and drug staining. The method provided by the invention is simple and convenient to operate, low in cell and reagent dosages, high in integration and broad in application scope.

Description

technical field [0001] The invention relates to the cross-application technical field of microfluidic technology and cell biology, and in particular provides an integrated drug screening and staining method based on a microfluidic chip. Background technique [0002] In the prior art, a microfluidic chip is a system that manipulates the flow of a small volume of fluid in a tiny channel, where the scale of the channel is tens to hundreds of microns, and the amount of carrying fluid is 10 -9 ~10 -18 L. The various operating units of the microfluidic chip are connected to each other through the flow of fluid in the microchannel network. Microfluidic control is the core of microfluidic lab-on-a-chip operations, and the processes involved in sampling, mixing, reaction, and separation are all completed in the movement of controllable fluid. Whether it is macroscopic or microfluidic, valves are the core components of fluidic control. Due to its importance, the development of mic...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M3/00C12M1/34B01L3/00
CPCG01N33/5008C12M23/16B01L3/5027G01N2500/10C12N2503/02
Inventor 秦建华张晓庆姜雷苏文涛石杨
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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