High-solubility soybean peptide as well as preparation technology and application thereof

A soybean polypeptide, high solubility technology, used in applications, protein food processing, vegetable protein processing and other directions, can solve the problems of limited solubility, reduced protein and polypeptide content in products, and easy to produce precipitation.

Active Publication Date: 2018-06-15
武汉天天好生物制品有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The preparation of soybean peptides by enzymatic hydrolysis in the prior art mainly uses one or more enzymes of alkaline protease, neutral protease and trypsin for enzymolysis and preparation. According to the requirements of GB22492-2008 "Soybean Peptide Powder", soybean The protein content of peptide products is ≥80%, and the peptide content is ≥55%. However, the solubility of soybean peptide products currently on the market is limited under high temperature an

Method used

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  • High-solubility soybean peptide as well as preparation technology and application thereof

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Experimental program
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Effect test

Embodiment 1

[0044] This embodiment provides a highly soluble soybean polypeptide, which is prepared by the following preparation process:

[0045] Weigh 1 kg of soybean protein isolate and 15 kg of distilled water, mix and stir evenly, and the rotating speed of mixing and stirring is 60 r / min to prepare soybean protein isolate slurry.

[0046] Raise the temperature of soybean protein isolate slurry to 50°C, add 0.015kg of alkaline protease under natural pH conditions for the first enzymatic hydrolysis for 2 hours; then add 0.015kg of neutral protease for the second enzymatic hydrolysis for 3 hours; then add citric acid Adjust the pH to 5.4-6.2, add 0.003 kg of acidic protease, and perform the third enzymatic hydrolysis for 1 hour to obtain an enzymatic hydrolysis solution.

[0047] Heat the enzymolysis solution to 95°C and keep it for 18 minutes to inactivate the enzyme activity.

[0048] Cool the enzymolysis solution after inactivation to 65°C, add 4% of its mass of activated carbon, st...

Embodiment 2

[0050] This embodiment provides a highly soluble soybean polypeptide, which is prepared by the following preparation process:

[0051] Weigh 1kg soybean protein isolate and 12kg distilled water, mix and stir evenly, the rotating speed of mixing and stirring is 70r / min, make soybean protein isolate slurry.

[0052] Raise the temperature of soybean protein isolate slurry to 55°C, add 0.03kg of alkaline protease under natural pH conditions for the first enzymolysis for 0.3h; then add 0.03kg of neutral protease for the second enzymolysis for 2h; then add apples Adjust the pH to 5.4-6.2 with acid, add 0.005 kg of acidic protease, and carry out the third enzymolysis for 0.5-2 hours to obtain an enzymolysis solution.

[0053] Heat the enzymatic solution to 100°C and keep it for 15 minutes to inactivate the enzyme.

[0054] Cool the enzymolysis solution after inactivation to 70°C, filter the enzymolysis solution through a roll-type ultrafiltration membrane purification system with a ...

Embodiment 3

[0056] This embodiment provides a highly soluble soybean polypeptide, which is prepared by the following preparation process:

[0057] Weigh 1kg soybean protein isolate and 18kg distilled water, mix and stir evenly, the rotating speed of mixing and stirring is 40r / min, make soybean protein isolate slurry.

[0058] Raise the temperature of soybean protein isolate slurry to 50°C, add 0.008kg alkaline protease under natural pH conditions, and carry out the first enzymatic hydrolysis for 1 hour; then add 0.008kg neutral protease, and carry out the second enzymatic hydrolysis for 5 hours; then add acetic acid to adjust The pH is 5.4-6.2, add 0.001kg of acidic protease, carry out the third enzymolysis for 2 hours, and obtain the enzymolysis solution.

[0059] Heat the enzymolysis solution to 90°C and keep it for 20 minutes to inactivate the enzyme activity;

[0060] Cool the enzymatic hydrolysis solution after inactivation to 60°C, add 6% of its mass activated carbon, stir for 25 m...

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Abstract

The invention provides high-solubility soybean peptide as well as a preparation technology and application thereof, and relates to the field of a processing technology of soybean protein. The preparation technology of the high-solubility soybean peptide comprises the following steps of uniformly mixing and stirring soy protein isolate with distilled water; warming soy protein isolate slurry to 50-55 DEG C, adding alkali protease under natural pH condition for enzymolysis, then adding neutral protease for enzymolysis, then adding organic acid for regulating the pH to 5.4-6.2, and adding acid protease for enzymolysis so as to obtain enzymatic hydrolysate; performing enzyme deactivation on the enzymatic hydrolysate; and lowering the temperature to 60-70 DEG C, performing physical bitterness removing treatment, performing concentration, and performing spray drying. According to the preparation technology, the organic acid is used for regulating the pH during acidic enzymolysis, so that theenzymolysis effect is guaranteed, besides, the labor cost is saved, and the preparation technology is simple and convenient to operate, and is suitable for scaled production. The prepared high-solubility soybean peptide is high in solubility under the acidic and high-temperature high-pressure condition, and is free from bitterness. The high-solubility soybean peptide can be made into health-carefoods, functional foods and foods.

Description

technical field [0001] The invention relates to the field of soybean protein processing technology, and in particular to a highly soluble soybean polypeptide and its preparation process and application. Background technique [0002] At present, for the deep processing of soybean protein isolate, methods such as acid method, alkali method or enzymatic hydrolysis are generally used to transform the macromolecular protein in soybean decomposed protein into small molecular polypeptides that can be directly absorbed by the human body. The method of enzymatic hydrolysis The most widely used. At present, many patented technologies on the enzymatic hydrolysis process of soybean polypeptide have been disclosed, most of which focus on the enzymatic hydrolysis process of alkaline protease and the debittering process. For example, the Chinese invention patent application with publication number CN1359693A discloses a production process of soybean polypeptide dry powder with lipid-lower...

Claims

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Application Information

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IPC IPC(8): A23J3/16A23J3/34
CPCA23J3/16A23J3/346
Inventor 陈大伟成静陈栋梁
Owner 武汉天天好生物制品有限公司
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